Effects of different alcohol concentrations on the development of apical periodontitis in rats

Dal‐Fabbro, Renan; Marques-de-Almeida, Melyna; Cosme‐Silva, Leopoldo; Capalbo, Letícia Cabrera; Ervolino, Edílson; Cintra, Luciano Tavares Ângelo; Gomes‐Filho, João Eduardo

doi:10.1016/j.archoralbio.2019.104538

Cited by 10 publications

(19 citation statements)

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“…Ethanol harmful effects in the oral cavity seem to be proportional to the alcohol-exposure pattern [ 7 , 12 , 13 ]. Enzymatic induction, immune modifier, as well as cementum formation and osteoblast activity reduction have been suggested to alcohol-related oral cavity health disorders [ 8 , 10 ].…”

Section: Introductionmentioning

confidence: 99%

Ethanol binge drinking exposure affects alveolar bone quality and aggravates bone loss in experimentally-induced periodontitis

et al. 2020

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Background Periodontitis is a multifactorial inflammatory disease of tooth supporting tissues caused by oral biofilms, influenced by environmental and genetic factors, among others. Ethanol consumption has been considered a factor that enhances alveolar bone loss, especially in high doses. The present study aims to investigate the changes promoted by ethanol binge drinking per se or associated with ligature-induced periodontal breakdown on alveolar bone loss. Materials and methods Thirty-two Wistar rats were randomly allocated into four groups: control (C), ethanol (3g/kg/day; 3 days On-4 days Off protocol by gavage for 28 days, EtOH), experimental periodontitis (EP) and experimental periodontitis plus ethanol administration (EP+EtOH). On day 14 th , periodontitis was induced by ligatures that were placed around the lower first molars. On day 28 th , the animals were euthanized and mandibles were submitted to stereomicroscopy for exposed root area analysis and micro-computed tomography (micro-CT) for the evaluation of alveolar bone loss and microstructural parameters. Results The results revealed that ligature-induced alveolar bone loss is aggravated by ethanol binge drinking compared to controls (1.06 ± 0.10 vs 0.77 ± 0.04; p<0.0001). In addition, binge drinking per se altered the alveolar bone quality and density demonstrating a reduction in trabecular thickness, trabecular number parameter and bone density percentual. Periodontal disorder plus ethanol binge drinking group also demonstrated reduction of the quality of bone measured by trabecular thickness. Conclusions In conclusion, intense and episodic ethanol intake decreased alveolar bone quality in all microstructural parameters analyzed which may be considered a modifying factor of periodontitis, intensifying the already installed disease.

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Section: Introductionmentioning

confidence: 99%

Ethanol binge drinking exposure affects alveolar bone quality and aggravates bone loss in experimentally-induced periodontitis

et al. 2020

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“…The effects of chronic alcohol consumption on the inflammatory response and expression of osteoclastic markers on apical periodontitis induced in rats were reported in previous studies (Dal‐Fabbro et al . 2019a,b,c). However, none of them assessed the extent of periapical lesions using micro‐CT images.…”

Section: Discussionmentioning

confidence: 99%

“…2017, Dal‐Fabbro et al . 2019a,c). Although 5 weeks seems like a short period of time, the average life span of this species of rats is too short compared to the life expectancy in humans, which justifies this period of exposure to the alcohol as chronic (D’Souza El‐Guindy et al .…”

Section: Discussionmentioning

confidence: 99%

“…2009) and alcohol consumption (Dal‐Fabbro et al . 2019a,c) can increase RANKL expression. The imbalance amongst these components can lead to a major bone resorption in apical periodontitis (Márton & Kiss 2014, Barreiros et al .…”

Section: Introductionmentioning

confidence: 98%

“…Some studies have shown that chronic alcohol consumption enhances the inflammatory response and osteoclastogenesis on induced periapical lesions in rats (Dal‐Fabbro et al . 2019a,b,c), whilst several studies reported a positive association between tobacco smoking and a higher prevalence of apical periodontitis or root canal treatment (Kirkevang & Wenzel 2003, Segura‐Egea et al . 2008, López‐López et al .…”

Section: Introductionmentioning

confidence: 99%

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Effects of alcohol and nicotine consumption on the development of apical periodontitis in rats: a correlative micro‐computed tomographic, histological and immunohistochemical study

et al. 2020

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AimTo evaluate the effects of alcohol and nicotine, when used alone or simultaneously, in the development of apical periodontitis induced in rats, using a correlative analytic approach with micro‐CT, histological and immunohistochemical analysis.MethodologyTwenty‐eight male Wistar rats were arranged into four groups: Control, Nicotine, Alcohol and Alcohol + Nicotine. The alcohol groups were exposed to self‐administration of a 25% alcohol solution, whilst the other groups drunk only filtered water. The nicotine groups received daily intraperitoneal injections of a solution with 0.19 μL of nicotine per mL, whilst the other groups received saline solution. The pulps of the left mandibular first molars were exposed for 28 days to induce periapical lesions. Throughout the experiment, drug administration was maintained, and the animals had their weight and solid and liquid consumption measured. After euthanasia, the mandibles were removed and the area, volume and major diameter of the periapical lesions were measured using micro‐computed tomography images. The samples were submitted to histopathological evaluation and immunohistochemistry for RANKL and PTHrP. Statistical analysis was undertaken with a significance level of 5%. Nonparametric data were analysed using the Kruskal–Wallis test followed by Dunn's test, whilst one‐way anova followed by Tukey's test was performed for parametric data.ResultsThe alcohol groups had lower solid and liquid consumption and gained less weight when compared to the nonalcohol groups (P < 0.05). The Alcohol + Nicotine group had lesions with significantly larger volume and area when compared to the other groups (P < 0.05), whilst the Alcohol or Nicotine groups had significantly larger lesions than the control group (P < 0.05). There was no significant difference in the largest diameter of the lesions amongst groups (P > 0.05). The experimental groups had greater inflammatory response scores than the control group (P < 0.05), and the representative samples had more pronounced immunoreaction against RANKL and PTHrP antibodies.ConclusionsAlcohol and nicotine consumption exacerbated the inflammatory response and the development of periradicular lesions in rats. The association of both substances enhanced their harmful effects.

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Evolution of endodontic medicine: a critical narrative review of the interrelationship between endodontics and systemic pathological conditions

et al. 2021

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Endodontics has gained emphasis in the scientific community in recent years due to the increase in clinical and in animal models studies focused on endodontic medicine, which aims to evaluate the interrelationship between systemic and periapical tissues pathological conditions. These studies have shown that systemic changes can boost the pathogenesis of endodontic infection, favoring its development and progression. A contrary relationship is reported in numerous studies that affirm the potential of endodontic infection to trigger systemic damage and may lead to the worsening of pre-existing pathologies. Recently, the potential of filling materials to develop systemic changes such as neurological alterations had been evaluated, also showing that systemic diseases can negatively influence tissue responses to filling materials after endodontic treatment. Despite advances in endodontic medicine studies, there are still gaps in knowledge on the mechanisms of interactions between apical periodontitis (AP) and systemic diseases and much research to be done. In this sense, this critical narrative literature review aimed to show the evolution of studies in endodontic medicine to help the endodontist to know the role of systemic diseases in the pathogenesis of AP and the possible interference in the repair of periapical tissues after endodontic treatment, as well as to evidence the systemic complications that can be triggered or aggravated in the presence of endodontic infection.

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Effects of different alcohol concentrations on the development of apical periodontitis in rats

Cited by 10 publications

References 53 publications

Ethanol binge drinking exposure affects alveolar bone quality and aggravates bone loss in experimentally-induced periodontitis

Ethanol binge drinking exposure affects alveolar bone quality and aggravates bone loss in experimentally-induced periodontitis

Effects of alcohol and nicotine consumption on the development of apical periodontitis in rats: a correlative micro‐computed tomographic, histological and immunohistochemical study

Evolution of endodontic medicine: a critical narrative review of the interrelationship between endodontics and systemic pathological conditions

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