Effects of Dietary Xylanase and Arabinofuranosidase Combination on the Growth Performance, Lipid Peroxidation, Blood Constituents, and Immune Response of Broilers Fed Low-Energy Diets

Abstract

The present study was conducted to examine that impact of dietary xylanase (Xyl) and arabinofuranosidase (Abf) supplementation on the performance, protein and fat digestibility, the lipid peroxidation, the plasma biochemical traits, and the immune response of broilers. A total of 480, un-sexed, and one-day-old broilers (Ross 308) were randomly divided into three treatments with eight replicates, where chicks in the first treatment were fed basal diets and served as the control, chicks in the second treatment were fed diets formulated with reductions of 90 kcal/kg, and chicks in the third treatment were fed the same formulated diets used in the second group as well as the Xyl and Abf combination (Rovabio® Advance). Feed intake was decreased by the low energy diet, leading to an enhancement in feed efficiency enzyme supplementation in the low energy diet (p < 0.015). Both protein and fat digestibility were improved (p < 0.047) due to enzyme supplementation. Moreover, enzyme supplementation increased muscle total lipids content and decreased muscle thiobarbituric acid retroactive substance content. Furthermore, diets supplemented with Xyl and Abf exhibited an increase in antibody titers against the Newcastle disease virus (p < 0.026). In addition, enzyme supplementation increased gene expression related to growth and gene expression related to fatty acid synthesis. It could be concluded that dietary Xyl and Abf supplementation had beneficial impacts on growth, nutrient digestibility, lipid peroxidation, immune response, and gene expressions related to growth and fatty acid synthesis in broiler chickens fed low-energy diets.

Xylanase and Direct-Fed Microbials (DFM) Potential for Improvement of Live Performance, Energy Digestibility, and Reduction of Environmental Microbial Load of Broilers

“…Less amount of feed is required to produce meat and eggs, and fewer excess nutrients are excreted, thus reducing nutrients available for pathogen proliferation in the hind gut and in the litter ( 16 ). The utilization of low-energy diets to demonstrate the potential of xylanase has been adopted by multiple researchers ( 17 , 18 ). Saleh et al ( 18 ) showed that supplementation of xylanase and arabinofuranosidase to diets formulated with 90 kcal/kg less apparent metabolizable energy (AME) than a standard diet improved growth performance, nutrient digestibility, and immune response of broilers.…”

“…The utilization of low-energy diets to demonstrate the potential of xylanase has been adopted by multiple researchers ( 17 , 18 ). Saleh et al ( 18 ) showed that supplementation of xylanase and arabinofuranosidase to diets formulated with 90 kcal/kg less apparent metabolizable energy (AME) than a standard diet improved growth performance, nutrient digestibility, and immune response of broilers. The ability of xylanase to improve utilization of energy as a result of fiber hydrolysis and release of trapped nutrients could potentially allow for feeding diets with lower energy content thus reducing cost of production.…”

Effect of supplemental serine-protease from Bacillus licheniformis on growth performance and physiological change of broiler chickens

“…In addition, it has been reported that activities of digestive enzymes (lipase, amylase, and trypsin) increase with age (Noy and Sklan 1979;Nitsan et al 1991;Jin et al 1998). Therefore, supplemental exogenous enzymes could optimize the digestibility of CP and consequently growth performance (body weight gain and feed conversion ratio) of broiler chickens (Jiang et al 2008;Angel et al 2011;Kalmendal and Tauson 2012;Yegani and Korver 2013;Saleh et al 2019a).…”

Performance and Carcass Yields of Cobb 500 Broilers Fed Different Dietary Programs and Bioactive Additives

“…In according with these results, Kehlet and Sims (2015) demonstrated that diets with 2% lower energy and supplemented with Bacillus Subtilis have final live weights equal to those of nonsupplemented diets with 2% higher energy. Also, Saleh et al (2019) indicated that feeding low-energy diet significantly decreased body weight compared to that of the control group, whereas diet supplemented with enzymes increased body weight which may be due to the function of enzymes mixture that had a positive effect on the energy utilization (Cowieson and Ravindran, 2008). Different results were reported by Flores et al (2016) who found that the inclusion of xylanase did not have significant impact on body weight or body weight gain throughout the experiment compared to the control group.…”