Effects of different dietary levels of vitamin E on the ovarian development and breeding performances of <i>Clarias batrachus</i> (Linnaeus)

Abstract

To observe the effects of vitamin E on the ovarian development and breeding performances of Clarias batrachus an experiment was conducted in two phases. The first phase concentrated on studying the effects of vitamin E on ovarian development and the second phase on breeding performances. Eighty female C. batrachus broodfish were stocked in 8 cisterns (2.38 × 1.45m 2 each) divided into 4 treatments each having two replicates. Each of the cistern was stocked with 10 females. The fish were fed with feed having different levels of vitamin E viz. 0 mg (served as control), 50 mg, 100 mg and 200 mg vitamin E/kg feed for 3 months to study the effects of vitamin E on the growth and ovarian development of C. batrachus broodfish. It was found that growth in terms of body weight of fish fed with 200 mg vitamin E/kg feed (under T 4 ) was higher, while 50 mg vitamin E/kg feed (under T 2 ) gave the poorest result. There was no significant difference among different treatments. Gonadosomatic index and fecundity were highest in fish treated with 100 mg vitamin E/kg feed (under T 3 ), but there was no significant difference among the treatments. After rearing for 3 months fish were used for induction of breeding to study breeding performance. A pituitary gland (PG) dose of 100 mg/kg body weight was used in all treatments. Ovulation rate was 100% in females of all treatments. In case of fertilization and hatching rate the highest result (88.33±2.51 and 82.33±3.05 respectively) was obtained in T 2 (50 mg vitamin E / kg feed), but there was no significant difference between T 2 and T 3 . The overall result of this experiment indicates that 50 mg vitamin E/kg feed is the best dose for the breeding performance of female C. batrachus broods.

Optimizing the gonadal performance of broodstock Helostoma temminckii with addition of enrichment ingredients in feed

“…Stated that nutrition in broodstock feed can affect gonadal development, fecundity, and embryo development [18]. This was in line with the results of the study where the addition of enriching ingredients in feed such as glutathione and vitamin E resulted in the highest GSI among other treatments.…”

Effect of dietary vitamin E on the sperm quality of turbot (Scophthalmus maximus)

“…Relevant studies have been carried out on a number of fish species such as ayu (Takeuchi et al, 1981), goldfish (Kashani et al, 2012), carp (Taati et al, 2010), Nile tilapia (Gammanpila et al, 2007), black sea trout (Serezli et al, 2010), guppy (Mehrad and Sudagar, 2010), and zebrafish (Mehrad et al, 2012), which suggested that dietary Ve can improve the reproduction of broodstocks and survival rate of larvae. In female fish, it has been widely observed that dietary Ve could improve diverse reproductive performance such as the number of spawners (Areechon et al, 2003), ovarian growth rate (Watanabe and Takashima, 1977;Gupta et al, 1987;Roy and Mollah, 2009), vitellogenesis (Kanazawa, 1985;Santiago and Gonzal, 2000), and egg quality (Izquierdo et al, 2001;Kashani et al, 2012).…”

Effects of vitamin E on growth and maturation in the Asian catfish (Clarias macrocephalus) at puberty

“…The maximum GSI values for either male or female C. macrocephalus in the present study were elicited at 215-216.2 mg•kg -1 . In Clarias batrachus, the highest GSI was observed in the 100 mg•kg -1 group, followed by 200, 0, and 50 mg•kg -1 (Roy and Mollah, 2009). In goldfish Carassius auratus, 100 mg•kg -1 VE was reported suitable for stimulating the development of gonads and spawning (Sanchai-Sutjaritvongsanon, 1987).…”