Effects of different diluents, cryoprotective agents, and freezing rates on sperm cryopreservation in Epinephelus akaara

Ahn, Jae Yeon; Park, Jung Yeol; Lim, Han Kyu

doi:10.1016/j.cryobiol.2018.06.003

Cited by 40 publications

(28 citation statements)

References 16 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…More than 200 studies on the cryopreservation of fish milt have been developed globally (Torres et al 2016). However, post‐thaw sperm viability and quality are highly dependent on numerous biological and methodological factors, such as the origin and nutritional status of the fish (Cabrita et al 2014), type of cryoprotective agents (CPAs: permeating and nonpermeating) and their concentration (Judycka et al 2018), freezing/thawing rate, equilibrium time (Ahn et al 2018), packaging system, membrane stabilizers, and antioxidants (Fig ueroa et al 2017, 2018). In addition, the methodologies need to be adapted for each species (Pan et al 2008; Martínez‐Páramo et al 2017; Yang et al 2017; Bernáth et al 2018).…”

mentioning

confidence: 99%

Effect of Cryopreservation and Packaging System on Sperm Motility and Fertility of Striped Catfish

et al. 2021

View full text Add to dashboard Cite

The Orinoco Striped Catfish Pseudoplatystoma orinocoense is a highly valued commercial‐capture species in Colombia. Although this species was considered endangered and is now in a recovery situation, there are still no cryopreservation studies for its milt. Therefore, the objective of this study was to evaluate the effects of different cryopreservation media and two packaging systems on the sperm motility and fertilization capacity of Striped Catfish. A first experiment was conducted to test the effects of two permeating cryoprotectants (10% dimethyl sulfoxide and 12% methanol) combined with glucose at two concentrations (5.5% and 10%) and with two membrane stabilizers (12% egg yolk and 5% whole milk powder) on the post‐thaw motility of milt samples that were packed into 0.5‐mL straws. The cryopreservation media with the best results in the first experiment were selected for a second experiment in which we evaluated 5‐mL macrotubes as a packaging system. In this case, the response variables were sperm motility and fertility rate. In both experiments, milt with sperm motility that exceeded 90% was diluted at a 1:6 (volume basis) ratio in each of the two cryopreservation media. The diluted samples were packed, equilibrated (for 10 min), then frozen in a nitrogen‐vapor dry shipper for 30 min, and finally stored in liquid nitrogen until they were evaluated. Each factor and its interactions showed a significant effect (P < 0.001) on post‐thaw sperm motility in experiment 1. The best protection during cryopreservation was obtained when 12% methanol supplemented with 5.5% glucose was used as a permeating cryoprotectant, yielding an average motility of 33.3 ± 5.1% (mean ± SD) and 64 ± 5.4% in the 0.5‐ and 5‐mL packaging systems, respectively. Similarly, the highest fertility rate (85%) was recorded under this treatment, with no significant difference (P > 0.05) from the fresh milt. Therefore, Striped Catfish milt can be cryopreserved in 5‐mL macrotubes by using 12% methanol and 5.5% glucose, without the addition of membrane stabilizers.

show abstract

mentioning

confidence: 99%

Effect of Cryopreservation and Packaging System on Sperm Motility and Fertility of Striped Catfish

et al. 2021

View full text Add to dashboard Cite

show abstract

“…Enhanced capacity on controlling freezing temperatures and freezing rates in a dry shipper would generate a wide range of applications in semen cryopreservation of other freshwater and marine fish species that require different optimal freezing rates ranging from 5 to 93°C/min (He & Woods, ; Hu, Yang, & Tiersch, ; Kang et al, ; Liu et al, ; Magnotti et al, ; Torres, Hu, & Tiersch, ; Velasco‐Santamaría et al, ). A wide range of optimal freezing rates for semen cryopreservation of fish is possibly explained by unique characteristics of spermatozoa among species, concentrations of cryoprotectants, equilibration time, final temperatures prior to plunging in liquid nitrogen and their associated interactions (Ahn, Park, & Lim, ; Koh et al, ; Routray et al, ).…”

Section: Discussionmentioning

confidence: 99%

Development of cryopreservation methods for silver barb ( Barbodes gonionotus , Bleeker) spermatozoa by manipulation of cooling rates in dry shipper

et al. 2019

View full text Add to dashboard Cite

The aim of this study was to develop a simple cryopreservation protocol for silver barb, Barbodes gonionotus, semen using a dry shipper. Freezing rates within the upper and lower chambers of dry shipper were recorded for 14 days post liquid nitrogen loading (dpl). To regulate freezing rates, straws (250 and 500 µl) wrapped with various insulators (polystyrene foam box, oxygen tube, silicone tube and electric wire) were frozen within the upper chamber. Straws containing semen diluted with Calcium‐free Hank's Balanced Salt Solution (Ca‐F HBSS) and 10% dimethyl sulphoxide were cryopreserved with or without insulators. Appropriate protocols were selected based on sperm quality during a 45‐day cryostorage. The upper chamber had potential as a freezing chamber within 9 dpl due to no significant (p > 0.05) change in freezing rates. High percentages of sperm motility and viability (p < 0.05) were observed when 250 µl straws with silicone tube (T4) frozen for 5 min, non‐insulated 500 µl straws (T9) and 500 µl straws with polystyrene foam box (T12) frozen for 1–5 min, having freezing rates of 43.1 ± 1.3, 71.3 ± 1.4 and 14.7 ± 0.4°C/min respectively. Dry shipper can be used as a freezing tool to cryopreserve silver barb semen.

show abstract

“…In addition, because the types and concentrations of cryoprotective agents (CPAs) and diluents can cause remarkable differences in cryopreservation results, basic information on cryopreservation of fish sperm is necessary (Lee et al, 2021). CPAs play a role in alleviating and regulating conditions unfavorable to cell survival, such as concentration of intracellular electrolytes, increase in osmolality, and formation of ice crystals inside and outside cells, which are generated during the freezing of cells (Ahn et al, 2018). The diluted solution actively helps in the survival of the sperm after freezing and thawing and prevents the sperm from being activated by osmotic shock, with osmolality similar to that of the semen used (Kho & Kang, 2003;Zidni et al, 2020).…”

Section: Introductionmentioning

confidence: 99%

Effect of diluent variation on cryopreservation of large yellow croaker Larimichthys crocea

Lim¹,

Zidni²,

Lee³

et al. 2021

Fish Aquat Sci

Self Cite

View full text Add to dashboard Cite

The aim of this research was to investigate different factors, including cryoprotective agents (CPAs), diluents, dilution ratios, equilibrium times, freezing rates, and thawing methods to optimize cryopreservation protocols for large yellow croaker (Larimichthys crocea). The parameters evaluated were sperm motility, sperm activity index (SAI), survival rate, and DNA damage. Different types of CPAs, such as dimethyl sulfoxide (DMSO), propylene glycol (PG), ethylene glycol (EG), methanol, and glycerol, were tested for sperm preservation. The highest motility, SAI, and survival rate were observed when EG was used. Different diluents such as Stein's solution, Hank's balanced salt solution, marine fish Ringer's solution, artificial seminal plasma (ASP) of small yellow croaker, and Cortland solution were investigated. The highest post-thaw motility was observed upon using ASP as the diluent. Different concentrations of EG were then mixed with ASP to identify the optimal EG concentration. Experimental results showed that the motility (70.33 ± 1.20%), SAI (5), and survival rate (78.30 ± 0.42%) of post-thaw sperm were optimum when 10% EG and ASP were used as the CPA and diluent of cryopreservation, respectively. Post-thaw sperm motility was high at equilibration times below 150 s and at an optimum dilution ratio of 1:1 (sperm: CPA + diluent) and was not significantly different compared with fresh sperm motility. The freezing rate was found to be slow below -10℃/min. The thawing temperature of 45℃ was identified as ideal. The percentage of tail DNA in post-thaw sperm at 10% EG and ASP was also investigated and was found to have more significant DNA damage than that in fresh sperm but significantly lower damage than that in post-thaw sperm at EG concentrations of 5%, 15%, and 20% (p < 0.05). The cryopreservation protocols obtained in this study will be useful in large yellow croaker hatcheries.

show abstract

Effects of different diluents, cryoprotective agents, and freezing rates on sperm cryopreservation in Epinephelus akaara

Cited by 40 publications

References 16 publications

Effect of Cryopreservation and Packaging System on Sperm Motility and Fertility of Striped Catfish

Effect of Cryopreservation and Packaging System on Sperm Motility and Fertility of Striped Catfish

Development of cryopreservation methods for silver barb ( Barbodes gonionotus , Bleeker) spermatozoa by manipulation of cooling rates in dry shipper

Effect of diluent variation on cryopreservation of large yellow croaker Larimichthys crocea

Contact Info

Product

Resources

About