Effects of different dietary protein sources on the immunological and physiological responses of marron, Cherax cainii (Austin and Ryan, 2002) and its susceptibility to high temperature exposure

Fermented Animal Source Protein as Substitution of Fishmeal on Intestinal Microbiota, Immune-Related Cytokines and Resistance to Vibrio mimicus in Freshwater Crayfish (Cherax cainii)

“…This result is in agreement with a previous study on marron fed PBM supplemented with black soldier fry insect meal and found no variations in growth performance (Foysal et al, 2019a). Past studies have also demonstrated that FM ingredients can be replaced completely by using other protein sources without negative effects on growth but compromising biochemical and immunological responses of marron (Fotedar, 2004;Saputra et al, 2019). Whilst there is little literature about the application of fermented animal protein source diets in crustacean aquaculture, there are some studies with fermented plant source diets.…”

“…prevented the suppression of immune function triggered by soybean meal in white shrimp, Litopenaeus vannamei. However, the results of our previous research indicated that replacing FM with nonfermented PBM leads to no significant variation in microvilli number in the midgut of marron (Saputra et al, 2019).…”

“…In crustacean haemocytes, antimicrobial effector cells play an important role in regulating physiological and immunological functions by acting in both adoption of the cellular immunity and supply of the humoral immunity (Liu et al, 2009;Hong et al, 2017;Saputra et al, 2019). The THC and DHC in the circulatory system of crustaceans may be easily affected by intrinsic factors and extrinsic factors including heavy metals, pathogens and pesticides (Cheng and Chen, 2000;Yao et al, 2008).…”

“…Six marron from each control and FPBM groups (one from each tank) were sampled to dissect and extract the mid intestine for scanning electron microscopy (SEM) analysis. The SEM samples were prepared following the method described in Saputra et al (2019). In short, the collected samples were soaked in 3% glutaraldehyde in 0.1 M cacodylate buffer overnight.…”

“…It has been reported that a omnivorous freshwater Australian crayfish, marron (Cherax cainii) can be successfully cultured using poultry by-product meal (PBM), feather meal, lupin meal, or meat and bone meal as the main protein source (Saputra et al, 2019). In that study, it was found that PBM as an alternative protein source to FM increased the total haemocyte count, microvillus length and susceptibility to high temperature (30 • C) exposure (Saputra et al, 2019). In addition, PBM supplemented with black soldier fly (Hermetia illucens) meal revealed a higher bacterial activity and gene function correlated to biosynthesis of protein and energy in marron (Foysal et al, 2019a,b).…”