Effects of different spacer arms on Cibacron Blue modification and protein affinity adsorption on magnetic microspheres

Zhang, Dong‐Hao; Chen, Na; Yang, Mei-Na; Dou, Yi-Fang; Sun, Jie; Liu, Yadong; Zhi, Gao‐Ying

doi:10.1016/j.molcatb.2016.08.007

Cited by 14 publications

(30 citation statements)

References 32 publications

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“…Encouragingly, BSA adsorption on CB‐PEI 600‐PMMA, CB‐PEI 1800‐PMMA, and CB‐PEI 70000‐PMMA microspheres, respectively, reached 95.0, 86.8, and 86.1 mg BSA g ‐1 microspheres at 1.5 mg mL ‐1 . Our recent results showed that CB‐EDA‐PMMA magnetic microspheres exhibited a BSA adsorption capacity of 69.2 mg g ‐1 at 1 mg mL ‐1 of BSA initial concentration . In comparison, the CB‐PEI‐PMMA magnetic microspheres provided higher BSA adsorption of about 85 mg g ‐1 at 1 mg mL ‐1 , indicating that CB‐PEI‐PMMA microspheres possessed excellent adsorption capability.…”

Section: Resultsmentioning

confidence: 94%

“…First, the thermodynamic motion of BSA increased with increase of temperature, which was beneficial to desorption of BSA from the carrier. Second, BSA conformation suffered, to some extent, from structural rearrangement due to temperature enhancement, which affected the interaction between BSA and microspheres . Third, the hydrogen bond interaction and intermolecular Van der waals forces between BSA and PMMA microsphere would decrease with increase of temperature, resulting in the obvious decline of BSA adsorption capacity at temperatures above 35 °C.…”

Section: Resultsmentioning

confidence: 99%

“…The PMMA magnetic microspheres were prepared via a modified suspension polymerization method in the presence of oleic acid‐coated magnetic particles . Briefly, 90 mg of oleic acid coated magnetic particles was mixed with 3.5 mL of methylmethacrylate (MMA) and 250 µL of divinylbenzene (DVB).…”

Section: Methodsmentioning

confidence: 99%

“…Briefly, 20 mg of microspheres were mixed with 2 mL of 1.5 mg mL ‐1 BSA buffer solution (Na 2 HPO 4 ‐NaH 2 PO 4 buffer, pH 7.0) in a shaking incubator at 35 °C at 110 rpm for 4 h. After adsorption, magnetic microspheres were separated by a magnet. The BSA adsorption capacity of the magnetic microspheres was determined by measuring the initial and final concentration of BSA in the solution using Coomassie Brilliant Blue …”

Section: Methodsmentioning

confidence: 99%

“…Cibacron Blue F3GA (CB), one of the triazine dyes, is often used as affinity ligand to adsorb protein in protein separation and enzyme immobilization . It is known that CB interacts strongly and reversibly with protein by the combination of hydrophobic, hydrogen bonding and electrostatic interactions . Moreover, CB can be easily immobilized onto the hydroxyl or amino group bearing supports by the nucleophilic substitution reaction.…”

Section: Introductionmentioning

confidence: 99%

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Polyethyleneimine grafting and Cibacron Blue F3GA modifying poly(methylmethacrylate) magnetic microspheres for protein adsorption

Dou

Zhang

Liu

et al. 2017

J of Chemical Tech & Biotech

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BACKGROUND Polyethyleneimine (PEI) has attracted wide interest due to not only its good hydrophilicity but also its high density of amino groups across the polymer chain. In order to study in depth the effect of PEI as spacer arm on dye ligand modification and further protein adsorption, poly(methylmethacrylate) (PMMA) magnetic microspheres were fabricated, grafted with PEI, and further modified with an affinity dye‐ligand, Cibacron Blue F3GA (CB). RESULTS CB density of these CB‐PEI‐PMMA microspheres was determined to be 370.7 µmol g‐1 for CB‐PEI 600‐PMMA, 355.2 µmol g‐1 for CB‐PEI 1800‐PMMA, and 317.6 µmol g‐1 for CB‐PEI 70000‐PMMA microspheres, respectively. Moreover, bovine serum albumin (BSA) adsorption capacity of PEI‐PMMA and CB‐PEI‐PMMA microspheres increased greatly compared with PMMA microspheres. The effects of temperature, BSA initial concentration, pH and ionic strength on BSA adsorption were studied. CB‐PEI 70000‐PMMA microspheres exhibited the maximum adsorption of 103.6 mg g‐1. CONCLUSION The results demonstrated the versatility of PEI as spacer arm. The information obtained from the present work could be utilized for rational design of carriers for protein adsorption or enzyme immobilization. © 2017 Society of Chemical Industry

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Section: Resultsmentioning

confidence: 94%

Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Polyethyleneimine grafting and Cibacron Blue F3GA modifying poly(methylmethacrylate) magnetic microspheres for protein adsorption

Dou

Zhang

Liu

et al. 2017

J of Chemical Tech & Biotech

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An efficient protein immobilization strategy: protein encapsulated in nano molecular cages

Xia

Dou

Pei

et al. 2019

J of Chemical Tech & Biotech

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BACKGROUND: To immobilize protein firmly and maintain its native conformation, a 'nano molecular cage' was designed to encapsulate bovine serum albumin (BSA) on the surface of poly(methyl methacrylate) (PMMA) magnetic microspheres by cross-linking poly(ethylene imine) (PEI) chains. RESULTS:The amount of BSA adsorbed on PMMA@PEI microspheres decreased significantly from 70.6 to 7.6 mg g −1 microsphere after desorption under high ionic strength. A similar phenomenon could also be observed for BSA adsorbed on PMMA@PEI-CB (Cibacron Blue F3GA) microspheres. In contrast, the amount of BSA encapsulated in molecular cages only decreased a little after desorption. Furthermore, conformational analysis by Fourier transform infrared spectroscopy (FTIR) showed that the contents of -helices and -sheets decreased slightly by 3.3 and 0.3% respectively on encapsulating BSA in molecular cages. Furthermore, the effects of various factors, including glutaraldehyde amount, cross-linking time and temperature, on BSA encapsulation were investigated. CONCLUSION:The results demonstrated that molecular cages could not only immobilize protein firmly but also maintain its native conformation. Therefore molecular cage encapsulation is suggested to be a potentially valuable protocol in enzyme immobilization. Attachment of CB onto magnetic microspheresCB was attached onto PMMA@PEI magnetic microspheres by nucleophilic reactions between the amino group of PEI and the chloro group of CB. Briefly, 20 mg of PMMA@PEI magnetic microspheres were added into 2 mL of CB solution (3 mg mL −1 ) in a shaker at 160 rpm and 50 ∘ C. To deposit the dye on the microspheres, 140 mg of NaCl was added into the mixture. After 30 min, 100 mg of Na 2 CO 3 was added to speed up the reaction. The reaction was carried out for 4 h. Thereafter, the resulting PMMA@PEI-CB magnetic microspheres were washed with 950 mL L −1 alcohol and distilled water several times until no CB was detected in the supernatant.

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Chitosan‐based nanocomposite membranes with improved properties: Effect of cellulose acetate blending and TiO₂ nanoparticles incorporation

et al. 2017

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The aim of this work was to synthesize nanocomposite membranes based on chitosan (CS) and cellulose acetate (CA) blends containing titanium dioxide (TiO2) nanoparticles and investigate their morphological, structural, mechanical, and thermal properties along with dye affinity ligand content for using potentially as bioadsorption applications. The samples were characterized by means of scanning electronic microscopy (SEM), energy dispersive X‐ray (EDX), Fourier transform infrared spectroscopy (FTIR), tensile, thermogravimetric analysis (TGA), differential scanning calorimetry (DSC), and water contact angle (WCA). The SEM micrograph images showed a uniform and microporous morphology of the prepared membranes. FTIR analysis confirmed the presence of CA and TiO2 nanoparticles through the CS matrix in which the components used had good compatibility. The mechanical and thermal studies emphasized that strong interactions occurred between CS, CA, and TiO2 nanoparticles. Blending CS with CA and the addition of TiO2 nanoparticles enhanced the hydrophilic property of CS membranes, significantly. The CS/CA based nanocomposite containing 1.5 wt% of TiO2 nanoparticles showed the remarkable improvements in ultimate tensile strength values and an increase in stiffness more than three times compared to the neat CS membrane sample. Finally, considering the results of tensile test and immobilized Cibacron Blue F3G‐A (CB) content measurement, the nanocomposite samples denoted by (50/50) CS/CA/TiO2 (1.5%) were nominated as the promising material used for potentially bioadsorptive membrane. POLYM. COMPOS., 39:4452–4466, 2018. © 2017 Society of Plastics Engineers

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Effects of different spacer arms on Cibacron Blue modification and protein affinity adsorption on magnetic microspheres

Cited by 14 publications

References 32 publications

Polyethyleneimine grafting and Cibacron Blue F3GA modifying poly(methylmethacrylate) magnetic microspheres for protein adsorption

Polyethyleneimine grafting and Cibacron Blue F3GA modifying poly(methylmethacrylate) magnetic microspheres for protein adsorption

An efficient protein immobilization strategy: protein encapsulated in nano molecular cages

Chitosan‐based nanocomposite membranes with improved properties: Effect of cellulose acetate blending and TiO₂ nanoparticles incorporation

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Effects of different spacer arms on Cibacron Blue modification and protein affinity adsorption on magnetic microspheres

Cited by 14 publications

References 32 publications

Polyethyleneimine grafting and Cibacron Blue F3GA modifying poly(methylmethacrylate) magnetic microspheres for protein adsorption

Polyethyleneimine grafting and Cibacron Blue F3GA modifying poly(methylmethacrylate) magnetic microspheres for protein adsorption

An efficient protein immobilization strategy: protein encapsulated in nano molecular cages

Chitosan‐based nanocomposite membranes with improved properties: Effect of cellulose acetate blending and TiO2 nanoparticles incorporation

Contact Info

Product

Resources

About

Chitosan‐based nanocomposite membranes with improved properties: Effect of cellulose acetate blending and TiO₂ nanoparticles incorporation