Effects of different levels of dietary Citrus Limon essential oil on some blood parameters and antioxidant status in Afshari Ewes

Abstract

Citrus Limon Oil (CLO) is known as antioxidant resource and contains limonoids, flavonoids and phenolic compounds. This study was conducted to investigate the effects of different levels of CLO on blood parameters and antioxidant status in Afshari ewes. Six adults Afshari ewes (3-4 years old and 51±5 kg) were randomly allocated to 3×3 Latin square design with three diets in 21 days period. Dietary treatments included:1) control diet, 2) control diet with 200 mg/day CLO, and 3) control diet with 400 mg/day CLO. To evaluate the antioxidant effect of the CLO, sustainable elimination of free radicals by DPPH and ABTS methods were used. The antioxidant activity of essential oils in DPPH method at doses of 32.5, 45, 130, 260 and 520 mg/ml were 9, 16, 31, 49 and 89%, respectively. Also, antioxidant activity of essential oils in ABTS method at doses of 32.5, 45, 130, 260 and 520 mg/ml were 49, 73, 81, 89 and 95%, respectively. CLO treatments did not affect glucose, blood urea nitrogen, albumin, total protein, low density lipoprotein, while improved the concentration of high-density lipoprotein (P>0.01). Results showed that supplementation with CLO significantly decreased (P<0.01) cholesterol, triglycerides and very low density lipoprotein concentrations compared with control. There was no significant difference in analyzed blood bio-chemicals and serum enzymes level between different antioxidant activity methods and groups, suggesting general well-being of ewes. These results suggest that, CLO supplementation had a positive impact on blood traits and antioxidant status of the Afshari ewes.

Cholesterol‐lowering activity of natural mono‐ and sesquiterpenoid compounds in essential oils: A review and investigation of mechanisms using in silico protein–ligand docking

“…In afshari ewes, treatment with 200 mg/day of Citrus limon EO resulted in a decrease in cholesterol, TGs, and LDL by 15%, 19%, and 5%, respectively, while increasing HDL by 16%. Treatment with 400 mg/day showed even greater changes, with an increase in HDL by 23%, and a decrease in TC, TGs, and VLDL by 27%, 32%, and 41% respectively (Mojtahedin et al, 2018). In streptozotocin‐induced diabetic rats treated with D‐limonene at 50 mg/kg/day for 28 days, results showed a decreased in LDL by 20%, a decrease in TC by 57%, and a decrease in TGs by 20% (Bacanlı et al, 2017).…”

Development of Avocado and Lemon Oil Emulgels Based on Natural Products: Phycocyanin and Pectin

“…Among essential oils, lemon essential oil has properties of great interest for its applications in fields such as cosmetics, food, and medicine. It has beneficial health effects by lowering cholesterol and triglycerides without affecting other blood biochemical parameters [ 12 ].…”

Effect of Chinese Herbs on Serum Biochemical Parameters, Immunity Indices, Antioxidant Capacity and Metabolomics in Early Weaned Yak Calves

“…Consumption of A. sinensis polysaccharides decreased the serum concentration of total cholesterol in diabetic mice and accelerated serum FFA oxidation in mice [ 19 , 20 ]. In addition, botanical essential oils, a main component of A. sinensis , lowered the serum concentration of total cholesterol in pigs and sheep [ 21 , 22 ]. In the current study, serum TG concentrations were generally lower in yak calves consuming the herbal extracts, especially G. uralensis , than in control yak calves.…”