Effects of different processing methods on five main chemical constituents of Anemarrhena asphodeloides Bge. studied by high performance liquid chromatography

Abstract

A high performance liquid chromatographic (HPLC) method has been developed for the simultaneous determination of neomangiferin, mangiferin, timosaponin B III, anemarrhenasaponin I and timosaponin A III in the products of Anemarrhena asphodeloides Bge. processed by different methods. By comparing and analyzing the variation of the contents of the five components, the effects of processing on chemical constituents of Anemarrhena asphodeloides Bge. were explored, which provided evidences for the relevance between processing and the property changes of Anemarrhena asphodeloides Bge. The chromatographic separation was performed on an Alltima C18 column (250 mm x 4.6 mm, 5 microm) with a gradient elution of acetonitrile (A) and 0. 1% formic acid (B) (0 - 10 min, 5% A - 20% A; 10 - 15 min, 20% A - 25% A; 15 -30 min, 25% A - 80% A; 30 -35 min, 80% A - 100% A) at a flow rate of 0.8 mL/min. Neomangiferin and mangiferin were detected by an ultraviolet detector at 265 nm and room temperature. Timosaponin B III, anemarrhenasaponin I and timosaponin A III were detected by an evaporative light scattering detector with the drift temperature at 50 degrees C and gas pressure at 179.1 kPa (26 psi). To some extent, the contents of the major components varied in different processed products of Anemarrhena asphodeloides Bge. The results indicated that different processing methods caused significant differences in the contents of the major components of Anemarrhena asphodeloides Bge. It is of great use for further researching the relevance of the processing methods to pharmacodynamics of Anemarrhena asphodeloides Bge.

Investigation of pharmacodynamic material basis of Anemarrhenae Rhizoma and its processed products based on plant metabolomics and molecular docking technology

“…In recent studies, the chemical compositions of SAR were found to markedly different from those of its crude materials. But the differential chemical components before and after processing were explained separately from the type or content 8–10 . Previous studies focused on the changes in chemical composition and efficacy of different processed products of AR.…”

“…But the differential chemical components before and after processing were explained separately from the type or content. [8][9][10] Previous studies focused on the changes in chemical composition and efficacy of different processed products of AR. However, it was difficult to find a corresponding relationship between the differential components and effects.…”

A novel ultra high‐performance liquid chromatography‐tandem mass spectrometry method for the simultaneous determination of xanthones and steroidal saponins in crude and salt‐processed Anemarrhenae Rhizoma aqueous extracts

“…However, crude herbs should be properly processed before it can be used for their specified applications in clinic. The processing of AR has a long history and various excipients, such as rice wine, salt solution, and wheat bran, which have been traditionally used in different areas of China . Among these, salt processing by stir heating with salt solution is the most widely used method.…”