Effects of different land use patterns on nifH genetic diversity of soil nitrogen-fixing microbial communities in Leymus Chinensis steppe

Zou, Yukun; Zhang, Jingni; Dian-lin, Yang; Chen, Xiurong; Zhao, Junjie; Xiu, Weiming; Lai, Xin; Li, Gang

doi:10.1016/j.chnaes.2011.03.004

Cited by 22 publications

(16 citation statements)

References 30 publications

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“…Although, Hsu and Buckley , in their long‐term tillage experiment showed the functional significance of changes in the diazotrophic community structure as N‐fixation rates varied as a function of diazotroph community diversity. Nitrogen‐fixing microbial communities in Leymus chinensis steppe were significantly ( p < 0.05) influenced by the levels of nitrate nitrogen, total phosphorus, available phosphorus contents, and pH value . The lowest number of N 2 ‐fixing bacteria was observed in the rhizosphere soil with high N fertilization .…”

Section: Discussionmentioning

confidence: 95%

Dynamics of soil diazotrophic community structure, diversity, and functioning during the cropping period of cotton (Gossypium hirsutum)

Rai

Singh

Annapurna

2014

J. Basic Microbiol.

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The soil sampled at different growth stages along the cropping period of cotton were analyzed using various molecular tools: restriction fragment length polymorphism (RFLP), terminal restriction length polymorphism (T-RFLP), and cloning-sequencing. The cluster analysis of the diazotrophic community structure of early sampled soil (0, 15, and 30 days) was found to be more closely related to each other than the later sampled one. Phylogenetic and diversity analysis of sequences obtained from the first (0 Day; C0) and last soil sample (180 day; C180) confirmed the data. The phylogenetic analysis revealed that C0 was having more unique sequences than C180 (presence of γ-Proteobacteria exclusively in C0). A relatively higher richness of diazotrophic community sequences was observed in C0 (S(ACE) : 30.76; S(Chao1) : 20.94) than C180 (S(ACE) : 18.00; S(Chao1) : 18.00) while the evenness component of Shannon diversity index increased from C0 (0.97) to C180 (1.15). The impact of routine agricultural activities was more evident based on diazotrophic activity (measured by acetylene reduction assay) than its structure and diversity. The nitrogenase activity of C0 (1264.85 ± 35.7 ηmol of ethylene production g(-1) dry soil h(-1) ) was statistically higher when compared to all other values (p < 0.05). There was no correlation found between diazotrophic community structure/diversity and N2 fixation rates. Thus, considerable functional redundancy of nifH was concluded to be existing at the experimental site.

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Section: Discussionmentioning

confidence: 95%

Dynamics of soil diazotrophic community structure, diversity, and functioning during the cropping period of cotton (Gossypium hirsutum)

Rai

Singh

Annapurna

2014

J. Basic Microbiol.

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“…Nitrogenase is the enzyme responsible for nitrogen fixation while nifH is the gene that encodes for the iron protein subunit of nitrogenase and is highly conserved among all nitrogen-fixing groups, making it an ideal molecular marker for these microorganisms (Deslippe and Egger, 2006). Cloning and sequencing of nifH gene have provided a large, rapidly expanding database of nifH sequences from diverse environments (Zehr et al, 2003), such as polar and cold soils (Olson et al, 1998;Deslippe and Egger, 2006;Zhang et al, 2006), aquatic habitats (Steward et al, 2004;Moisander et al, 2008;Hamilton et al, 2011), agricultural soils (Coelho et al, 2009;Zou et al, 2011). However, the diversity of nitrogen-fixing bacteria was still poorly described and many microorganisms remain to be discovered (as reviewed in Gaby and Buckley, 2011).…”

Section: Introductionmentioning

confidence: 99%

High diversity of nitrogen-fixing bacteria in upper reaches of Heihe River, Northwestern China

Tai¹,

Mao²,

Liu³

et al. 2013

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Vegetation plays a key role to water conservation in southern Qilian Mountains (Northwestern China), the upper reaches of Heihe River. Nitrogen-fixing bacteria are crucial for vegetation protection because they can supply plants with nitrogen source. Nevertheless, little is known about nitrogen-fixing bacteria in this region. In present study, nifH gene clone libraries were established for detecting the difference of nitrogen-fixing bacterial communities between Potentilla parvifolia shrub and Carex alrofusca meadow in the southern Qilian Mountains. All the identified nitrogen-fixing bacterial clones belonged to Proteobacteria. At the genus level, the Azospirillum sp. was only detected in shrub soil while Thiocapsa sp., Derxiasp., Ectothiorhodospira sp., Mesorhizobium sp., Klebsiella sp., Ensifer sp., Methylocella sp. and Peseudomonas sp. were just detected in meadow soil. Shannon–Wiener index of nifH gene ranged from 1.5 to 2.8 and was higher in meadow soil than shrub soil. Contrarily, the nifH gene copies and CFUs of cultured nitrogen-fixing bacteria ranged from 0.4 × 10⁷ to 6.9 × 10⁷ copies g⁻¹ soil and 0.97 × 10⁶ to 12.78 × 10⁶ g⁻¹ soil, respectively. Furthermore, both of them were lower in meadow soil than shrub soil. Statistical analysis revealed that diversity and copies of nifH gene mostly correlated with aboveground biomass in shrub soil. In meadow soil, nifH gene diversity was principally affected by altitude while copies did by soil available K

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“…Plant-specific differences in the nitrogen-fixing bacterial community may be related to plant species, biomass, the chemical composition of litter and root exudates (Shaffer et al, 2000;Bürgmann et al, 2005;Hsu and Buckley, 2009;Knelman et al, 2012). Soil physicochemical factors, such as pH and water content (Zhan and Sun, 2011), microbial biomass carbon and microbial biomass nitrogen (Hayden et al, 2010), total nitrogen and total potassium (Teng et al, 2009;Hayden et al, 2010), total phosphorus and reactive phosphorus (Reed et al, 2010;Zou et al, 2011;Romero et al, 2012), electrical conductivity (Hayden et al, 2010;Hamilton et al, 2011) and nutrient level (Jasrotia and Ogram, 2008;Zhan and Sun, 2012), have also been identified as drivers of nifH gene diversity and abundance in many environments.…”

Section: S Tai Et Al: High Diversity Of Nitrogen-fixing Bacteriamentioning

confidence: 99%

“…Nitrogenase is the enzyme responsible for nitrogen fixation, and nifH is the gene that encodes for the iron protein subunit of nitrogenase, which is highly conserved among all nitrogen-fixing groups and serves as an ideal molecular marker for these microorganisms (Deslippe and Egger, 2006). The cloning and sequencing of the nifH gene have provided a large and rapidly expanding database of nifH sequences from a number of diverse environments (Zehr et al, 2003), including cold polar soils (Olson et al, 1998;Deslippe and Egger, 2006;Zhang et al, 2006), aquatic habitats (Steward et al, 2004;Moisander et al, 2008;Hamilton et al, 2011) and agricultural soils (Coelho et al, 2009;Zou et al, 2011). However, the diversity of nitrogen-fixing Published by Copernicus Publications on behalf of the European Geosciences Union.…”

Section: Introductionmentioning

confidence: 99%

High diversity of nitrogen-fixing bacteria in the upper reaches of the Heihe River, northwestern China

et al. 2013

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Vegetation plays a key role in water conservation in the southern Qilian Mountains (northwestern China), located in the upper reaches of the Heihe River. Nitrogen-fixing bacteria are crucial for the protection of the nitrogen supply for vegetation in the region. In the present study, nifH gene clone libraries were established to determine differences between the nitrogen-fixing bacterial communities of the Potentilla parvifolia shrubland and the Carex alrofusca meadow in the southern Qilian Mountains. All of the identified nitrogen-fixing bacterial clones belonged to the Proteobacteria. At the genus level, Azospirillum was only detected in the shrubland soil, while Thiocapsa, Derxia, Ectothiorhodospira, Mesorhizobium, Klebsiella, Ensifer, Methylocella and Pseudomonas were only detected in the meadow soil. The phylogenetic tree was divided into five lineages: lineages I, II and III mainly contained nifH sequences obtained from the meadow soils, while lineage IV was mainly composed of nifH sequences obtained from the shrubland soils. The Shannon–Wiener index of the nifH genes ranged from 1.5 to 2.8 and was higher in the meadow soils than in the shrubland soils. Based on these analyses of diversity and phylogeny, the plant species were hypothesised to influence N cycling by enhancing the fitness of certain nitrogen-fixing taxa. The number of nifH gene copies and colony-forming units (CFUs) of the cultured nitrogen-fixing bacteria were lower in the meadow soils than in the shrubland soils, ranging from 0.4 × 10⁷ to 6.9 × 10⁷ copies g⁻¹ soil and 0.97 × 10⁶ to 12.78 × 10⁶ g⁻¹ soil, respectively. Redundancy analysis (RDA) revealed that the diversity and number of the nifH gene copies were primarily correlated with aboveground biomass in the shrubland soil. In the meadow soil, nifH gene diversity was most affected by altitude, while copy number was most impacted by soil-available K. These results suggest that the nitrogen-fixing bacterial communities beneath Potentilla were different from those beneath Carex

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Effects of different land use patterns on nifH genetic diversity of soil nitrogen-fixing microbial communities in Leymus Chinensis steppe

Cited by 22 publications

References 30 publications

Dynamics of soil diazotrophic community structure, diversity, and functioning during the cropping period of cotton (Gossypium hirsutum)

Dynamics of soil diazotrophic community structure, diversity, and functioning during the cropping period of cotton (Gossypium hirsutum)

High diversity of nitrogen-fixing bacteria in upper reaches of Heihe River, Northwestern China

High diversity of nitrogen-fixing bacteria in the upper reaches of the Heihe River, northwestern China

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