Effects of Different Drying Methods on the Antioxidant Activities of Leaves and Berries of Cayratia trifolia

Rabeta, M. S.; Lin, S. P.

doi:10.17576/jsm-2015-4402-16

Cited by 34 publications

(25 citation statements)

References 18 publications

(24 reference statements)

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“…In general, the content of flavonoids content in ovendried samples was higher than that in the freeze-dried samples. These results are not in concordance with those obtained by Rabeta and Lin (2015) [42] who studied the same drying methods on antioxidant activities (including flavonoids) of leaves and berries of Cayratia trifolia.…”

Section: Resultscontrasting

confidence: 90%

Effect of Freeze-drying and Oven-drying Methods on Flavonoids Content in two Romanian Grape Varieties

Oprică¹,

Antohe²,

Verdes³

et al. 2019

Rev. Chim.

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The aims of this work was to investigate the effect of thermal drying method (vacuum oven drying), and nonthermal drying method (freeze drying) on the flavonoids content in two red grape varieties (Cabernet Sauvignon and Merlot) from different grape components (seed, skin, and pulp) collected from two experimental fields, Bucium and Copou (North East of Romania). In general, the fresh skin of Cabernet and Merlot varieties has the highest flavonoids content followed by those obtained by oven-drying and freeze-drying process. A similar situation was observed in the other component of grape varieties like pulp and seeds meaning in the way that the flavonoid content in oven-dried samples were higher than that in the freeze-dried samples. In addition, from both varieties, Merlot collected from Bucium presented the highest content compared with Cabernet.

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Section: Resultscontrasting

confidence: 90%

Effect of Freeze-drying and Oven-drying Methods on Flavonoids Content in two Romanian Grape Varieties

Oprică¹,

Antohe²,

Verdes³

et al. 2019

Rev. Chim.

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“…The same finding was reported by Wong et al (2010); they indicated that freeze-dried samples of Alpinia species leaves can preserve antioxidant properties. Freeze-drying sample of leaves and berries of Cayratia trifolia or lakom also yields the highest antioxidant activity among vacuum-oven dried samples (Rabeta and Lin, 2015).…”

Section: Antioxidant Activity Assays (Dpph and Frap)mentioning

confidence: 93%

“…Dilution effects may be the reason for the low antioxidant activity, TPC, and total flavonoid content (TFC) of compounds. This low performance was attributed that the enzymes in fresh leaves were still active, and they can lead to enzymatic degradation (Rabeta and Lin, 2015). High moisture content in fresh samples can be an indicator of high water activity.…”

Section: Total Phenolic Content (Tpc)mentioning

confidence: 99%

Effects of different drying methods on the proximate composition and antioxidant activities of Ocimum basilicum leaves

Mahirah¹,

Rabeta²,

Antora³

2018

Food Res.

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Two different drying methods-freeze drying and vacuum oven drying were applied to Ocimum basilicum leaves to determine the proximate composition and antioxidant activities. The results were compared to fresh leaves of Ocimum basilicum. Each sample was prepared for determination in three different solvents-methanol, ethanol, and water have been used. The total phenolic content (TPC) and total flavonoid content (TFC) assays showed almost the same values in the freeze-dried sample of methanol extract as it exhibited the highest TPC and TFC values-54.46±1.24 mg gallic acid/g, 950±17.32 mg quercetin/g whereas the water extract showed only 26.23±0.63 mg gallic acid/g and 543.33±56.86 mg quercetin/g, respectively. On the contrary, fresh sample with water extract displayed the lowest TPC and TFC. In addition, the percent inhibition of 1,1diphenyl-2-2picrylhydrazyl (DPPH) free radical-scavenging assay ranged from 6.09± 0.47% in fresh sample with water extract to 92.60±0.58% in freeze-dried sample with methanol extract. The ferric reducing antioxidant power (FRAP) assay values ranged from 7.62±1.09 µmol FeII/g in fresh samples with water extract to 1,160.95±15.80 µmol FeII/g in freeze-dried sample with methanol extract. Both assays showed the same trend of result with the highest antioxidant activity observed in freeze-dried sample with methanol extract and the lowest from fresh samples with water extract.

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“…For this step, plant samples are usually freeze dried. It has been reported that non-thermally dried plants (lyophilization) result in higher flavonoids detection compared to thermal drying or fresh samples [87].…”

Section: Extraction Of Plant Flavonolsmentioning

confidence: 99%

“…Ramps were cleaned and stored in a -20 o C freezer until used for analysis. Ramps samples were lyophilized and pulverized for analysis because it was reported that this technique maximizes flavonols extraction [87].…”

Section: Plant Materialsmentioning

confidence: 99%

Characterization of Major Flavonols in Ramps (Allium tricoccum)

Dabeek¹

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Ramps (Allium tricoccum) is a traditional plant in the eastern Appalachian Mountains. Ramps have been used in Appalachian folk medicine for its health promoting roles in lowering blood pressure and cholesterol. Information on the chemical composition of bioactive components responsible for the potential health benefits are scarce. Analysis of the chemical composition in similar Allium plants showed that flavonols, induced similar health benefits. Therefore, the aim of this work is to characterize major flavonol conjugates in ramps. The secondary aim is to develop a preliminary quantification protocol. Flavonol glycosides were extracted in 50% methanol and 3% acetic acid. Characterization of flavonol glycosides was conducted using UHPLC-PDA-MS 2. For acid hydrolysis protocol, flavonol glycosides extracts or plant tissues were hydrolyzed by different concentrations of HCl. UHPLC-PDA was used to detect hydrolyzed flavonols. Characterization showed that the major putative flavonol glycosides were kaempferol sophoroside glucuronide, quercetin sophoroside glucuronide, and quercetin dihexoside. The different parts of the putative structures were preliminarily confirmed using 1 H and C 13 NMR analysis. The identified flavonol glycosides were concentrated in leaves. Preliminary quantification protocol showed that optimal hydrolysis conditions of flavonol conjugates was achieved by hydrolyzing the glycosides extract under 3M and 4M HCl, respectively, at 90 O C for two hours. To our knowledge, this work is the first to identify major flavonol glycosides content in ramps and develop a preliminary quantification protocol. This finding might explain the past usage of ramps as a medicinal plant. iii ACKNOWLEDGMENTS This work wouldn't be possible without the guidance and contribution of several people. First, I would like to express the deepest appreciation and gratitude to my committee chair, Dr. Melissa Ventura-Marra for her endless support during my educational journey. I am grateful for all the knowledge, experiences, passion, encouragement, and learning opportunities she provided. It was a great honor and pleasure to be a member of her research group. My words can't thank her enough for gifting me an unforgettable and invaluable journey. Also, I would like to extend my gratitude to my committee members Dr. Nikola Kovinich and Dr. Callee Walsh for their help on each step of the project. I have gained an enormous amount of knowledge in a new advanced field of research from their experiences. I appreciate their time and efforts on this project. My sincere thanks also go to Dr. Novruz Akhmedov for all the time, effort and expertise he provided on this work. His passion, assistance, and encouragement are invaluable. In a short period of time, he has provided a significant guidance and contribution on the project which are truly appreciated. I would also like to thank my research teammates for the help and all the insightful discussions. Also, I extend my appreciation to Denzil Blosser and Suzan Slider for all the help provided d...

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Effects of Different Drying Methods on the Antioxidant Activities of Leaves and Berries of Cayratia trifolia

Cited by 34 publications

References 18 publications

Effect of Freeze-drying and Oven-drying Methods on Flavonoids Content in two Romanian Grape Varieties

Effect of Freeze-drying and Oven-drying Methods on Flavonoids Content in two Romanian Grape Varieties

Effects of different drying methods on the proximate composition and antioxidant activities of Ocimum basilicum leaves

Characterization of Major Flavonols in Ramps (Allium tricoccum)

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