Effects of different nitrogen application rates on the quality and metabolomics of cigar tobacco

Yang, Rongzhou; Yang, Jun-Liang; Yu, Jae Hyeon; Wang, Sheliang; Yang, Chunlei; Xu, Fangsen

doi:10.1002/agj2.20983

Cited by 11 publications

(8 citation statements)

References 33 publications

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“…Tobacco is considered as one of the important industrial and economic crops 6 . Among the various cigarette products, cigar has attracted extensive attention owing to the characteristics of hand-made and particularity of raw material 7 . Cigar was totally made by cigar tobacco leaves (CTLs) without filter and wrapping paper 8 .…”

Section: Introductionmentioning

confidence: 99%

Study on the chemical compositions and microbial communities of cigar tobacco leaves fermented with exogenous additive

Cai

Zheng

et al. 2022

Sci Rep

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Fermentation process plays an important role in the biochemical properties and quality of cigar tobacco leaves (CTLs). In industry, exogenous additive (EA) was usually adopted for improving the quality of CTLs during fermentation. However, the mechanism of enhanced quality of CTLs fermented with EA was confused. Herein, the chemical compositions and microbial communities of CTLs during fermentation with EA were analyzed. The increased contents of total nitrogen and total sugar, as well as the improved consumption rate of reducing sugar in CTLs were found with the addition of EA. Besides, fermentation with EA reduced the content of total nonvolatile organic acid, especially unsaturated fatty acid. The contents of total and several representative aroma components were improved. Additionally, the increased abundance of Staphylococcus and decreased abundance of Aspergillus were detected. Combined with the changes of chemical compositions and microbial communities, it was confirmed that the carbohydrates and alcohols originated from EA promote the enrichment of Staphylococcus and accelerate biochemical reactions, such as Maillard reaction and esterification reaction, thus improving the contents and quality of aroma components in CTLs. This study demonstrated the mechanism of enhanced quality of CTLs fermented by EA, which provides more ideas for developing novel and efficient EAs.

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Section: Introductionmentioning

confidence: 99%

Study on the chemical compositions and microbial communities of cigar tobacco leaves fermented with exogenous additive

Cai

Zheng

et al. 2022

Sci Rep

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“…The cigar seedlings were transplanted in Cuiba (E 109°47'42 ", N 30°27'27"), Enshi prefecture, Hubei province on May 16, 2021 and the apex was removed on July 2. Fertilizer application followed the description [30]. Middle leaves were sampled for the transcriptomic analysis on the July 22, 26 and 30, respectively.…”

Section: Discussionmentioning

confidence: 99%

Genome-scale mRNA transcriptomic insights into the mature growth dynamic of Cigar tobacco leaves

Yang

Wan

Qiao

et al. 2022

Preprint

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The harvest time is a key factor for cigar leaves with high quality, which varies greatly depending on the environment. Here, we performed a genome-scale mRNA transcriptomic analysis on the cigar cultivar CX-26 (Nicotiana tabacum L.) to evaluate the relationship between gene expression and growth state. The leaves were harvested with 67 (T1), 71(T2) and 75 (T3)-day growth. A total of 80,502 genes were detected in the CX-26 leaves, of which 64,611 genes were annotated on the reference genome. Principal component analysis showed that T1 and T2 leaves had a high overlapping pattern, while T3 leaves were distinct. Indeed, T1 and T2 leaves had fewer differential expressing genes (DEGs), while T3 leaves had 26,456 DGEs from T2 leaves, supporting the distinct growth of T3 leaves. GO annotations mainly enriched the photosynthesis-related metabolic process, catalytic activity and binding biological processes. KEEG analysis identified the key pathways including photosynthesis-antenna proteins, plant hormone/MAPK signaling pathway and plant-pathogen interaction. The maturity regulation and defense response-associated hormones abscisic acid and jasmonate acid were higher in the T3 leaves than that in T1 and T2 leaves confirming the KEEG analysis. Furthermore, several photosynthesis-related enzymes and a transcription factor were highlighted in the gene regulatory network, which might regulate the dynamics of carbohydrate metabolism, lipid metabolism and energy metabolism. In summary, our study provides insight into the growth state of CX-26 cigar leaves.

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“…The cigar seedlings were transplanted in Cuiba (109°47′42″ E, 30°27′27″ N), Enshi prefecture, Hubei province on May 16, 2021, and the apex was removed on July 2. The fertilizer application followed the description (Yang et al., 2022). Middle leaves were sampled for the transcriptomic analysis on 22nd, 26th, and 30th of July, respectively.…”

Section: Methodsmentioning

confidence: 99%

Genome‐scale transcriptomic insights into the mature growth dynamic of tobacco leaves

et al. 2023

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The harvest time is a key factor for cigar leaves with high quality, which varies greatly depending on the environment. In this study, we performed a growth characterization of the cigar tobacco CX-26 (Nicotiana tabacum L.) from the cluster stage to the vigorous stage and mature stage. Further, soil plant analysis development measurement of three middle leaves with 67 (T1), 71 (T2), and 75 days (T3) of growth after transplant indicates that the maturity might change from 71 to 75 days of growth. A genome-scale transcriptome was conducted to explore the gene expression dynamics of this maturity change. A total of 80,502 genes were detected in the CX-26 leaves, of which 64,611 genes were annotated on the reference genome. T1 and T2 leaves had fewer differential expressing genes (DEGs), while T3 leaves had 26,456 DEGs from T2 leaves, supporting the distinct growth of T3 leaves. Analyses of Gene ontology, Kyoto encyclopedia of genes and genomes identified that DEGs are involved inkey pathways including photosynthesis-antenna proteins, plant hormone/mitogenactivated protein kinase signaling pathway, and plant-pathogen interaction. Abscisic acid and jasmonate acids positively regulate leaf senescence and chlorophyll degradation and were higher in the T3 leaves compared with T1 and T2 leaves. In contrast, the total sugar was reduced in T3 leaves compared with T1 and T2 leaves, indicating the overripe state of the T3 leaves. Furthermore, several photosynthesis-related enzymes and a transcription factor were highlighted in the gene regulatory network, which might regulate the dynamics of carbohydrate metabolism, lipid metabolism, and energy metabolism. In summary, our study provides insight into the growth state of CX-26 cigar leaves.Abbreviations: ABA, abscisic acid; bZIP, basic leucine zipper; DEGs, differential expression genes; DHAR1, Dehdroascorbate reductase 1; GO, gene ontology; HSV, hue saturation value; JA, jasmonate acid; KEGG, Kyoto encyclopedia of genes and genomes; LHC, chlorophyll protein complex; MAPK, mitogen-activated protein kinase; PCA, principal component analysis; PHYA, Phytochrome A; SPA, suppressor of phyA-105 protein; SPAD, soil plant analysis development.

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Effects of different nitrogen application rates on the quality and metabolomics of cigar tobacco

Cited by 11 publications

References 33 publications

Study on the chemical compositions and microbial communities of cigar tobacco leaves fermented with exogenous additive

Study on the chemical compositions and microbial communities of cigar tobacco leaves fermented with exogenous additive

Genome-scale mRNA transcriptomic insights into the mature growth dynamic of Cigar tobacco leaves

Genome‐scale transcriptomic insights into the mature growth dynamic of tobacco leaves

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