Effects of different nitrogen sources on the production of Hyaluronic acid by Streptococcus

Pan, Nicole Caldas; Marques, Renan Cantanti; Pereira, Hanny Cristina Braga; Vignoli, Josiane Alessandra; Celligoi, Maria Antonia Pedrine Colabone

doi:10.1186/1753-6561-8-s4-p204

Cited by 2 publications

(2 citation statements)

References 3 publications

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“…The product yield coefficient (YP/S) and product yield per biomass coefficient (YP/S) was higher with nutrient rich media containing 50 g/L glucose. The productivity is also higher in nutrient rich media, with a value of 0.028 g HA/L/h which is in accordance to results obtained by other researchers which suggests that presence of nitrogen source increases the production of HA (Pan et al, 2014).…”

Section: Effect Of Nutrient Rich Media On Ha Fermentation By Recombinsupporting

confidence: 91%

Cloning and expression of hyaluronan synthase (hasA) in recombinant Escherichia coli BL21 and its hyaluronic acid production in shake flask culture

Lai¹,

Teo²

2019

MJM

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Aims: Hyaluronic acid (HA) is a high molecular weight polymer and a major component of mucoid capsule in bacteria and extracellular matrix (ECM) of vertebrate tissue. Due to its unique characteristics, HA is used extensively in medical and cosmetic field. However, because of the exotoxins production from animal tissues extraction and Streptococcus zooepidemicus, HA production by recombinant microorganisms has gained interest. The present study was aimed at cloning of hasA gene in Escherichia coli and optimization of the medium components for HA production. Methodology and results: A fragment of an approximate size of 1.5 kb that encodes the hyaluronan synthase (hasA) gene from S. zooepidemicus ATCC 39920 was amplified by PCR using hasA-specific primers. The hasA gene was ligated into the bacterial expression vector pLbADH and transformed into the expression host, Escherichia coli BL21 strain. Then, genetically engineered E. coli strain BL21 was used for the production of HA by fermentation using different glucose concentration (10-50 g/L) and different IPTG concentration (0.1, 0.5 and 1.0 mM) in shake flask culture. Amongst varying glucose concentrations, results showed that 50 g/L glucose with nutrient rich media containing nitrogen source was able to produce the highest HA concentration (0.115 ± 0.002 g/L). With addition of 1.0 mM IPTG, HA production reached a peak 0.532 ± 0.026 g/L which is around fivefold higher compared to without IPTG. Conclusion, significance and impact of study: The hasA gene was cloned from S. zooepidemicus and successfully expressed in recombinant E. coli BL21 cells. This low molecular weight HA is gaining more importance in medical and cosmetic application due to possess pronounced free radical scavenging and antioxidant activities.

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Section: Effect Of Nutrient Rich Media On Ha Fermentation By Recombinsupporting

confidence: 91%

Cloning and expression of hyaluronan synthase (hasA) in recombinant Escherichia coli BL21 and its hyaluronic acid production in shake flask culture

Lai¹,

Teo²

2019

MJM

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“…The commercial competitiveness of hyaluronic acid is limited due to the high (more than 80 %) production costs of fermentation medium [42]. To improve the efficiency of fermentation processes and reduce production costs, low-cost substrates should be utilized, such as agroindustry byproducts [26,41], which include cheese whey [1], dairy industrial waste [29,34], steep liquor [2,31] and sugarcane molasses [2,32].…”

Section: Introductionmentioning

confidence: 99%

Biomedical Potential of Hyaluronic Acid from Streptococcus zooepidemicus Produced in Sugarcane Molasses

Duffeck

Pan

Saikawa

et al. 2020

BJD

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Hyaluronic acid is a polymer with many biomedical applications with high production costs if obtained by fermentation. However, the use of agroindustry byproducts can improve the commercial viability. The aims of this work were to optimize the production of hyaluronic acid by Streptococcus zooepidemicus in sugarcane molasses medium supplemented with nutrients and to analyze the antioxidant and antimicrobial properties of hyaluronic acid. Hyaluronic acid production was greater in molasses (0.710 g•L -1 ) than in glucose (0.469 g•L -1 ). Only glutamine exhibited positive effects on production (0.843 g•L -1 ) in Erlenmeyer flasks. Using a bioreactor under 100 rpm agitation, production was three times greater than in flasks (2.55 g•L -1 ) and no differences were observed at higher agitation rates of 300 rpm. Hyaluronic acid produced presented with approximately 40 % (1 g•L -1 ) of antioxidant activity but did not show antimicrobial activity. Hyaluronic acid produced in molasses could be used in formulations as an antioxidant.

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Effects of different nitrogen sources on the production of Hyaluronic acid by Streptococcus

Cited by 2 publications

References 3 publications

Cloning and expression of hyaluronan synthase (hasA) in recombinant Escherichia coli BL21 and its hyaluronic acid production in shake flask culture

Cloning and expression of hyaluronan synthase (hasA) in recombinant Escherichia coli BL21 and its hyaluronic acid production in shake flask culture

Biomedical Potential of Hyaluronic Acid from Streptococcus zooepidemicus Produced in Sugarcane Molasses

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