Effects of dihydro-epi-deoxyarteannuin B on artemisinin biosynthesis, transcriptional profile and associated gene expression in suspension-cultured cells of Artemisia annua

Effects of UV-B and DMSO Treatments on the Production of Artemisinin in Low-Artemisinin Producing Artemisia annua Cell Cultures

“…The literature provided evidence that prolonged incubation periods might affect intracellular artemisinin accumulation and in culture medium. Highest artemisinin accumulation was recorded when elicited cell cultures were harvested after incubation periods ranging from 4 h to 5 days (Baldi and Dixit, 2008;Caretto et al, 2011;Durante et al, 2011;Salehi et al, 2019;Zhu et al, 2020). Caretto et al (2011) showed that cells treated with methyl jasmonate for prolonged periods (24 h, 48 h and 5 days) had artemisinin levels that dropped to values similar to control cells and were accompanied by a decline in CYP71AV1 expression.…”

“…The physiological state of cultures and the regulation of cellular metabolic homeostasis were identified as important factors that might influence stress responses of A. annua that in turn might result in a distortion of elicitation outcome (Kam and Yap, 2020). While Ferreira and Janick (1996b) also reported either trace or no artemisinin in HAP cell cultures, recent elicitation experiments on A. annua suspension cultures improved artemisinin production by several folds (Baldi and Dixit, 2008;Chan et al, 2010;Caretto et al, 2011;Durante et al, 2011;Salehi et al, 2019;Zhu et al, 2020). The discrepancy in elicitation successes may lie in the different time point selection as to when cells were harvested post-treatment.…”