Effects of Different Doses of Low Power Continuous Wave He–Ne Laser Radiation on Some Seed Thermodynamic and Germination Parameters, and Potential Enzymes Involved in Seed Germination of Sunflower (Helianthus annuus L.)

Abstract

In this study, water-soaked seeds of sunflower were exposed to He-Ne laser irradiation of different energies to determine whether or not He-Ne laser irradiation caused changes to seed thermodynamic and germination parameters as well as effects on the activities of germination enzymes. The experiment comprised four energy levels: 0 (control), 100, 300 and 500mJ of laser energy and each treatment replicated four times arranged in a completely randomized design. The experimentation was performed under the greenhouse conditions in the net-house of the Department of Botany, University of Agriculture, Faisalabad. The seed thermodynamic parameters were calculated according to seed germination thermograms determined with a calorimeter at 25.8°C for 72h. Various thermodynamic parameters of seed (ΔH, (ΔS)(e), (ΔS)(c), (ΔS)(e) /Δt and (ΔS)(c) /Δt) were affected significantly due to presowing laser treatment. Significant changes in seed germination parameters and enzyme activities were observed in seeds treated with He-Ne laser. The He-Ne laser seed treatment resulted in increased activities of amylase and protease. These results indicate that the low power continuous wave He-Ne laser light seed treatment has considerable biological effects on seed metabolism. This seed treatment technique can be potentially employed to enhance agricultural productivity.

Changes in the germination process and growth of pea in effect of laser seed irradiation

“…We know at present that laser stimulation enables the seed cell to absorb and store radiant energy, which is then converted to chemical energy as a useable form in subsequent growth processes (Jamil et al, 2013). The above observation is substantiated by studies that confirmed the positive effects of laser radiation on enzyme activity at the early stages of germination (Chen et al, 2005b;Perveen et al, 2010;Rubinov, 2003).…”

Impact of presowing laser irradiation of seeds on sugar beet properties

“…Researches on the effect of laser biostimulation on plants mainly concern seed germination, plant emergence, yield quantity and in rarer cases yield quality (G³adyszewska, 2011; 298 E. SACA£A et al Days after sowing Ivanova, 1999;Muszyñski and G³adyszewska, 2008;Osman et al, 2009;Perveen et al, 2010). Examinations usually are limited to one harvest at the end of the vegetation period (Koper et al, 1996, Rochalska et al, 2008, Rybiñski and Garczyñski, 2004.…”

“…One of these methods is laser pretreatment of crops seeds. The literature data indicate that laser irradiation increases seed germination and improves plant growth and metabolism (Chen et al, 2005a, b;Drozd and Szajsner, 2007;Perveen et al, 2010;Podleoeny 2002). Numerous studies have proved that laser irradiation improves plant resistance to some environmental stresses (Chen, 2008;Qi et al, 2000;Qiu et al, 2008).…”

Enhancement in growth and yield of mushroom using magnetic field treatment

“…In this regard, the agricultural scientists and food technologists are trying to search for methods which must be proficient, ecofriendly and inexpensive. Biological, chemical and physical treatments are being used to obtain higher growth and yield (AEwintal et al, 2010;Hernandez-Aguilar et al, 2009;Perveen et al, 2010). Chemical treatments were found to be effective for enhancing the growth and yield, but might be detrimental at later stages of development (Dao-liang et al, 2009).…”