Effects of Different Times of Glutaraldehyde 2% on Bacillus subtilis Spores (In Vitro)

Lasemi, Eshagh; Motamedi, Mohammad Hossain Kalantar; Navi, Fina; Rezae, Maryam; Nikfar, Niousha Homay; Danial, Zahra; Azizpour, Roojan

doi:10.15171/hpr.2017.28

Cited by 4 publications

(2 citation statements)

References 11 publications

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“…The studies by Lasemi et al (2017) demonstrated the impact of 2% solution of glutaraldehyde on the spores of B. subtilis. The results showed that 102 colonies were present on the 10th minute, 18.6 ± 3.4 on the 15th minute, 6.2 ± 1.4 on the 20th minute, 2.1 ± 0.8 on the 25th minute and no colonies after 30 minutes.…”

Section: Discussionmentioning

confidence: 99%

“…LD 50 for benzaldehyde -142 ± 64 mg/l (Boyko & Brygadyrenko, 2017b). The literature contains a large amount of data on the impact of alkylbenzyldimethylammonium chloride, didecyldimethylammonium chloride, formaldehyde, glutaraldehyde and other certain substances on microorganisms (Braoudaki et al, 2005;Blondeau et al, 2007;Fazlara & Ekhtelat, 2012;Vaerewijck et al, 2012;Ivancovic et al, 2013;Lasemi, 2017). Therefore, the objective of our study was to perform a comparative assessment of bactericidal, protistocidal and nematocidal properties of mixtures of alkylbenzyldimethylammonium chloride, didecyldimethylammonium chloride and glutaraldehyde; alkylbenzyldimethylammonium chloride, formaldehyde and glutaraldehyde; sodium dodecyl sulfate (SDS), oleum terebinthini and glutaraldehyde, and also formaldehyde and glutaraldehyde.…”

Section: Introductionmentioning

confidence: 99%

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Bactericidal, protistocidal and nematodicidal properties of mixtures of alkyldimethylbenzyl ammonium chloride, didecyldimethyl ammonium chloride, glutaraldehyde and formaldehyde

Zazharskyi

Davydenko

Kulishenko

et al. 2018

Regul. Mech. Biosyst.

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We conducted a comparative analysis of the impact of disinfecting preparations on the cryogenic stains of microorganisms, and also on Haemonchus contortus (Rudolphi 1803), invasive larvae of the ruminants. To test the preparations for disinfection, we used laboratory analyses with methods of biotesting, particularly with the use of Paramecium caudatum Her., Tetrahymena pyriformis Ehrenberg. We researched mixtures of substances: alkylbenzyldimethylammonium chloride (C24H42IN, BAK, mixture of homologues alkylbenzyldimethylammonium chloride and with n-C12H25, n-C14H29 and n-C16H33), didecyldimethylammonium Chloride (DDAC, C22H48ClN) and glutaraldehyde (C5H8O2); formaldehyde (CH2O), alkylbenzyldimethylammonium chloride and glutaraldehyde in 1% have bactericidal properties for the following cryogenic strains of microorganisms: Staphylococcus аureus, Salmonella typhimurium, Escherichia coli, Listeria monocytogenes, Proteus vulgaris, Serracia marcescens, Pseudomonas аeruginosa, Enterococcus faecalis and Yersinia enterocolitica. The Bacillus сereus were affected by the preparations bacteriostatically: we observed growth in the colonies in the medium with addition of 1% solution of mixture of alkylbenzyldimethylammonium chloride and didecyldimethylammonium chloride, and also 1%, 5% and 10% of solution of mixture of glutaraldehyde, formaldehyde and alkylbenzyldimethylammonium chloride. Also, these mixtures of substances have nematocidal properties. Death of 100% of L3 H. contortus after 24 hour exposure was observed with use of 1% solution of mixture of alkylbenzyldimethylammonium chloride and didecyldimethylammonium chloride, and also 5% glutaraldehyde, formaldehyde and alkylbenzyldimethylammonium chloride. Effective disinfection measures perform a leading role in providing stable veterinary well-being of livestock and healthcare of the population. Maximum toxicity during usage of the mixtures on P. caudatum was observed for the mixture of alkylbenzyldimethylammonium chloride and didecyldimethylammonium chloride, and also for formaldehyde and glutaraldehyde. The lowest toxicity for T. pyriformis was observed with use of the mixture of glutaraldehyde, sodium dodecylsulfate (SDS) and oleum terebinthini, and also the mixture of formaldehyde and glutaraldehyde, the highest formaldehyde and alkylbenzyldimethylammonium chloride. Thus, the most promising mixtures for use in veterinary medicine were determined to the following: alkylbenzyldimethylammonium chloride, didecyldimethylammonium chloride and glutaraldehyde, and also formaldehyde, alkylbenzyldimethylammonium chloride and glutaraldehyde.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Bactericidal, protistocidal and nematodicidal properties of mixtures of alkyldimethylbenzyl ammonium chloride, didecyldimethyl ammonium chloride, glutaraldehyde and formaldehyde

Zazharskyi

Davydenko

Kulishenko

et al. 2018

Regul. Mech. Biosyst.

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Preparation of calcium phosphate ion clusters through atomization method for biomimetic mineralization of enamel

Dong,

Qiu,

Zhu

et al. 2024

J Biomedical Materials Res

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Dental enamel is a mineralized extracellular matrix, and enamel defect is a common oral disease. However, the self‐repair capacity of enamel is limited due to the absence of cellular components and organic matter. Efficacy of biomimetic enamel mineralization using calcium phosphate ion clusters (CPICs), is an effective method to compensate for the limited self‐healing ability of fully developed enamel. Preparing and stabilizing CPICs presents a significant challenge, as the addition of certain stabilizers can diminish the mechanical properties or biosafety of mineralized enamel. To efficiently and safely repair enamel damage, this study quickly prepared CPICs without stabilizers using the atomization method. The formed CPICs were evenly distributed on the enamel surface, prompting directional growth and transformation of hydroxyapatite (HA) crystals. The study revealed that the mended enamel displayed comparable morphology, chemical composition, hardness, and mechanical properties to those of the original enamel. The approach of repairing dental enamel by utilizing ultrasonic nebulization of CPICs is highly efficient and safe, therefore indicating great promise.

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Characterization of individual spores of two biological insecticides, Bacillus thuringiensis and Lysinibacillus sphaericus, in response to glutaraldehyde using single-cell optical approaches

Chen,

Wang,

et al. 2024

Arch Microbiol

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Effects of Different Times of Glutaraldehyde 2% on Bacillus subtilis Spores (In Vitro)

Cited by 4 publications

References 11 publications

Bactericidal, protistocidal and nematodicidal properties of mixtures of alkyldimethylbenzyl ammonium chloride, didecyldimethyl ammonium chloride, glutaraldehyde and formaldehyde

Bactericidal, protistocidal and nematodicidal properties of mixtures of alkyldimethylbenzyl ammonium chloride, didecyldimethyl ammonium chloride, glutaraldehyde and formaldehyde

Preparation of calcium phosphate ion clusters through atomization method for biomimetic mineralization of enamel

Characterization of individual spores of two biological insecticides, Bacillus thuringiensis and Lysinibacillus sphaericus, in response to glutaraldehyde using single-cell optical approaches

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