Effects of different intensities of exercise on folliculogenesis in mice: Which is better?

Abstract

Objective: Exercise is a risk factor for infertility in women. However, research on the effects of different intensities of exercise on folliculogenesis has not yielded clear results. This study was conducted to analyze the effects of differences in the intensity of exercise on folliculogenesis in mice. Methods: Nineteen female BALB/c mice (age, 3–4 months; weight, 13–25 g) were randomly divided into four groups: control, mild exercise, moderate exercise, and high-intensity exercise. The mice in the exercise groups engaged in swimming, with additional loads of 3%, 6%, or 9% of body weight, respectively. There were five swimming sessions per week for 4 weeks, with a gradually increasing duration every week. At the end of the treatment, ovarian extraction was carried out and hematoxylin and eosin staining was performed to identify folliculogenesis. Results: There were significant differences in the number of total follicles between the control and moderate-exercise groups (p=0.036) and between the mild- and moderate-exercise groups (p=0.005). The mean number of primary follicles was higher in the moderate-exercise group than in the mild-exercise group (p=0.006). The mean number of secondary, tertiary, and Graafian follicles did not differ significantly among groups (p≥0.05). However, the number of total follicles and follicles in each phase tended to increase after exercise, especially moderate-intensity exercise. Conclusion: Exercise of different intensities affected the total number of follicles and primary follicles. The number of follicles of each phase tended to increase after exercise. Moderate-intensity exercise had better effects than other intensities of exercise.

The Combination of High Calorie Diet and Moderate Interval Training Decreases Follicle Stimulating Hormone (FSH) Level In Rats

“…Regular exercise is an important component of supporting long-term weight loss. Changes in body weight occur as a result of compensatory mechanisms by the body [22]. Different results were shown with this study, a study on 162 women between the ages of 19 and 42 and found that moderate-intensity physical activity was associated with higher level of anti-Müllerian hormones and FSH.…”

Continuous Swimming Training in Moderate Intensity Decreased Follicle Stimulating Hormone Level in Female Mice on a High-Calorie Diet

“…Changes in physical activity have a positive effect on sexual health in the form of: more frequent sexual intercourse, better vaginal lubrication, and overall sexual function 5 years later (Wekker et al, 2018). Exercise with intensity moderate indicates a tendency for a higher number of follicles (Rahayu et al, 2021). The study also shows that moderate-intensity exercise has a positive effect on ovarian reserve due to the number of apoptotic follicles in the group undergoing less sports training.…”

The Effect of Exercise Intensity on Anthropometric Parameters and Renal Damage in High Fructose- Induced Mice

“…Differences in intensity between exercise groups using weights placed on the mice's tails. Swimming exercise was carried out with a load of 3% of body weight for low-intensity exercise (Rahayu et al, 2021), 6% of body weight for moderateintensity exercise (Antoni et al, 2022), and 9% of body weight for high-intensity exercise (Rahayu et al, 2021). The water temperature was maintained between 30-32°C (Evangelista et al, 2003).…”