Effects of dietary white mulberry leaves on hemato-biochemical alterations, immunosuppression and oxidative stress induced by Aeromonas hydrophila in Oreochromis niloticus

Neamat‐Allah, Ahmed N. F.; Mahmoud, Essam A.; Mahsoub, Yasser H.

doi:10.1016/j.fsi.2020.11.028

Cited by 33 publications

(21 citation statements)

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“…Similar pattern of SOD, CAT, GPX activities and MDA level was observed in Nile tilapia fed Morus alba fortified diet post bacteria challenge (Neamat-Allah et al, 2021).ALP, GOT and GPT levels are important markers to assess liver toxicity and damage(Mukherjee et al, 2019). The increased levels of ALP, GOT and GPT in liver homogenate of fish challenged with bacteria compared with those in control unchallenged fish indicated hepatotoxic effect of A. hydrophila Neamat-Allah et al (2021). reported similar hepatotoxic effect of Aeromonas, which elevated ALP, GOT, GPT and LDH levels in infected Nile tilapia, and those were significantly reduced to normal level due to plant extract treatments.…”

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confidence: 63%

“…Haematological parameters indicate the nutritional and health status and hence can be used to assess the physiological condition of the fish (Reverter et al, 2014). A. hydrophila negatively influenced the haematological parameters in treatment groups II and V probably due to haemorrhages in the internal organs and haemolytic activity of the produced haemolysin (Neamat-Allah et al, 2021).…”

Section: Susceptibility and Resistance To Common Antibiotics May Diff...mentioning

confidence: 99%

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Mitigating Aeromonas hydrophila infection in Nile tilapia through dietary Basella alba and Withania somnifera supplementation: A bioassay‐guided fractionation approach

Mukherjee

Ghosal

Marik

et al. 2022

Aquaculture Research

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The present study aimed to identify the antibacterial bioactive compounds in Withania roots and Basella leaves against Aeromonas infection in Nile tilapia. Withania ethanol extract (EW, ZoI 10.72 ± 1.028 mm) and Basella methanol extract (MB, ZoI 10.83 ± 1.058 mm) showed the highest antibacterial activity in disc diffusion assay.In in vivo experiment, Nile tilapia (25.61 ± 0.31 g) were fed basal, EW and MB (both at 0.7 g/kg feed) diets for 15 days either before being intraperitoneally injected with Aeromonas and basal diet feeding for 7 days (prophylactic) or after bacterial injection and basal diet feeding for 7 days (therapeutic). Fish fed EW and MB fortified diets showed significantly (p < 0.05) lower mortality, alkaline phosphatase, glutamic oxaloacetic transaminase, glutamic pyruvate transaminase activities, HSP70, Caspase3, IFN-ɣ, IL-1 β, TNFα level, while significantly (p < 0.05) higher weight gain, protein efficiency ratio, respiratory burst, lysozyme, phagocytotic activity, plasma total protein, total immunoglobulin content, RBC, WBC count, haemoglobin, haematocrit, mean corpuscular haemoglobin concentration, mean corpuscular volume, lymphocytes, neutrophils, monocytes percentages and superoxide dismutase, catalase, glutathione peroxidase activities compared to basal diet-fed fish. EW showed significantly (p < 0.05) better antibacterial efficacy compared to MB. Prophylactic treatment showed better results compared to therapeutic treatment. Bioassay-guided chromatography and GC-MS analysis revealed the presence of hexadecanoic acid, phytol, cis, cis, cis-7,10,13-hexadecatrienal in EW and α-tocopherol, 1-(+)-ascorbic acid 2,6-dihexadecanoate, 1-heptatriacotanol in MB, which was reported to have antibacterial, antioxidative and immunostimulatory potential. Present results indicate prospective use of EW as a prophylactic immunostimulant and effective therapeutic remedy against Aeromonas in Nile tilapia.

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confidence: 63%

Section: Susceptibility and Resistance To Common Antibiotics May Diff...mentioning

confidence: 99%

Mitigating Aeromonas hydrophila infection in Nile tilapia through dietary Basella alba and Withania somnifera supplementation: A bioassay‐guided fractionation approach

Mukherjee

Ghosal

Marik

et al. 2022

Aquaculture Research

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“…The findings in this study suggest a potential approach using phage as prophylactic agent that was effective in protecting Nile tilapia from a MDR A. hydrophila . This approach provided comparable RPS to other promising alternatives to antibiotics, such as probiotic-based or plant-based products (Dawood et al, 2020;Kuebutornye et al, 2020;Naliato et al, 2021;Neamat-Allah et al, 2021). Apart from tilapia, pAh6.2TG has great potential to be applied in catfish aquaculture industry due to the lytic activity of pAh6.2TG against multiple MDRA.…”

Section: Discussionmentioning

confidence: 97%

Characterization and protective effects of lytic bacteriophage pAh6.2TG against a pathogenic multidrug-resistant Aeromonas hydrophila in Nile tilapia ( Oreochromis niloticus )

Dien

Ky²,

Huy³

et al. 2021

Preprint

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Bacteriophage is considered an alternative to antibiotics and environmentally friendly approach to tackle antimicrobial resistance (AMR) in aquaculture. Here, we reported isolation, morphology and genomic characterizations of a newly isolated lytic bacteriophage, designated pAh6.2TG. Host range and stability of pAh6.2TG in different environmental conditions, and protective efficacy against a pathogenic multidrug-resistant (MDR) Aeromonas hydrophila in Nile tilapia were subsequently evaluated. The results showed that pAh6.2TG is a member of the family Myoviridae which has genome size of 51,780 bp, encoding 65 putative open reading frames (ORFs), and is most closely related to Aeromonas phage PVN02 (99.33% nucleotide identity). The pAh6.2TG was highly specific to A. hydrophila and infected 83.3% tested strains of MDR A. hydrophila (10 out of 12) with relative stability at pH 7 9, temperature 0 40 C and salinity 0 40 ppt. In experimental challenge, pAh6.2TG treatments significantly improved survivability of Nile tilapia exposed to a lethal dose of the pathogenic MDR A. hydrophila, with relative percent survival (RPS) of 73.3% and 50% for phage multiplicity of infection (MOI) 1.0 and 0.1, respectively. Significant reduction of bacterial counts in rearing water at 3 h (6.7 ± 0.5 to 18.1 ± 6.98 folds) and in fish liver at 48 h post-treatment (2.7 ± 0.24 to 34.08 ± 26.4 folds) was observed in phage treatment groups while opposite pattern for bacterial counts was observed in untreated control. Interestingly, the surviving fish provoked specific antibody (IgM) against the challenged A. hydrophila. These results might explain the higher survival in phage treatment groups. In summary, the findings suggested that the lytic bacteriophage pAh6.2TG is an effective alternative to antibiotics to control MDR A. hydrophila in tilapia and possibly other freshwater fish.

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“…For many years, medicinal plants have been used to alleviate illnesses in humans (Jarić et al 2015). Due to reported antibiotic resistance cases, medicinal plants in animal production have increased (Abdallah et al 2020a(Abdallah et al , 2020bHashem et al 2020;Mahmoud et al 2020a ;Neamat-Allah et al 2021. One of these plants, garlic, Allium sativum, has antibacterial, antifungal (Leontiev et al 2018), immunomodulation, and anti-inflammatory effects on chickens (Arreola et al 2015;Kyo et al 1999).…”

Section: Introductionmentioning

confidence: 99%

Soothing effect of dietary Allium sativum supplementation on immuno-biochemical alterations, and oxidative stress parameters in E. coli–experimentally infected broiler chickens

Hashem

Neamat‐Allah

Hammza³

et al. 2021

Comp Clin Pathol

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This study evaluated the potential benefit of providing dietary supplements to broiler chickens infected by pathogenic Escherichia coli with Allium sativum (A. sativum). Eighty-one-day-old Hubbard chicks were allocated into four groups (20 for each). The first was a control group (no supplement and not infected). The second received supplements of A. sativum 1 g/ kg feed from day 1 to the end and was not infected. The third group was experimentally infected orally at 7 days old with E. coli O78 10 7 CFU intra-crop and received no supplement. The last group was supplemented with A. sativum and infected with the E. coli O78 as described previously. Chickens infected by E. coli had significantly low lymphocytes and a significant rise in heterophils and monocytes with non-significant changes in total leukocytic count (TLC) compared with the control. The fourth group had non-significant alterations in TLC with significantly low monocytes and heterophils and a significant rise in lymphocytes compared with the third group. The biochemical values of hepatic enzymes, renal, and immunological indicators had severe argumentative alterations related to E. coli infection. Dietary supplementation with A. sativum recovers the chickens' immune indicators in non-infected groups and has an immune-stimulatory effect on E. coli infection. In conclusion, A. sativum mitigates hematological, biochemical, and antioxidant alterations induced by experimentally E. coli infection in broiler chickens.

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Effects of dietary white mulberry leaves on hemato-biochemical alterations, immunosuppression and oxidative stress induced by Aeromonas hydrophila in Oreochromis niloticus

Cited by 33 publications

References 50 publications

Mitigating Aeromonas hydrophila infection in Nile tilapia through dietary Basella alba and Withania somnifera supplementation: A bioassay‐guided fractionation approach

Mitigating Aeromonas hydrophila infection in Nile tilapia through dietary Basella alba and Withania somnifera supplementation: A bioassay‐guided fractionation approach

Characterization and protective effects of lytic bacteriophage pAh6.2TG against a pathogenic multidrug-resistant Aeromonas hydrophila in Nile tilapia ( Oreochromis niloticus )

Soothing effect of dietary Allium sativum supplementation on immuno-biochemical alterations, and oxidative stress parameters in E. coli–experimentally infected broiler chickens

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