Effects of different lipid sources on growth, digestive enzyme activity, and fatty acid composition in juvenile Chinese mitten crab, 
            <i>Eriocheir sinensis</i>

Wang, Jianyi; Yang, Ziyi; Wei, Banghong; Shi, Qiuyan; Yang, Xiaozhen; Cheng, Yongxu

doi:10.3724/sp.j.1118.2017.16322

Cited by 8 publications

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“…Nutritional indices can reflect the physiological status and metabolism of crustaceans and were generally affected by dietary nutrients (Long, Wu, et al., 2017; Ma et al., 2017). For lipid metabolism, previous studies have demonstrated that appropriately higher dietary total lipid content could increase the lipase (LPS) activity in the hepatopancreas of juvenile E. sinensis (Yang et al., 2011, dietary lipid content: 10.07% dry diet), juvenile mud crab Scylla paramamosain (Xu et al., 2017, dietary lipid content: 8.14% dry diet) and juvenile red claw crayfish Cherax quadricarinatus (Lu et al., 2018, dietary lipid content: 7% dry diet), but excessive dietary total lipid content would inhibit the LPS activity. In this study, the highest activity of LPS in the hepatopancreas of female crabs was detected in the ND mode, followed by the TD mode and FD modes.…”

Section: Discussionmentioning

confidence: 99%

“…Several studies have investigated the replacement of traditional or natural diets by commercial formulated diets for juvenile E. sinensis , with a focus on survival, gonad development (Pan, Xiao, Zhang, & Luan, 2005), growth performance, digestive physiology (Yang, Yang, Zhao, Fan, & Chen, 2011; Yang et al., 2013), immune and disease resistance (Jiang et al., 2017) and biochemical composition (Que et al., 2012). However, fewer studies have investigated adult crabs, where only the effects of the different diets on growth performance, cost or nutritional quality have been investigated (Pan et al., 2016; Zhuang et al., 2016).…”

Section: Introductionmentioning

confidence: 99%

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Key metabolic and enzymatic adaptations underlie the benefits of formulated diets in the adult female Chinese mitten crabEriocheir sinensis

et al. 2020

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Natural and traditional diets have been gradually replaced with formulated diets for the Chinese mitten crab Eriocheir sinensis culture, but it is unclear what effects different feeding modes have on digestive physiology and metabolism of E. sinensis. This study investigated the effects of three feeding modes, formulated diets (FD), natural diets (ND, trash fish and freshwater snails) and traditional diets (TD, soybean, wheat, broad bean and corn) on digestive enzyme activity, physiological indices and metabolomics of adult female E. sinensis. The ND had the highest activities of trypsin and lipase in the hepatopancreas, while the highest amylase activity was detected in TD. The highest content of hepatopancreas triglyceride was detected in FD, while the highest levels of lipoprotein lipase, hepatic lipase, lactic acid, malonaldehyde, acid phosphatase and alkaline phosphatase in the hepatopancreas, as well as the total cholesterol, high‐density lipoprotein cholesterol, low‐density lipoprotein cholesterol and γ‐glutamyltransferase in serum were detected in ND. The TD had the highest content of glucose and uric acid in the hepatopancreas as well as the free cholesterol, lactic acid, aspartate aminotransferase and alanine aminotransferase in the serum. The metabolomics showed that most differential metabolites involved in amino acid anabolism in serum and hepatopancreas were significantly up‐regulated in FD. The metabolites involved in energy metabolism and gluconeogenesis in the hepatopancreas were significantly down‐regulated in FD. These results suggested that feeding with formulated diets promotes amino acid synthesis and reduces energy metabolism, which leads to more protein and glucose deposition in tissue.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Key metabolic and enzymatic adaptations underlie the benefits of formulated diets in the adult female Chinese mitten crabEriocheir sinensis

et al. 2020

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“…Lipid was the main dietary sources for mitten crab to obtain energy for the growth and development. A lot of energy was required for the molt in the life history of mitten crab, thus a relatively stabilized activity of lipase was needed for the crustaceans to obtain enough energy at the same dietary lipid level [ 48 ].…”

Section: Discussionmentioning

confidence: 99%

Effects of the complete replacement of fish oil with linseed oil on growth, fatty acid composition, and protein expression in the Chinese mitten crab (Eriocheir sinensis)

et al. 2018

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BackgroundThe finite marine resources make it difficult for us to obtain enough fish oil (FO) used in aquatic feeds. Another sustainable ingredients should be found to substitute FO. The effects of replacing FO with vegetable oil have been studied in a variety of crustaceans, but most studies have focused on the phenotypic effects. Little is known about the mechanisms of the effects.MethodsTo understand the molecular responses during the replacement of FO in Eriocheir sinensis, we investigated the effects of feeding FO or linseed oil (LO) on growth performance, digestive enzyme activity, fatty acid composition and protein expression in E. sinensis. Twenty-four juvenile crabs were fed diets containing FO or LO for 112 days. Weight, carapace length and width were recorded. Fatty acid composition of the diets and the hepatopancreas and protein expression in the hepatopancreas were analyzed.ResultsGrowth performance and molting interval were unchanged by diet. Crabs fed FO and LO had same activity of lipase and amylase, but comparing with crabs fed LO, crabs fed FO had higher trypsin activity and lower pepsin activity. Hepatopancreas fatty acid composition changed to reflect the fatty acid composition of the diets. In total, 194 proteins were differentially expressed in the hepatopancreas between the diets. Expression of heat shock proteins was higher in crabs fed LO. Expression of fatty acid synthase, long-chain fatty acid transport protein 4, acyl-CoA delta-9 desaturase, and fatty acid-binding protein 1, was higher in crabs fed FO.ConclusionsThe substitution of FO with LO didn’t have any effects on the growth and molting of mitten crab, but could significantly decrease the ability of mitten crab to cope with stress. The high content of HUFAs in the hepatopancreas of mitten crab fed FO is due to the high abundance of the proteins relative to the transport of the HUFAs. These findings provide a reason of the high content of EPA and DHA in crabs fed with FO, and provide new information for the replacement of FO in diets of mitten crab.Electronic supplementary materialThe online version of this article (10.1186/s12953-018-0135-7) contains supplementary material, which is available to authorized users.

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“…For omnivorous species, amylase is important for digestion. A previous study showed that mitten crabs fed with a linseed oil diet (2.03) had a lower feed coefficient than mitten crabs fed with a soybean oil diet (2.46) ( Wang et al, 2017 ). We speculate that improvement of the feed coefficient in the linseed oil group is mainly due to the higher abundance of amylase, as well as trypsin, attributable to LNA.…”

Section: Discussionmentioning

confidence: 99%

Proteomic Analysis of the Hepatopancreas of Chinese Mitten Crabs (Eriocheir sinensis) Fed With a Linoleic Acid or α-Linolenic Acid Diet

et al. 2018

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As representatives of n-6 and n-3 fatty acids, many studies have analyzed the use of soybean oil and linseed oil rich in linoleic acid (18:2n-6, LA) and α-linolenic acid (18:3n-3, LNA) as better substitutes for fish oil. In aquatic animals, different dietary ratios of LA and LNA could have significant effects on growth, lipid metabolism, immune response, and reproduction. To assess the nutritive value of these two fatty acids in Chinese mitten crab (Eriocheir sinensis), we performed transcriptome analysis and label-free quantification proteomic analysis of the hepatopancreas from mitten crabs fed with LA or LNA diet. Parallel reaction monitoring was used to confirm the reliability of the proteomic analysis. A total of 186 proteins were differentially expressed with fold change ≥1.5 or ≤0.666. Among the 186 proteins, 116 were upregulated and 70 were downregulated in the LA than LNA. Most of these proteins participate in cellular process and metabolism process and have molecular functions such as binding and catalytic activity; the cellular component of these proteins are cell, cell part, membrane, and membrane part. A total of 18 proteins were identified to be related to lipid, carbohydrate, and protein metabolism, and they mainly participate in digestive enzyme activities, fatty acid transport, and glycolysis. Our results provide new insights for further investigation into the replacement of fish oil from mitten crabs with vegetable oils and enable us to better understand the different roles and nutrition value of LA and LNA in mitten crabs.

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Effects of different lipid sources on growth, digestive enzyme activity, and fatty acid composition in juvenile Chinese mitten crab, Eriocheir sinensis

Cited by 8 publications

References 0 publications

Key metabolic and enzymatic adaptations underlie the benefits of formulated diets in the adult female Chinese mitten crabEriocheir sinensis

Key metabolic and enzymatic adaptations underlie the benefits of formulated diets in the adult female Chinese mitten crabEriocheir sinensis

Effects of the complete replacement of fish oil with linseed oil on growth, fatty acid composition, and protein expression in the Chinese mitten crab (Eriocheir sinensis)

Proteomic Analysis of the Hepatopancreas of Chinese Mitten Crabs (Eriocheir sinensis) Fed With a Linoleic Acid or α-Linolenic Acid Diet

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