Effects of Different Doses of Taurine in the Glucose-Based Extender During Cryopreservation of Rainbow Trout (<i>Oncorhynchus Mykiss</i>) Semen

Ekici, Aygül; Baran, Alper; Yamaner, Güneş; Özdaş, Özen Banu; Sandal, Asiye İ̇zem; Güven, Erdoğan; Baltacı, M. Ali

doi:10.5504/bbeq.2012.0041

Cited by 23 publications

(20 citation statements)

References 23 publications

(27 reference statements)

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“…Taurine and hypotaurine are sulfuronic amino acids that have many physiological functions, including fat digestion, membrane stabilization, antioxidation, osmoregulation, modulation of ion flux, control of calcium homeostasis and are involved in the visual, neural, and muscular systems [ 70 , 71 ]. Moreover, some studies indicate that taurine is present in the reproductive tract [ 72 ] and can regulate the rate of ROS production by mitochondria, acting indirectly on oxidative stress, although its mechanism of action is still unclear [ 71 , 73 ]. As with other amino acids, there are effective and non-effective results on post-thaw fish sperm quality when cryopreservation medium was supplemented with taurine.…”

Section: Fish Sperm Antioxidant System and Supplementation: Classimentioning

confidence: 99%

Antioxidants in Fish Sperm and the Potential Role of Melatonin

2020

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In recent years, the effects of novel antioxidants have played an important role in the research focusing on fish cell protection. As food demand grows, aquaculture production becomes more intensive, and fish are more exposed to oxidative stress conditions, like high densities, temperature shifting, frequent fish handling and samplings, and prophylactic or disease treatments, which expose fish to a different environment. Particularly in reproduction, germ cells lose antioxidant capacity with spermatogenesis, as spermatozoa are more prone to oxidative stress. Antioxidants have been used in a variety of fish physiological problems including in reproduction and in the establishment of cryopreservation protocols. From the most used antioxidants to natural plant food and herbs, and endogenously produced antioxidants, like melatonin, a review of the literature available in terms of their effects on the protection of fish spermatozoa is presented here in a classified structure. Several direct and indirect approaches to improve gamete quality using antioxidants administration are mentioned (through feed supplementation or by adding in cryopreservation media), as well as factors affecting the efficiency of these molecules and their mechanisms of action. Special attention is given to the unclear melatonin pathway and its potential scavenger activity to prevent and counteract oxidative stress damage on fish spermatozoa.

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Section: Fish Sperm Antioxidant System and Supplementation: Classimentioning

confidence: 99%

Antioxidants in Fish Sperm and the Potential Role of Melatonin

2020

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“…taurine, hypotaurine, proline, glutamine, glycine, histidine, cysteine) to extenders reduced sperm damage and improved post-thaw motility (Chen et al 1993;Sanchez-Partida et al 1997;Kundu et al 2001;Bucak et al 2007). Recently, studies about benefit from antioxidant property and addition to extenders of amino acids have been performed in different fish species, such as Acipenser baerii and A. ruthenus by Stejskal et al (2008); Dicentrarchus labrax and Sparus aurata by Cabrita et al (2011); Oncorhynchus mykiss by Ekici et al (2012) and Cyprinus carpio by Ogretmen et al (2015).…”

Section: Introductionmentioning

confidence: 99%

Retracted: Influence of Glutamine Supplementation on Motility and Fertilization Success of Frozen–Thawed Persian Sturgeon (Acipenser persicus) Sperm

Aramli

Golshahi

Nazari³

et al. 2016

Reprod Domestic Animals

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Amino acids have an important biological role for the prevention of cell damage during cryopreservation. The aim of this study was to investigate the effects of glutamine on post-thaw sperm motility and fertilization success in the Persian sturgeon (Acipenser persicus). Sperm collected from six fish was cryopreserved in extenders containing different glutamine concentrations (2.5, 5 and 10 mm). Sperm samples diluted at the ratio of 1 : 1 using the extenders were subjected to cryopreservation. After dilution, the sperm suspensions were sucked into 250-μl straws; the straws were placed on the tray, frozen in nitrogen vapour and plunged into liquid nitrogen. Then, sperm were thawed in a water bath at 40°C for 5 s and used for analysis. Our results revealed that an increase in the concentration of glutamine caused a significant increase in the motility percentage, curvilinear velocity (VCL) and also fertilization success in the Persian sturgeon (p < 0.05). Comparing all concentrations of glutamine, the best concentration for sperm motility and fertilization rate was 10 mm. In addition, higher post-thaw motility percentage, VCL, and fertilization and hatching rates were obtained with the extender at the concentration of 10 mm (p < 0.05). The findings of this study showed that glutamine was of greater benefit to Persian sturgeon sperm motility during frozen-thawed process.

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“…In cryopreservation experiment, Extender 1, contained 10% DMSO solution extended with 300 mM glucose solution was used previously (Ekici et al 2012) with high motility after thawing as found in this study. Our results also showed it as the most suitable extender for washing.…”

Section: Discussionmentioning

confidence: 99%

“…Straws were frozen at 2.5 cm above the level of liquid nitrogen, with a holding time of 10 min. Straws (0.25 mL) were stored in liquid nitrogen for 4 months (long term) (Ekici, Baran, Yamaner, Özdaş, Sandal, Güven & Baltacı ).…”

Section: Methodsmentioning

confidence: 99%

Reducing bacterial density in the semen of rainbow trout (Oncorhynchus mykiss) by applying gradient centrifugation and swim-up washing methods

Ercan

Ekici

2015

Aquac Res

Self Cite

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The objective of this study was to demonstrate the effectiveness of disinfection procedures to reduce bacterial load of rainbow trout, Oncorhynchus mykiss (Walbaum 1792), semen. Fresh semen was obtained from 3–4‐year‐old male species by abdominal sampling of sperm into pre‐cooled test tubes. After sperm cryopreservation and thawing, experiments were accomplished at 4–9°C. ‘Swim‐up’ and gradient centrifugation were used as a sperm washing method with commercial kits. Phosphate buffered saline was also used as washing solution. Bacterial growth tests were employed before and after washing the semen samples. Samples were inoculated on tryptic soy agar (TSA), modified Anacker and Ordal agar (MAOA) as well as brain heart infusion (BHI) agar. After using ‘swim‐up’ method for washing the semen, many bacterial colonies were observed. However, after semen washing with gradient centrifugation, lower bacterial growth was observed on TSA, MAOA and BHI. Some motile (40%) spermatozoa were obtained doing gradient washing procedure. Although sperm motility was not satisfactory, apparently the gradient centrifugation method reduced bacterial contamination as known from the mammalians.

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Effects of Different Doses of Taurine in the Glucose-Based Extender During Cryopreservation of Rainbow Trout (Oncorhynchus Mykiss) Semen

Cited by 23 publications

References 23 publications

Antioxidants in Fish Sperm and the Potential Role of Melatonin

Antioxidants in Fish Sperm and the Potential Role of Melatonin

Retracted: Influence of Glutamine Supplementation on Motility and Fertilization Success of Frozen–Thawed Persian Sturgeon (Acipenser persicus) Sperm

Reducing bacterial density in the semen of rainbow trout (Oncorhynchus mykiss) by applying gradient centrifugation and swim-up washing methods

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