Effects of different culture conditions on biological potential and metabolites production in threePenicilliumisolates

Abstract

The genus Penicillium is well known for its importance in drug and food production.Certain species are produced on an industrial scale for the production of antibiotics (e.g. penicillin) or for insertion in food (e.g. cheese). In the present work, three Penicillium species, part of the natural mycobiota growing on various food products were selected-P. ochrochloron, P. funiculosum and P. verrucosum var. cyclopium. The objective of our study was to value these species from the point of view of production of bioactive metabolites. The species were obtained after inoculation and growth in Czapek and Malt media. Both mycelia and culture media were analyzed to monitor the production of different metabolites by each fungus and their release to the culture medium. The concentrations of sugars, organic acids, phenolic acids and tocopherols, were determined. Antioxidant activity of the phenolic extracts was evaluated, as also the antimicrobial activity of phenolic acids, organic acids and tocopherols extracts.Rhamnose, xylose, fructose and trehalose were found in all the mycelia and culture media; the prevailing organic acids were oxalic and fumaric acids, and protocatechuic and p-hydroxybenzoic acids were the most common phenolic acids; γ-tocopherol was the most abundant vitamin E isoform. Generally, the phenolic extracts corresponding to the mycelia samples revealed higher antioxidant activity. Concerning the antimicrobial activity there were some fluctuations, however all the studied species revealed activity against the tested strains. Therefore, the in vitro bioprocesses can be an alternative for the production of bioactive metabolites that can be used by pharmaceutical industry.

Screening of Dominant Cellulose-Degrading Microbe in Humus and Optimisation of its Enzyme Producing Conditions and Application Optimization of Enzyme Production Conditions and Evaluation of Efficient Straw Degradation by Penicillium oxalicum

“…Penicillium oxalicum can be isolated from various environments, including tropical commodities, food, air, and soil (Gupta et al, 2020;Tian et al, 2018). In recent years, P. oxalicum has been increasingly studied as a degrading hazardous microorganism (Tian et al, 2018;Wang et al, 2021;Ren et al, 2021) and researchers have been interested in it primarily as a popular cellulase-producing mold and polymers such as rhamnose, xylose, fructose, and trehalose were found in the mycelium and culture medium of P. oxalicum (Reis et al, 2015). Studies on cellulase production by P. oxalicum fall under three main categories.…”

Aspectos Ambientais Da Região Do Vale Do Rio Araguaia No Estado De Goiás –Brasil

“…Considerando os procedimentos de Reis et al, (2013a) e Reis et al, (2013b), foi criada uma técnica de extração em microescala baseada em solventes orgânicos para o manitol em corpos de frutificação de fungos previamente desidratados, para os quais cada Espécie separadamente usando um almofariz com pistilo, e foi submetido a uma digestão alcoólica pesando um grama de tecido adicionando 3 a 4 mL de álcool a 80% dependendo de quão fibroso parecia o concentrado, foi perfeitamente misturado e agitado a cada 30 minutos deixando os próximos 30 descansarem por uma hora e meia. Após esse período, as amostras foram colocadas em um evaporador rotativo até atingirem o ponto de ebulição por 5 minutos e imediatamente lavadas três vezes, filtradas com whatman nº 4 e 5 mL de éter etílico consecutivamente.…”

Agrotóxicos Na Agricultura: Consequências Toxicológicas E Ambientais

“…Considerando os procedimentos de Reis et al, (2013a) e Reis et al, (2013b), uma técnica de extração em micro-escala baseada em solventes orgânicos foi criada para o manitol em corpos de frutificação de fungos previamente desidratados, para os quais cada Espécie separadamente usando um almofariz com pistilo, e foi submetido a uma digestão alcoólica pesando um grama de tecido adicionando 3 a 4 mL de álcool a 80% dependendo de quão fibroso parecia o concentrado, foi perfeitamente misturado e agitado a cada 30 minutos deixando o seguinte descansar.…”