Effects of different voltages and pulse durations on caprine tetraploid embryo production

Pathak, Juhi; Kharche, S. D.; Goel, Anjana; Sikarwar, A.K.S.; Ranjan, Ravi; Chauhan, M. S.

doi:10.5958/0973-9718.2020.00004.5

Indi. Jour. of Small Rumin.

2020

DOI: 10.5958/0973-9718.2020.00004.5

|View full text |Cite

Effects of different voltages and pulse durations on caprine tetraploid embryo production

Juhi Pathak¹,

S. D. Kharche²,

Anjana Goel³

et al.

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...

Citation Types

Supporting

Mentioning

Contrasting

Year Published

2022

Publication Types

Select...

Article1

Relationship

Self Cite0

Independent1

Authors

Journals

Cited by 1 publication

(1 citation statement)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…A total of 644 cleaved two-cell caprine IVF embryos were subjected to electrofusion at 1.2 kV/cm for 4 µs to produce tetraploid embryos (Pathak et al 2020). The percentage of fusion recorded was 91.44±1.22% and fused embryos were further developed upto 4 cell stage as 4 cell embryos formed were utilized for chimeric embryo production.…”

mentioning

confidence: 99%

Effect of hypoxia on production of caprine chimeric blastocyst

Kharche

Pathak

Sikarwar³

et al. 2022

Indian J of Anim Sci

View full text Add to dashboard Cite

This study was conducted to evaluate the effect of 5% and 21% oxygen concentrations on chimeric embryo development. Inner cell mass (ICM) from parthenogenetic activated blastocyst were used to produce ES cell-like cells. A pair of zona-free tetraploid embryos and one clump of pESCs at passage 2 and 3 were aggregated on granulosa monolayer in such a manner that the clump of pESCs was sandwiched between the tetraploid embryos. The aggregates prepared were randomly divided into two groups viz. Group 1 (21% O2) (n=54) and Group 2 (5% O2) (n=50) followed by culture in humidified atmosphere of 5% CO2 at 38.5°C in a CO2 incubator. Aggregation rate and embryo quality was similar in both the groups. The percentage of aggregation, 8-16 cell, morula and blastocyst Group 1 (21% O2) was 91.66±4.04%, 49.07±5.08%, 25.92±6.05% and 16.66±4.95%, respectively while the percentage of aggregation, 8-16 cell, morula and blastocyst Group 2 (5% O2) was 96.59±2.83%, 51.89±9.14%, 16.28±5.52% and 28.40±8.10%, respectively. The aggregates and 8-16 cell stage embryos formed in Group 2 (5% O2) were comparatively higher than in Group 1 (21% O2) while morula formation in Group 2 (5% O2) was comparatively lower than in Group 1 (21% O2). Also, Group 2 (5% O2) had significantly more blastocysts as compared to Group 1 (21% O2). To conclude, culture under 5% O2 leads to improved aggregation and also enhances the production of blastocysts rather than with atmospheric oxygen concentrations (21%) during embryo incubation.

show abstract

mentioning

confidence: 99%

Effect of hypoxia on production of caprine chimeric blastocyst

Kharche

Pathak

Sikarwar³

et al. 2022

Indian J of Anim Sci

View full text Add to dashboard Cite

show abstract

scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.

Contact Info

hi@scite.ai

10624 S. Eastern Ave., Ste. A-614

Henderson, NV 89052, USA

Blog Terms and Conditions API Terms Privacy Policy Contact Cookie Preferences Do Not Sell or Share My Personal Information

Made with 💙 for researchers

Part of the Research Solutions Family.

Effects of different voltages and pulse durations on caprine tetraploid embryo production

Cited by 1 publication

References 0 publications

Effect of hypoxia on production of caprine chimeric blastocyst

Effect of hypoxia on production of caprine chimeric blastocyst

Contact Info

Product

Resources

About