Effects of Different Dietary β-Glucan Levels on Antioxidant Capacity and Immunity, Gut Microbiota and Transcriptome Responses of White Shrimp (Litopenaeus vannamei) under Low Salinity

Qiao, Yanbing; Zhou, Li; Qu, Yayu; Lu, Kunyu; Han, Fenglu; Li, Erchao

doi:10.3390/antiox11112282

Cited by 21 publications

(21 citation statements)

References 58 publications

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“…Here, β-1,3-glucan supplementation did affect the intestinal microbiota and significantly increased the microbial diversity in white shrimp. Similar results were also observed in tilapia ( 21 ), carp ( Cyprinus carpio ) ( 37 ) and white shrimp from a recent study ( 22 ). Although the composition of the intestinal microbial community can be dramatically affected by the diets, the core microbiota is essentially stable in the host ( 38 ).…”

Section: Discussionsupporting

confidence: 87%

“…Total number of edges 3847 4743 also observed in tilapia (21), carp (Cyprinus carpio) (37) and white shrimp from a recent study (22). Although the composition of the intestinal microbial community can be dramatically affected by the diets, the core microbiota is essentially stable in the host (38).…”

Section: Discussionmentioning

confidence: 78%

“…Multitudinous evidence has confirmed that a vital role of intestinal microbiota in the intestinal health of the host, and β-glucan, a complex polysaccharide, is authorized to possess various health-promoting properties through intestinal microbiota regulation ( 17 – 19 ). Several studies explored the effects of β-glucan on the intestinal microbiota of aquatic animals including common carp ( 20 ), tilapia ( 21 ), rainbow trout ( 1 ), and white shrimp ( 22 ), mainly focusing on microbial composition. Undoubtedly, the number and richness of species are essential elements for a microbial ecosystem, while complex interspecific interactions are crucial to maintaining intestinal microbiota homeostasis ( 23 ).…”

Section: Introductionmentioning

confidence: 99%

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Dietary supplementation of β-1, 3-glucan improves the intestinal health of white shrimp (Litopenaeus vannamei) by modulating intestinal microbiota and inhibiting inflammatory response

et al. 2023

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The phenomenon of intestinal dysfunction is widely observed in white shrimp (Litopenaeus vannamei) culture, and β-1,3-glucan has been confirmed to be beneficial in intestinal health with a lack understanding of its underlying mechanism. Proteobacteria, Firmicutes, and Actinobacteria served as the predominant phyla inhabiting the intestine of white shrimp, whilst a significant variation in their proportion was recorded in shrimp fed with basal and β-1,3-glucan supplementation diets in this study. Dietary supplementation of β-1,3-glucan could dramatically increase the microbial diversity and affect microbial composition, concurrent with a notable reduction in the ratio of opportunistic pathogen Aeromonas, gram-negative microbes, from Gammaproteobacteria compared to the basal diet group. The benefits for microbial diversity and composition by β-1,3-glucan improved the homeostasis of intestinal microbiota through the increase of specialists’ number and inhibition of microbial competition caused by Aeromonas in ecological networks; afterward, the inhibition of Aeromonas by β-1,3-glucan diet dramatically suppressed microbial metabolism related to lipopolysaccharide biosynthesis, followed by a conspicuous decrease in the intestinal inflammatory response. The improvement of intestinal health referred to the elevation in intestinal immune and antioxidant capacity, ultimately contributing to the growth of shrimp fed β-1,3-glucan. These results suggested that β-1,3-glucan supplementation improved the intestinal health of white shrimp through the modulation of intestinal microbiota homeostasis, the suppression of intestinal inflammatory response, and the elevation of immune and antioxidant capacity, and subsequently promoted the growth of white shrimp.

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Section: Discussionsupporting

confidence: 87%

Section: Discussionmentioning

confidence: 78%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Dietary supplementation of β-1, 3-glucan improves the intestinal health of white shrimp (Litopenaeus vannamei) by modulating intestinal microbiota and inhibiting inflammatory response

et al. 2023

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“…As the intestine of L. vannamei is not just involved in nutrient absorption but also related to immune capacity, the changes in the composition of the microbiota may cause diseases and increase mortality [ 43 ]. Previous studies have reported that environmental stress [ 44 , 45 ] and prebiotics [ 46 , 47 ] could induce a significant impact on the α-diversity of intestinal microbiota in shrimp. In this study, the alpha diversity of intestine microbiota was changed under the stress of low salinity and antibiotics.…”

Section: Discussionmentioning

confidence: 99%

Effects of Sulfamethoxazole and Florfenicol on Growth, Antioxidant Capacity, Immune Responses and Intestinal Microbiota in Pacific White Shrimp Litopenaeus vannamei at Low Salinity

Chen

Zhou

et al. 2023

Antibiotics

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Antibiotic residue may pose a serious risk to aquaculture, and the culture of Litopenaeus vannamei in a low-salinity environment is a growing trend over the world. Here, we aimed to understand the combined effect of low salinity and sulfamethoxazole (SMZ) and florfenicol (FLO) antibiotics on L. vannamei. The growth performance, immune functions, antioxidant capacity and intestinal microbiota were investigated. Compared with the control group, the weight gain and survival rate significantly decreased (p < 0.05) in shrimp after they were exposed to low-salinity (salinity 3) water and the mixture of antibiotics and low-salt conditions for 28 days. The antioxidant activities of SOD and T-AOC, shown at low salinity and in the higher concentration of the SMZ treatment group (SMZH), were significantly decreased, while the GST activity was significantly increased in each treatment group in comparison with the control group. The expression of immune-related genes, including TOLL, LvIMD, PPO and HSP, in the low concentration of the SMZ treatment group (SMZL) was higher than that in the other groups. The diversity of intestine microbiota was disturbed with a lower Shannon index in the low-salinity and SMZH groups, and a higher Simpson index in the SMZH group. Proteobacteria, Actinobacteria and Bacteroidetes were the dominant phyla in the gut of L. vannamei. At the genus level, Microbacterium, Shewanella, Aeromonas, Acinetobacter, Gemmobacter, Paracoccus and Lysobacter were significantly decreased in the low-salinity group. However, the abundance of opportunistic pathogens belonging to the genus Aeromonas in the FLO group was increased. The predicted microbe-mediated functions showed that the pathway for “amino acid metabolism” and “replication and repair” was significantly inhibited in both the low-salinity and antibiotic-exposed groups. All the findings in this study indicate that the combined effect of antibiotics and low salinity on L. vannamei negatively impacted the physiological and intestinal microbiota functions.

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“…Hepatopancreas are important detoxigenic organs and the first barrier against the interference of the whole organism (Pawert et al, 1996;Opstvedt et al, 2000;. However, only a few studies have combined molecular and biochemical procedures to describe the relationship between the transcription of specific immune and antioxidant genes and their activity (Qiu et al, 2018;Chen et al, 2021;Qiao et al, 2022), and the response mechanism of shellfish to residual chlorine stress is also rarely studied.…”

Section: Introductionmentioning

confidence: 99%

Transcriptome reveals the immune and antioxidant effects of residual chlorine stress on Cyclina sinensis

Wang¹,

Ren²,

Li³

et al. 2023

Front. Mar. Sci.

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Residual chlorine is a common by-product of warm drainage in coastal nuclear power plants. when accumulating to some limit, it may threaten marine ecosystem especially for benthic clam. However, there are few studies on the molecular mechanisms related to immunity and antioxidant of residual chlorine stress on clams. In this study, the clam (Cyclina sinensis) was exposed for 96 h at different concentrations (0, 50, 100, 150, 200, 250, 300, 350, 400, 450 and 500 mg/L) of residual chlorine to observe its mortality, measure the activity of antioxidant and immune-related enzymes, and analyses the gene expression level in the hepatopancreas by using the transcriptome sequencing. The results showed that the mortality rate increased with the increase of stress time and concentration, and the mortality rate in the 400, 450 and 500 mg/L groups reached 100% at 96 h. The tolerance to residual chlorine of C. sinensis decreased with the increase of chlorine dioxide concentration, and the LC50 of 96 h was 217.6 mg/L by linear regression method. After residual chlorine stress, the activity of antioxidant-related enzymes (T-AOC and SOD) in the hepatopancreas showed a trend of first increase and then decrease with the extension of stress time. The immune-related enzyme activities of AKP and LZM showed a downward trend between 0 and 96 h, while the ACP enzyme activity showed a trend of first rising and then decreasing. Transcriptome analysis showed that residual chlorine stress significantly changed the expression levels of immune-related molecules associated with signal transduction, prophenoloxidase cascade, cell apoptosis and pattern recognition protein/receptor. Moreover, glutathione S-transferase (GST), heat shock protein (HSP) and other antioxidant-related genes were significantly affected under residual chlorine stress. This study provided valuable information for understanding the effects of residual chlorine stress on survival, physiological metabolism and molecular mechanisms of immune and antioxidant functions of C. sinensis.

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Effects of Different Dietary β-Glucan Levels on Antioxidant Capacity and Immunity, Gut Microbiota and Transcriptome Responses of White Shrimp (Litopenaeus vannamei) under Low Salinity

Cited by 21 publications

References 58 publications

Dietary supplementation of β-1, 3-glucan improves the intestinal health of white shrimp (Litopenaeus vannamei) by modulating intestinal microbiota and inhibiting inflammatory response

Dietary supplementation of β-1, 3-glucan improves the intestinal health of white shrimp (Litopenaeus vannamei) by modulating intestinal microbiota and inhibiting inflammatory response

Effects of Sulfamethoxazole and Florfenicol on Growth, Antioxidant Capacity, Immune Responses and Intestinal Microbiota in Pacific White Shrimp Litopenaeus vannamei at Low Salinity

Transcriptome reveals the immune and antioxidant effects of residual chlorine stress on Cyclina sinensis

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