Effects of different swine manure to wheat straw ratios on antibiotic resistance genes and the microbial community structure during anaerobic digestion

Song, Wen; Wang, Xiaojuan; Gu, Jie; Zhang, Sheqi; Yin, Yanan; Li, Yang; Qian, Xiaoqing; Sun, Wei

doi:10.1016/j.biortech.2017.01.054

Cited by 124 publications

(24 citation statements)

References 40 publications

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“…In this study, the CCA results showed that the abundance of Gemmatimonadetes, Actinobacteria, Proteobacteria, and Firmicutes was positively correlated with the abundance of ARGs (tetO, tetQ, and tetW) during the AC and AD processes ( Supplementary Table S3). Song et al (2017) suggested that Bacteroidetes and Spirochetes are responsible for the variation in TC-associated ARGs (tetC, tetW, and tetQ) after the AD process. In our study, CCA1 and CCA2 accounted for 47.83% of the total variation in the bacterial communities ( Figure 5A).…”

Section: Discussionmentioning

confidence: 99%

“…Zhang et al (2006) indicated that composting is a new alternative bioremediation technology for the treatment of antibiotics. Song et al (2017) also demonstrated that bacterial communities accounted for 46.7% of the total variation in the overall ARG profiles during the AD process. In addition, the physicochemical properties during manure treatment also contribute to the efficiencies of antibiotic degradation by modulating microbial community structures.…”

Section: Introductionmentioning

confidence: 86%

“…In addition, the physicochemical properties during manure treatment also contribute to the efficiencies of antibiotic degradation by modulating microbial community structures. Song et al (2017) indicated that these physicochemical properties [soluble chemical oxygen demand) SCOD, pH, NH 4 + , and volatile fatty acids (VFAs)] accounted for 30.8% of the total variation in the overall ARG profiles during the AD process. In this study, we used swine manure, which is rich in four tetracycline antibiotics (TCs), as a raw material to compare the performance of AC and AD processes in terms of antibiotic degradation efficiency and accumulation rate of TC-associated ARGs.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Differences in Tetracycline Antibiotic Resistance Genes and Microbial Community Structure During Aerobic Composting and Anaerobic Digestion

Luo

Zhang

et al. 2020

Front. Microbiol.

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Antibiotics are widely added to swine forage and are the main reason for the environmental accumulation of antibiotic resistance genes (ARGs) in swine manuredwelling microorganisms. Aerobic composting (AC) and anaerobic digestion (AD) are efficient methods for converting swine manure to bio-fertilizer while degrading residual antibiotics. However, the influence of these methods on ARG accumulation and the difference in their efficiency have rarely been investigated. In this study, we explored the variations in four tetracycline antibiotics (TCs) and their associated ARGs and in microbial communities after AC and AD treatment. After full-scale manure AC and AD, the four TCs were removed effectively. AD had a higher TC removal efficiency than AC and a slower rate of TC-associated ARG accumulation. In addition, the community structure was more stable in the AC and AD manures than in untreated manure, and the relationship among microbial species also evolved into competition from mutualism after both AC and AD treatment. It was also speculated that the genera Acholeplasma and Arthrobacter were the possible hosts of tetO, tetW, and tetQ; the shift in the prokaryotic community composition and the alleviation of selective pressure by TC degradation led to decreased relative abundance of ARGs in AD-and AC-treated manure.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 86%

Section: Introductionmentioning

confidence: 99%

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Differences in Tetracycline Antibiotic Resistance Genes and Microbial Community Structure During Aerobic Composting and Anaerobic Digestion

Luo

Zhang

et al. 2020

Front. Microbiol.

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“…TC can linger in edatope for over 1 year, which is relatively longer than other antibiotics. The long persistence of the TC pollutant could cause various negative environmental effects, like reducing the diversity of indigenous microbial communities [4,5], enriching TC-resistant microbes, and damaging functional microbiota [6]. Additionally, the long presence of antibiotics will cause dissemination of the corresponding antibiotic resistant genes (ARGs), and human pathogenic microbes may acquire antibiotic resistance via mobile gene elements (MGEs)-mediated horizontal gene transfer, subsequently threatening human health.…”

Section: Introductionmentioning

confidence: 99%

Construction of a Tetracycline Degrading Bacterial Consortium and Its Application Evaluation in Laboratory-Scale Soil Remediation

et al. 2020

Microorganisms

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As an environmental pollutant, tetracycline (TC) can persist in the soil for years and damage the ecosystem. So far, many methods have been developed to handle the TC contamination. Microbial remediation, which involves the use of microbes to biodegrade the pollutant, is considered cost-efficient and more suitable for practical application in soil. This study isolated several strains from TC-contaminated soil and constructed a TC-degrading bacterial consortium containing Raoultella sp. XY-1 and Pandoraea sp. XY-2, which exhibited better growth and improved TC degradation efficiency compared with single strain (81.72% TC was biodegraded within 12 days in Lysogeny broth (LB) medium). Subsequently, lab-scale soil remediation was conducted to evaluate its effectiveness in different soils and the environmental effects it brought. Results indicated that the most efficient TC degradation was recorded at 30 °C and in soil sample Y which had relatively low initial TC concentration (around 35 mg/kg): TC concentration decreased by 43.72% within 65 days. Soil properties were affected, for instance, at 30 °C, the pH value of soil sample Y increased to near neutral, and soil moisture content (SMC) of both soils declined. Analysis of bacterial communities at the phylum level showed that Proteobacteria, Bacteroidetes, Acidobacteria, and Chloroflexi were the four dominant phyla, and the relative abundance of Proteobacteria significantly increased in both soils after bioremediation. Further analysis of bacterial communities at the genus level revealed that Raoultella sp. XY-1 successfully proliferated in soil, while Pandoraea sp. XY-2 was undetectable. Moreover, bacteria associated with nitrogen cycling, biodegradation of organic pollutants, soil biochemical reactions, and plant growth were affected, causing the decline in soil bacterial diversity. Variations in the relative abundance of tetracycline resistance genes (TRGs) and mobile gene elements (MGEs) were investigated, the results obtained indicated that tetD, tetG, tetX, intI1, tnpA-04, and tnpA-05 had higher relative abundance in original soils, and the relative abundance of most TRGs and MGEs declined after the microbial remediation. Network analysis indicated that tnpA may dominate the transfer of TRGs, and Massilia, Alkanibacter, Rhizomicrobium, Xanthomonadales, Acidobacteriaceae, and Xanthomonadaceae were possible hosts of TRGs or MGEs. This study comprehensively evaluated the effectiveness and the ecological effects of the TC-degrading bacterial consortium in soil environment.

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“…Of them, anaerobic digestion not only degrades organic matter and removes pathogens effectively (3,4) but also produces renewable energy economically (5,6). Recent studies have begun to investigate the fate of ARGs during anaerobic digestion of manure (7)(8)(9). In anaerobic digestion, temperature is a critical variable which can affect the composition and function of the microbial community (10).…”

mentioning

confidence: 99%

Higher Temperatures Do Not Always Achieve Better Antibiotic Resistance Gene Removal in Anaerobic Digestion of Swine Manure

Huang

Zheng

Tian

et al. 2019

Appl Environ Microbiol

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This study employed high-throughput quantitative PCR and 16S rRNA sequencing to evaluate the effect of temperature and residual antibiotics on the dynamics of antibiotic resistance genes (ARGs) and microbial communities during anaerobic digestion of swine manure. The abundances of total ARGs and 16S rRNA genes significantly decreased in all of four treatments (25°C, 37°C, and 37°C with 50 mg of wet weight antibiotics of body weight, and 55°C). The abundances of most ARG types were significantly correlated with those of the 16S rRNA gene and transposase gene (P < 0.01). However, the abundances of total ARGs at 55°C were much higher than those of other treatments. Meanwhile, the microbial communities at 55°C, where the Streptococcus pathogen remained at a relatively high abundance and cellulose degraders and hydrogen producers, such as Ethanoligenens and Coprococcus bacteria, increased, were markedly different from those of other treatments. Redundancy analysis indicates that temperature, pH, and the genus Streptococcus had the highest explanation for ARG variation among experimental factors, chemical properties, and representative genera, respectively. Network analysis further showed that the genus Streptococcus contributed greatly to the higher ARG abundance at 55°C. The moderate antibiotic residue only caused a slight and transitory inhibition for microbially diverse populations and promotion for ARG abundance, probably due to the degradation of antibiotics and microbial adaptability. Our results clarify the cooperativity of gene transfer-related items on ARG variation and intensively prove that higher temperature cannot always achieve better ARG removal in anaerobic digestion unless pathogens and gene transfer elements are more efficiently inhibited. IMPORTANCE Antibiotic resistance genes (ARGs) are frequently detected with high abundance in manure-applied soils. Anaerobic digestion is one of widely used processes for animal waste treatment. Thus, it is critical to understand the potential of anaerobic digestion to attenuate ARGs. Although some previous studies recommended thermophilic digestion for ARG removal, they did not get sufficient evidence to support this view. The antibiotics applied to animals are mostly excreted through feces and urine because of incomplete metabolism. It is indispensable to know whether residual antibiotics in manure will hinder ARG attenuation in anaerobic digesters. The significance of our research is in comprehensively understanding the evolution and mechanism of ARGs in anaerobic digestion of swine manure affected by temperature and residual antibiotics, which will allow the development of an ARG elimination strategy before their release into the environment.

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Effects of different swine manure to wheat straw ratios on antibiotic resistance genes and the microbial community structure during anaerobic digestion

Cited by 124 publications

References 40 publications

Differences in Tetracycline Antibiotic Resistance Genes and Microbial Community Structure During Aerobic Composting and Anaerobic Digestion

Differences in Tetracycline Antibiotic Resistance Genes and Microbial Community Structure During Aerobic Composting and Anaerobic Digestion

Construction of a Tetracycline Degrading Bacterial Consortium and Its Application Evaluation in Laboratory-Scale Soil Remediation

Higher Temperatures Do Not Always Achieve Better Antibiotic Resistance Gene Removal in Anaerobic Digestion of Swine Manure

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