Effects of diphencyprone on expression of Bcl-2 protein in patients with Alopecia areata

Ajami, Marjan; Babakoohi, Shahab; Khaki, Pejvak; Habibey, Rouhollah; Akhiani, Maryam; Hassan, Sk. Sarif; Firooz, Alireza

doi:10.3109/08923970903440200

Cited by 12 publications

(4 citation statements)

References 12 publications

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“…Previous results have shown that mTOR phosphorylation can decrease ischemia-induced cellular damage. In accordance with the previous evidence, rosmarinus can activate mTOR/S6K and PI3kinase-akt-mTOR pathways to improve wound healing (9).…”

Section: Introductionsupporting

confidence: 91%

Rosmarinus Improved Skin Flap Survival Through mTOR Dependent Pathway

Javid

Norouzy

Bangaleh

et al. 2018

Serbian Journal of Dermatology and Venereology

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Introduction: Skin flap application in the clinical practice is restricted due to the ischemic damage and flap necrosis. Rosmarinus oil has been shown to improve a skin flap survival. In the present work we studied the role of mammalian target of rapamycin (mTOR) signaling on rosmarinus-induced flap protection. Methods: A flap surgery was performed on Sprauge-Dawley rats (8 cm in by 3 cm). A week before and a week after the surgery the flaps were treated with topical rosmarinus oil (twice per day). Rapamycin (m-TOR inhibitor) was administered 30 minutes before the flap surgery in rosmarinus-treated or not treated groups. A week after the surgery the malondialdehyde (MDA) contents, myeloperoxidase (MPO) and superoxide dismutase (SOD) activities, expression of Bax, Bcl-2, mTOR and p-mTOR were measured in the flap tissue. Results: Topical application of the rosmarinus increased the flap survival (p<0.05), anti-oxidative enzyme activity (SOD, p<0.05) and anti-apoptotic protein Bcl-2 expression. Rosmarinus treatment decreased the flap MDA content, MPO activity, and pro-apoptotic protein Bax expression (p<0.05). Rosmarinus topical application did not change mTOR expression and phosphorylation in the flap tissue. Expression of p-mTOR in rosmarinus treated group was suppressed by rapamycin pre-treatment, which also abolished rosmarinus effects on the flap survival (p<0.05). Conclusion: These data suggested p-mTOR dependent mechanism in rosmarinus-induced flap survival.

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Section: Introductionsupporting

confidence: 91%

Rosmarinus Improved Skin Flap Survival Through mTOR Dependent Pathway

Javid

Norouzy

Bangaleh

et al. 2018

Serbian Journal of Dermatology and Venereology

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“…Deregulation of apoptosis at catagen could induce the abnormality of hair follicle cycle and hair growth disorders (Stenn et al 1996;Paus 1996). Identifying the factors that regulate catagen apoptosis is beneWcial not only to reveal the mechanism of catagen formation (Fan et al 2010;Lin et al 2011), but also to Wnd a new target for hair growth disorder treatment (Pazoki-Toroudi et al 2010). But until now, there has been very little information about the factors that control the catagen phase.…”

mentioning

confidence: 99%

Gsdma3 gene is needed for the induction of apoptosis-driven catagen during mouse hair follicle cycle

Lei

Gao

Yang

et al. 2011

Histochem Cell Biol

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Gsdm is a newly found gene family, which is restricted in its expression to the gastrointestinal tract and the skin epithelium. As a main member of the Gsdma subfamily, Gsdma3 is expressed specifically in the hair follicle of mouse skin, but its function remains largely unclear. By hematoxylin and eosin staining, we showed that Gsdma3 gene mutation caused an abnormal catagen phase with unshortened length and unshrunk structure of the hair follicle, in which the development of catagen phase was inhibited. TUNEL staining further revealed that the apoptosis of the hair follicle was obviously decreased in mutant mice. Caspase-3 downregulation was also detected by immunofluorescence, Western blot and RT-PCR in the hair follicle of the mutant mice. After intradermal injection of Gsdma3 gene expression plasmid, apoptosis as well as Caspase-3 expression in the hair follicle of mutant mice was enhanced, and so the catagen retardation of Gsdma3-mutant mice was rescued. Our results confirmed that Gsdma3 gene mutation interfered with catagen formation during mouse hair follicle cycle and, by upregulation of Caspase-3 expression and promotion of apoptosis, Gsdma3 gene could play an essential role in normal catagen induction.

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“…During reperfusion, nitric oxide (NO) and ROS production enhance germ cell‐specific necrosis. On the other hand, excessive generation of the ROS is tightly associated with the increased vulnerability of the cells to apoptosis (Kaur & Bansal, ; Mukherjee, Haldar, & Vishwas, ; Pazoki‐Toroudi, Ajami, et al., ; Pazoki‐Toroudi, Ajami, Babakoohi, et al., ). In present work, we evaluated the effect of capsaicin on pro‐apoptotic and anti‐apoptotic factors.…”

Section: Discussionmentioning

confidence: 99%

“…Membranes were blocked with the blocking agent (BSA+ 0.05% Tween‐20) for overnight: then incubated with primary antibodies for 1 hr followed by a horseradish peroxidase‐conjugated secondary antibody (Anti‐rabbit IgG). Finally, protein detection was performed by Chemiluminescent HRP Substrate (Millipore) for 5 min and visualised on commercial x‐ray film (Pazoki‐Toroudi, Ajami, & Habibey, ; Pazoki‐Toroudi, Ajami, Babakoohi, et al., ).…”

Section: Methodsmentioning

confidence: 99%

FOXO1 targeting by capsaicin reduces tissue damage after testicular torsion

et al. 2018

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Testicular torsion-related oxidative stress causes a sequential chain of DNA damage, lipid peroxidation and cell death that leads to the derangement in the sperm functions and infertility. Capsaicin that has been applied for pain relief and cancer prevention possesses antioxidant properties which can be exploited to confer cell survival under ischaemic testis damage. Wistar male rats weighing 150-200 g were randomly divided into four groups: (i) sham group (all procedures except torsion of testis), (ii) ischaemia group (TT group), (iii) three TT groups treated with different dose of capsaicin (TT + different doses of Cap) and (iv) three control groups treated with different doses of capsaicin (100, 500 and 1000 ug/ml). Capsaicin administration significantly decreased the expression of pro-apoptotic factors and increased the expression of anti-apoptotic factors. Likewise, the expression of FOXO1 is significantly increased by higher doses of the capsaicin. Histological assessment by H&E and TUNEL method also exhibited an improved testicular morphology and decreased apoptosis in testes. These results suggested clinical potential for capsaicin in treatment of testicular torsion by targeting FOXO1 and apoptotic pathways.

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Effects of diphencyprone on expression of Bcl-2 protein in patients with Alopecia areata

Abstract: The results of this study indicate the positive effect of DPCP on regulation (inhibition) of apoptotic process in patients with AA.

Cited by 12 publications

References 12 publications

Rosmarinus Improved Skin Flap Survival Through mTOR Dependent Pathway

Rosmarinus Improved Skin Flap Survival Through mTOR Dependent Pathway

Gsdma3 gene is needed for the induction of apoptosis-driven catagen during mouse hair follicle cycle

FOXO1 targeting by capsaicin reduces tissue damage after testicular torsion

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