Effects of different exposures of hyperbaric oxygen on ligament healing in rats

Abstract

Hyperbaric oxygen (HBO) is a method of augmenting, intermittently, oxygen availability to tissues. We examined the effect of three different HBO exposures on the healing of experimentally induced ligament lacerations in the right hind limb of 44 male Wistar rats. Animals were divided into four groups after ligament injury: (a) control group, animals breathed room air a t 1 ATA (atmosphere absolute) in a hyperbaric chamber for 60 min; (b) HBO treatment at 1.5 ATA for 30 rnin once a day, (c) HBO treatment at 2 ATA for 30 min once a day, (d) 2 ATA for 60 rnin once a day. At 14 days post-ligament injury, we compared the ligaments of the four treatment groups for gross appearance, histology and expression of pro-a( I) mRNA by northern hybridization. Our results indicate that HBO was effective in promoting ligament healing compared to control (p < 0.01). Of these three exposures, HBO at 2 ATA for 60 min was the most effective, resulting in enhanced extra-cellular matrix deposition as measured by collagen synthesis.

Smoking delays chondrogenesis in a mouse model of closed tibial fracture healing

“…28 Prolonged hypoxia could be a major factor in the impaired production of cartilaginous callus in smokers, since oxygen is essential for physiologic hydroxylation of proline and lysine, a critical step in type II collagen synthesis. 29 Jorgensen et al demonstrated decreased hydroxyproline concentrations at a site of wound healing in smokers compared to nonsmokers, indicating decreased collagen synthesis in the smoking group. 30 Decreased or delayed type II collagen synthesis as a result of smoking and tissue hypoxia could directly lead to the deleterious effects of smoking on fracture healing.…”

The Effects of Hyperbaric Oxygen Therapy on Reduction of Edema and Pain in Athletes With Ankle Sprain in the Acute Phase: A Pilot Study

“…The effects of HBO 2 therapy on soft tissue injuries during sports activities, including sprains, 2 ligament injuries, [3][4][5] contusions, and muscle strains, [6][7][8][9][10] have been widely reported.…”

Effect of Combination Microcurrent Electrical Neuromuscular Stimulation and Hyperbaric Oxygen Therapy on the Regeneration of Injured Skeletal Muscle in Mice

“…Further, there has been some thought that MENS combined with icing might be a useful therapy for sports-related skeletal muscle injuries 3) . Hyperbaric oxygen (HBO) therapy is known to facilitate repair of damaged tissue [13][14][15][16][17][18][19][20][21][22][23][24][25][26][27][28] . With HBO, pure oxygen is administered in a high pressure environment of 2.0 or more atmospheres absolute (ATA) for 60 minutes.…”

“…In general, HBO is considered to be applicable to emergency conditions (carbon monoxide poisoning, severe infection, compartment syndrome, for example) and to non-emergency conditions (refractory ulcer, skin graft, chronic refractory osteomyelitis, for example) 33)34) . There have been numerous reports indicating that HBO facilitates the regeneration of soft tissue [13][14][15][16][17][18][19][20][21][22][23][24][25][26][27][28] , and this includes the regeneration required in cases of skeletal muscle injury [19][20][21][22][23][24] . The beneficial effects of HBO have been reported in relation to various specific sports injuries 21)22) [25][26][27][28] .…”