Effects of Different Growth Media on In Vitro Seedling Development of an Endangered Orchid Species Sedirea japonica

An, Jiae; Kim, Pyoung Beom; Park, Hyeong Bin; Kim, Seong-Jun; Park, Hwan Joon; Lee, Chang Woo; Lee, Byoung-Doo; Kim, Nam Young; Hwang, Jin-Woo

doi:10.3390/plants10061193

Cited by 9 publications

(4 citation statements)

References 45 publications

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“…On the other hand, the use of Half-MS strength medium also resulted in the best growth and development of PLBs in Dendrobium var. Sonia [6], Cattleya bicolor orchid [7], Sedirea japonica orchid [13], and Dendrobium seedlings [10]. The use of Half-MS strength medium also proves that for the growth and development of PLBs, several types of orchids do not require high concentrations.…”

Section: Resultsmentioning

confidence: 99%

“…Enrichment with 0.6 gL -1 coconut water, 30 gL -1 banana homogenate, and 10 gL -1 apple-homogenate into MS medium very effectively stimulated the regeneration of orchid PLBs Sedirea japonica and increasing plantlet fresh weight. The results of this study have been standardized as a medium for mass production and conservation of this rare epiphytic orchid [13,14]. The use medium NP + 100 gL -1 banana in in vitro culture media increases the growth rate of D. lineale orchids [15].…”

Section: Introductionmentioning

confidence: 98%

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Protocorm Like Bodies (PLBs) dendrobium orchid “Gatton Sunray”

et al. 2023

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The Dendrobium Orchid Gatton Sunray is a hybrid orchid of Dendrobium pulchellum × Dendrobium illustre. A study was aimed to find the best type of medium and type of banana for the multiplication of Protocorm-Like Bodies (PLBs) of this orchid. This research was conducted from February to September 2020 at the Plant Tissue Culture Laboratory, Faculty of Agriculture, The University of Bengkulu. Treatments included type of media and cultivar of ripe banana fruit, added as source of organic complex, consisted of 4 cultivars, namely Ambon Curup, Kepok, Tanduk and Mas. Ripe bananas were mashed and added to the in vitro medium as much as 50 g.L-1. Types of media consist of Murashige and Skoog (MS), ½ Doses of Murashige and Skoog (MS) media, and Complex of fertilizer media. The results showed that the ½ MS medium added with 50 g.L-1 cv. Kepok fruit produced the greatest multiplication (11.8 PLBs per jar), the best growth of PLBs, including the greatest PLBs number (28.5 PLBs per jar), the heaviest fresh weight (1.30 g per jar) and the longest PLBs (2.56 cm).

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 98%

Protocorm Like Bodies (PLBs) dendrobium orchid “Gatton Sunray”

et al. 2023

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“…The promoting effect of the Hyponex medium on the GGB proliferation and differentiation might be correlated to the relatively high content of trace elements and microelements in the medium. Despite the wide application of Hyponex in the tissue culture of orchids (An et al, 2021;Chen et al, 2015;Ket et al, 2004), there have been no reports on the use of the Hyponex medium in the tissue culture of ferns.…”

Section: Paraffin Sections Of An Embryo and Ggb Of Adiantum Reniforme...mentioning

confidence: 99%

Green Globular Body Induction and Plantlet Regeneration of Endangered Fern Adiantum reniforme var. sinense

Lin,

Shih,

Chang

et al. 2024

IJARe

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Background: Adiantum reniforme var. sinense is a pteridophyte with considerable ornamental importance and medicinal value. However, the wild population of A. reniforme var. sinense has gradually become scarce due to sparse distribution and human activities. Green Globular Bodies (GGBs) are specific tissue which use to propagate ferns in vitro. The study uses GGBs induced from sporophytic embryos to preserve and to proliferate this pteridophyte. Methods: The present study was carried out during year 2018-2020 at National Ilan University. The developing sporophyte of Adiantum reniforme var. sinense were used to induce GGBs. The optimal medium for GGBs proliferation and differentiation in terms of basal salts, plant growth regulators, sugar concentrations and gelling agents was examined. The structure of GGBs and the embryos were analyzed by paraffin section as well. Result: The result indicates the 100% GGBs induction rate from sporophytic embryos for the first time could be obtained under BA (0.1 mg L-1) and NAA (0.15 mg L-1). For subsequent proliferation of GGBs required a higher concentration of NAA (10 mg L-1). Cytokinins such BA, KT and TDZ, inhibited GGBs proliferation. In vitro optimal amount of basal salts, plant growth regulators, sugar concentrations and gelling agents for GGB proliferation were obtained as 2 g L-1 Hyponex, 10 mg L-1 NAA, 15 g L-1 sucrose and 4 g L-1 gelrite, respectively. Paraffin sections revealed histological characteristics of the sporophytic embryo and GGB.

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“…As demonstrated in previous studies, incorporating organic supplements into culture media can enhance the growth and development of various plant tissues in vitro. These supplements include apple juice, banana homogenates, coconut water, corn extracts, and potato homogenates [34,35]. These organic additives are integrated into the culture medium not only as a natural source of carbon, but also for their rich content in vitamins, fiber, hormones, and proteins [36].…”

Section: Effects Of Organic Supplements On Seedling Growthmentioning

confidence: 99%

Asymbiotic Seed Germination and In Vitro Seedling Development of the Endangered Orchid Species Cypripedium guttatum

Park,

An,

Bae

et al. 2023

Plants

Self Cite

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Cypripedium guttatum is a highly restricted terrestrial orchid that faces increasing endangerment owing to its habitat destruction and illegal collection. Compared to epiphytic orchids, terrestrial orchids such as C. guttatum have harder seed coats and more demanding in vitro germination conditions. This study aimed to develop an effective in vitro propagation system for C. guttatum to aid in its conservation. Seeds from mature capsules were subjected to various conditions, including sterilization using 1% sodium hypochlorite (NaOCl) and different light conditions, culture media, hormones, and organic supplements, to assess germination and early seedling development in vitro. Sterilization with 1% NaOCl significantly improved the germination rate, especially under dark conditions. Germination initiation occurred at 2 and 3 months in orchid seed sowing medium (OSM) and Murashige and Skoog (MS) medium, respectively. The addition of 1 mg/L naphthaleneacetic acid (NAA) further enhanced germination. However, the inclusion of organic supplements, such as apple and banana homogenates, in the culture medium led to substantial growth inhibition after 12 months. Notably, orchid maintenance medium (OMM) without organic additives proved to be the most suitable for seedling growth. The results of this study show that sterilization, appropriate light, and optimal NAA concentrations are beneficial for seed germination.

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Effects of Different Growth Media on In Vitro Seedling Development of an Endangered Orchid Species Sedirea japonica

Cited by 9 publications

References 45 publications

Protocorm Like Bodies (PLBs) dendrobium orchid “Gatton Sunray”

Protocorm Like Bodies (PLBs) dendrobium orchid “Gatton Sunray”

Green Globular Body Induction and Plantlet Regeneration of Endangered Fern Adiantum reniforme var. sinense

Asymbiotic Seed Germination and In Vitro Seedling Development of the Endangered Orchid Species Cypripedium guttatum

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