Key words:Broiler chickens; Clove; Immunity; Antioxidant; Muscle malondialdehydeOne hundred fifty-one-day old of Cobb broiler chicks were used to investigate the effect of dietary clove bud supplementation on growth performance, immune response and antioxidant status of broiler chickens. Chicks were allotted into five equal groups as the first control that supplemented by the basal diet, the second vitamin E supplemented group fed on the basal diet with vitamin E and selenium supplementation in water, Clove I that fed a basal diet containing 0.5 g clove/kg diet, Clove II that fed a basal diet containing 1.0 g clove/kg diet, and Clove III that fed a basal diet containing 1.5 g clove/kg diet (3 rd , 4 th , and 5 th groups). The GC-MS analysis of an n-hexane extract of clove evidenced the presence of isoeugenol (21.38 %) as a major active ingredient of antioxidant potentials. Clove significantly increased the serum total protein, globulin, IgG, IgM, INF-γ, IL-10, muscle GSH levels, T.SOD, and GST activities. Whereas, the serum total cholesterol, TAG, and muscle MDA levels were significantly decreased. The obtained results stated that BWG, FCR, and PER were improved in Vit. E and Clove I supplemented groups respectively, compared to control and other clove supplemented groups. Although, higher doses of clove had no effect on growth performance parameters with enhancement of immunity and antioxidant activities in broiler chickens that concomitantly, provide a healthy broiler's meat with less MDA that favorable to human consumption.