Effects of Diffusion Limitations on the Response and Sensitivity of Biosensors

Baronas, Romas; Ivanauskas, Feliksas; Kulys, Juozas

doi:10.1007/978-3-030-65505-1_2

Cited by 1 publication

(5 citation statements)

References 68 publications

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“…x and y axes represent directions that are parallel and perpendicular to the pore in GDL, respectively. Two diffusion layers (GDL and LDL) describe the diffusion resistance of each species before it reaches the EL surface . The thickness of the diffusion layer is the same for each species and remains unchanged with time.…”

Section: Model Developmentmentioning

confidence: 99%

“…The thickness of the diffusion layer is the same for each species and remains unchanged with time. The diffusion layer should be taken into consideration in the model when the EL thickness (δ EL ) is thinner than about 25 times of LDL thickness (δ LDL ) or GDL thickness (δ GDL ) to reduce the prediction deviation of the model . The GDL is modeled as the gas physical field (Ω g ).…”

Section: Model Developmentmentioning

confidence: 99%

“…Two diffusion layers (GDL and LDL) describe the diffusion resistance of each species before it reaches the EL surface. 22 The thickness of the diffusion layer is the same for each species and remains unchanged with time. The diffusion layer should be taken into consideration in the model when the EL thickness (δ EL ) is thinner than about 25 times of LDL thickness (δ LDL ) or GDL thickness (δ GDL ) to reduce the prediction deviation of the model.…”

Section: ■ Model Developmentmentioning

confidence: 99%

“…The diffusion layer should be taken into consideration in the model when the EL thickness (δ EL ) is thinner than about 25 times of LDL thickness (δ LDL ) or GDL thickness (δ GDL ) to reduce the prediction deviation of the model. 22 The GDL is modeled as the gas physical field (Ω g ). The EL and LDL are modeled as the liquid physical field (Ω l ).…”

Section: ■ Model Developmentmentioning

confidence: 99%

“…Mathematical modeling is an effective and promising method for the investigation of transport phenomena , and reaction kinetics. − In this work, we report a novel mathematical model for a three-phase oxidase enzymatic reaction system based on glucose oxidase (GOx), a model enzyme. A steady-state isothermal 2D model was developed to describe interphase (gas dissolution) and intraphase (species diffusion) mass transfer and enzyme catalysis reaction.…”

Section: Introductionmentioning

confidence: 99%

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Mathematical Model for a Three-Phase Enzymatic Reaction System

Zou

Wang

Xiao

et al. 2023

Ind. Eng. Chem. Res.

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The enzymatic reaction system with a solid−liquid−gas three-phase interface microenvironment allows oxygen to be directly supplied to the oxidase catalytic reaction from the gas phase, effectively improving enzyme kinetics as compared with the conventional two-phase system. For this new system, a mathematical model is developed in this work to describe the enzymatic reaction coupled with interphase mass transfer, by which the influences of three-phase interfacial microenvironment on reaction kinetics can be systematically and quantitatively explored. The numerical simulations reveal that the flux of oxygen transport across the interface between the gas phase and the enzyme layer dominantly determines the H 2 O 2 production rate. The porous substrate possessing larger porosity and smaller pores, when coated with a thick and concentrated enzyme layer, can potentially lead to higher oxygen supply and hence a higher H 2 O 2 production rate. Moreover, regardless of the pore diameter, the H 2 O 2 production rate remains constant after the porosity is greater than 0.8, and if the enzyme concentration is not less than 5 mol m −3 , the H 2 O 2 production rate no longer changes after the thickness of the enzyme layer is greater than 0.5 μm. This work offers a powerful in silico tool for the investigation of the three-phase enzymatic reaction system. The quantitative results and mechanistic findings will lead to optimized design of this promising system.

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Section: Model Developmentmentioning

confidence: 99%

Section: Model Developmentmentioning

confidence: 99%

Section: ■ Model Developmentmentioning

confidence: 99%

Section: ■ Model Developmentmentioning

confidence: 99%