Effects of dimethylsulfoxide on behavior and antioxidant enzymes response of planarian Dugesia japonica

Abstract

In this study, the toxicity, behavioral and antioxidant activity effects of dimethylsulfoxide (DMSO) on planarian Dugesia japonica were investigated. The results showed that the mortality was directly proportional to the DMSO concentration, and planarian locomotor velocity decreased as the concentration of DMSO increased. The recovery of the motility for planarians pre-exposed to DMSO was found to be time- and dose-dependent, and only those pre-exposed to 0.1-3% DMSO resulted in full recovery. The antioxidant enzymes of planarians in response to long-term DMSO stress was also altered in a time- and dose-dependent manner. Planarians revealed more tolerance to DMSO toxicity at low DMSO (0.1%) level in short- and long-term DMSO stress, in which an efficient antioxidant system was involved and the motility was not affected.

Toxicity profiles and solvent–toxicant interference in the planarian Schmidtea mediterranea after dimethylsulfoxide (DMSO) exposure

“…In vertebrates, changes in motility were reported after acute DMSO exposures, ranging from a few minutes up to 2 h, in concentrations between 500 and 64 0000 µl l −1 DMSO (Castro et al , ; Sackerman et al , ). In previous studies on planarians, an exposure of 1 h to 5000–10 0000 µl l −1 DMSO produced significant, concentration‐dependent changes in the spontaneous locomotor velocity (pLMV), whereas an exposure of 8 min to 1000 µl l −1 DMSO did not cause any behavioural or toxic effects (Pagan et al , ; Yuan et al ., ). We also observed an increased motility of the organisms after an exposure of 1 day to low concentrations of DMSO (500 and 1000 µl l −1 ).…”

“…Planarians are considered as a model organism for neurotoxicological research (Best and Morita, 1991;Grebe and Schaeffer, 1991;Pagan et al, 2006;Raffa and Scott, 2008;Yuan et al, 2012;Tsushima et al, 2012;Raffa et al, 2013). They possess a well-organized nervous system, including many of the same neuroactive compounds as vertebrates and, together with their extensive repertoire of behaviour, they are suitable organisms for the study of neuronal effects of toxic substances.…”

“…They possess a well-organized nervous system, including many of the same neuroactive compounds as vertebrates and, together with their extensive repertoire of behaviour, they are suitable organisms for the study of neuronal effects of toxic substances. In planarians, an exposure to DMSO alters mobility and behaviour and affects the levels of anti-oxidants (Pagan et al, 2006;Yuan et al, 2012). High DMSO concentrations are mutagenic according to the Ames test, and as such, DMSO-induced oxidative stress may be a precursor of a carcinogenic response (Hakura et al, 1993).…”

The Improvement of The Endogenous Antioxidant Property of Stone Fish (Actinopyga lecanora) Tissue Using Enzymatic Proteolysis

“…The main component of stone fish tissue extract with the highest peak area of 59.7% identified by GC/MS was dimethyl sulfoxide, which is an amphiphilic compound with ability to dissolve lipophilic compounds [22]. Dimethyl sulfoxide was reported as a potential source of antioxidant in recent studies [22, 23]. …”

Planarian brain regeneration as a model system for developmental neurotoxicology

“…For example, several studies have analyzed how the activity of different biomarkers changes during the course of toxicant exposure (Yuan et al. ; Wu et al. ; Zhang et al.…”