Effects of different extraction on the antibacterial and antioxidant activities of phenolic compounds of areca nut (husks and seeds)

Fan, Xiaoyuan; Jiang, Chengyu; Dai, Wenna; Jing, Huijuan; Du, Xiaojing; Peng, Mengchen; Zhang, Yangyang; Mo, Ling; Wang, Li; Chen, Xiaohua; Lou, Zaixiang; Wang, Hongxin

doi:10.1007/s11694-021-01244-7

Cited by 8 publications

(5 citation statements)

References 55 publications

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“…GC-MS data also showed that the content of phenolic compounds in EO-70 was the highest, and the antibacterial activity of EO-70 was also the strongest. We speculate that phenolic compounds were the active antibacterial components of the ANEOs, which is consistent with reports that phenols generally have strong antibacterial activity ( Fan et al., 2022 ), and the antibacterial components of areca nut are mainly polyphenol ( Jam et al., 2021 ; Raju et al., 2021 ). Overall, the active components of ANEO were preliminarily separated and enriched through negative pressure hydro-distillation compared to the traditional hydro-distillation extraction.…”

Section: Discussionsupporting

confidence: 91%

Antibacterial mechanism of areca nut essential oils against Streptococcus mutans by targeting the biofilm and the cell membrane

Liu,

Zhang,

et al. 2023

Front. Cell. Infect. Microbiol.

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IntroductionDental caries is one of the most common and costly biofilm-dependent oral diseases in the world. Streptococcus mutans is the major cariogenic pathogen of dental caries. S. mutans synthesizes extracellular polysaccharides by autologous glucosyltransferases, which then promotes bacterial adhesion and cariogenic biofilm formation. The S. mutans biofilm is the principal target for caries treatment. This study was designed to explore the antibacterial activity and mechanisms of areca nut essential oil (ANEO) against S. mutans.MethodsThe ANEOs were separated by negative pressure hydro-distillation. The Kirby-Bauer method and broth microdilution method were carried out to evaluate the antibacterial activity of different ANEOs. The antibacterial mechanism was revealed by crystal violet staining, XTT reduction, microbial adhesion to hydrocarbon test, extracellular polysaccharide production assay, glucosyltransferase activity assay, lactate dehydrogenase leaking, propidium iodide staining and scanning electron microscopy (SEM). The cytotoxicity of ANEOs was determine by MTT assay.ResultsThe ANEOs separated at different temperatures exhibited different levels of antibacterial activity against S. mutans, and the ANEO separated at 70°C showed the most prominent bacteriostatic activity. Anti-biofilm experiments showed that the ANEOs attenuated the adhesion ability of S. mutans by decreasing the surface hydrophobicity of the bacteria, prevented S. mutans biofilm formation by inhibiting glucosyltransferase activity, reducing extracellular polysaccharide synthesis, and reducing the total biofilm biomass and activity. SEM further demonstrated the destructive effects of the ANEOs on the S. mutans biofilm. Cell membrane-related experiments indicated that the ANEOs destroyed the integrity of the cell membrane, resulting in the leakage of lactic dehydrogenase and nucleic acids. SEM imaging of S. mutans cell showed the disruption of the cellular morphology by the ANEOs. The cytotoxicity assay suggested that ANEO was non-toxic towards normal oral epithelial cells.DiscussionThis study displayed that ANEOs exerted antibacterial activity against S. mutans primarily by affecting the biofilm and disrupting the integrity of the cell membrane. ANEOs has the potential to be developed as an antibacterial agent for preventing dental caries. Additionally, a new method for the separation of essential oil components is presented.

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Section: Discussionsupporting

confidence: 91%

Antibacterial mechanism of areca nut essential oils against Streptococcus mutans by targeting the biofilm and the cell membrane

Liu,

Zhang,

et al. 2023

Front. Cell. Infect. Microbiol.

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“…However, the yield tended to decrease with the elevation of power as the extraction temperature was over 75℃. Excessive power led to the degradation of phenolic compounds [3] , thereby reducing the yield of ACP. Except for the above two factors, the interaction between the other two factors is not remarkable ( p > 0.05).…”

Section: Resultsmentioning

confidence: 99%

“…As another functional component of Areca nut, phenolic compounds are rarely studied and reported at present. The phenolic compounds have the highest content in Areca nut seeds, accounting for about 30% [3] . They have antibacterial, antioxidant, hypoglycemic, antithrombotic and other biological activities [4] .…”

Section: Introductionmentioning

confidence: 99%

Optimization of ultrasonic-assisted extraction of polyphenol from Areca nut (Areca catechu L.) seeds using response surface methodology and its effects on osteogenic activity

Sun

Lü

et al. 2023

Ultrasonics Sonochemistry

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“…In a study conducted by Fan et al [ 108 ], the impact of various extraction methods on the antibacterial and antioxidant properties of phenolic compounds found in AN was assessed. The results indicated that the MIC (minimum inhibitory concentration) values for Streptococcus mutans , Staphylococcus aureus , Escherichia coli , and Pseudomonas aeruginosa were 2.50, 5.00, 15.00, and 25.00 mg/mL, respectively.…”

Section: Biomedical Applicationsmentioning

confidence: 99%

Biological Effects and Biomedical Applications of Areca Nut and Its Extract

Tong,

Xu,

Tan

et al. 2024

Pharmaceuticals

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The dried, mature fruit of the palm tree species Areca catechu L. is known as the areca nut (AN) or betel nut. It is widely cultivated in the tropical regions. In many nations, AN is utilized for traditional herbal treatments or social activities. AN has historically been used to address various health issues, such as diarrhea, arthritis, dyspepsia, malaria, and so on. In this review, we have conducted a comprehensive summary of the biological effects and biomedical applications of AN and its extracts. Initially, we provided an overview of the constituents in AN extract. Subsequently, we summarized the biological effects of AN and its extracts on the digestive system, nervous system, and circulatory system. And we elucidated the contributions of AN and its extracts in antidepressant, anti-inflammatory, antioxidant, and antibacterial applications. Finally, we have discussed the challenges and future perspectives regarding the utilization of AN and its extracts as emerging pharmaceuticals or valuable adjuncts within the pharmaceutical field.

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Effects of different extraction on the antibacterial and antioxidant activities of phenolic compounds of areca nut (husks and seeds)

Cited by 8 publications

References 55 publications

Antibacterial mechanism of areca nut essential oils against Streptococcus mutans by targeting the biofilm and the cell membrane

Antibacterial mechanism of areca nut essential oils against Streptococcus mutans by targeting the biofilm and the cell membrane

Optimization of ultrasonic-assisted extraction of polyphenol from Areca nut (Areca catechu L.) seeds using response surface methodology and its effects on osteogenic activity

Biological Effects and Biomedical Applications of Areca Nut and Its Extract

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