Effects of dietary tributyrin on intestinal mucosa development, mitochondrial function and AMPK-mTOR pathway in weaned pigs

Wang, Chunchun; Cao, Shuting; Shen, Zhuojun; Hong, Qihua; Feng, Jie; Yan, Peng; Hu, Caihong

doi:10.1186/s40104-019-0394-x

Cited by 24 publications

(28 citation statements)

References 58 publications

(72 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In addition, the genes in M18 are mainly enriched in signal transduction, including Wnt signaling pathway, PI3K-Akt signaling pathway, mTOR signaling pathway, Notch signaling pathway, p53 signaling pathway, and AMPK signaling pathway. These signaling pathways were closely related to cell proliferation, apoptosis, and differentiation [36,[38][39][40][41]. However, the main genes enriched in the KEGG pathway of signal transduction were not affected by solid diet introduction.…”

Section: Molecular Mechanisms Involved In Rumen Wall Morphological Anmentioning

confidence: 97%

Transcriptomic analysis reveals the molecular mechanisms of rumen wall morphological and functional development induced by different solid diet introduction in a lamb model

Sun

Yin

Guo

et al. 2021

J Animal Sci Biotechnol

View full text Add to dashboard Cite

Background This study aimed to elucidate the molecular mechanisms of solid diet introduction initiating the cellular growth and maturation of rumen tissues and characterize the shared and unique biological processes upon different solid diet regimes. Methods Twenty-four Hu lambs were randomly allocated to three groups fed following diets: goat milk powder only (M, n = 8), goat milk powder + alfalfa hay (MH, n = 8), and goat milk powder + concentrate starter (MC, n = 8). At 42 days of age, the lambs were slaughtered. Ruminal fluid sample was collected for analysis of concentration of volatile fatty acid (VFA) and microbial crude protein (MCP). The sample of the rumen wall from the ventral sac was collected for analysis of rumen papilla morphology and transcriptomics. Results Compared with the M group, MH and MC group had a higher concentration of VFA, MCP, rumen weight, and rumen papilla area. The transcriptomic results of rumen wall showed that there were 312 shared differentially expressed genes (DEGs) between in “MH vs. M” and “MC vs. M”, and 232 or 796 unique DEGs observed in “MH vs. M” or “MC vs. M”, respectively. The shared DEGs were most enriched in VFA absorption and metabolism, such as peroxisome proliferator-activated receptor (PPAR) signaling pathway, butanoate metabolism, and synthesis and degradation of ketone bodies. Additionally, a weighted gene co-expression network analysis identified M16 (2,052 genes) and M18 (579 genes) modules were positively correlated with VFA and rumen wall morphology. The M16 module was mainly related to metabolism pathway, while the M18 module was mainly associated with signaling transport. Moreover, hay specifically depressed expression of genes involved in cytokine production, immune response, and immunocyte activation, and concentrate starter mainly altered nutrient transport and metabolism, especially ion transport, amino acid, and fatty acid metabolism. Conclusions The energy production during VFA metabolism may drive the rumen wall development directly. The hay introduction facilitated establishment of immune function, while the concentrate starter enhanced nutrient transport and metabolism, which are important biological processes required for rumen development.

show abstract

Section: Molecular Mechanisms Involved In Rumen Wall Morphological Anmentioning

confidence: 97%

Transcriptomic analysis reveals the molecular mechanisms of rumen wall morphological and functional development induced by different solid diet introduction in a lamb model

Sun

Yin

Guo

et al. 2021

J Animal Sci Biotechnol

View full text Add to dashboard Cite

show abstract

“…Hence, many studies have demonstrated that solely administered TB mediates an anti-inflammatory effect. Mounting evidence suggest that supplementation of TB could induce desirable phenotypes in diet-induced obesity model [ 45 ], in piglets [ 46 , 47 ], and in a murine model of Clostridium difficile infection [ 48 ]. In this study, we identified microbiome-derived genes encoding active TB esterases and sub-cloned them into an E. coli strain with strong intestinal colonization capability [ 31 ].…”

Section: Discussionmentioning

confidence: 99%

An efficient system for intestinal on-site butyrate production using novel microbiome-derived esterases

et al. 2021

View full text Add to dashboard Cite

Short-chain fatty acids, especially butyrate, play beneficial roles in sustaining gastrointestinal health. However, due to limitations associated with direct consumption of butyrate, there has been interest in using prodrugs of butyrate. Tributyrin (TB), a triglyceride composed of three butyrate molecules and a glycerol, is a well-studied precursor of butyrate. We screened a metagenome library consisting of 5760 bacterial artificial chromosome clones, with DNA inserts originating from mouse microbiomes, and identified two clones that efficiently hydrolyse TB into butyrate. Nucleotide sequence analysis indicated that inserts in these two clones are derived from unknown microbes. BLASTp analysis, however, revealed that each insert contains a gene homologous to acetylesterase or esterase genes, from Clostridium spp. and Bacteroides spp., respectively. Predicted structures of these two proteins both contain serine-histidine-aspartate catalytic triad, highly conserved in the family of esterases. Escherichia coli host expressing each of the two candidate genes invariably produced greater amounts of butyrate in the presence of TB. Importantly, administration of TB together with cloned E. coli cells alleviated inflammatory symptoms in a mouse model of acute colitis. Based on these results, we established an efficient on-site and real-time butyrate production system that releases butyrate in a controlled manner inside the intestine.

show abstract

“…Hence, many studies have demonstrated that solely administered TB mediates an anti-in ammatory effect. Mounting evidence suggest that supplementation of TB could induce desirable phenotypes in diet-induced obesity model [45], in piglets [46,47], and in a murine model of Clostridium di cile infection [48]. In this study, we identi ed microbiome-derived genes encoding active TB esterases and sub-cloned them into an E. coli strain with strong intestinal colonization capability [30].…”

Section: Discussionmentioning

confidence: 99%

An Efficient System for Intestinal On-site Butyrate Production Using Novel Microbiome-Derived Esterases

Jung

Yong

Hwang

et al. 2020

Preprint

View full text Add to dashboard Cite

Short-chain fatty acids, especially butyrate, play beneficial roles in sustaining gastrointestinal health. However, due to limitations associated with direct consumption of butyrate, there has been interest in using prodrugs of butyrate. Tributyrin (TB), a triglyceride composed of three butyrate molecules and a glycerol, is a well-studied precursor of butyrate. We screened a metagenome library consisting of 5,760 bacterial artificial chromosome clones, with DNA inserts originating from mouse microbiomes, and identified two clones that efficiently hydrolyse TB into butyrate. Nucleotide sequence analysis indicated that inserts in these two clones are derived from unknown microbes. BLASTp analysis, however, revealed that each insert contains a gene homologous to acetylesterase or esterase genes, from Clostridium spp. and Bacteroides spp., respectively. Predicted structures of these two proteins both contain serine-histidine-aspartate catalytic triad, highly conserved in the family of esterases. Escherichia coli host expressing each of the two candidate genes invariably produced greater amounts of butyrate in the presence of TB. Importantly, administration of TB together with cloned Escherichia coli cells alleviated inflammatory symptoms in a mouse model of acute colitis. Based on these results, we established an efficient on-site and real-time butyrate production system that releases butyrate in a controlled manner inside the intestine.

show abstract

Effects of dietary tributyrin on intestinal mucosa development, mitochondrial function and AMPK-mTOR pathway in weaned pigs

Cited by 24 publications

References 58 publications

Transcriptomic analysis reveals the molecular mechanisms of rumen wall morphological and functional development induced by different solid diet introduction in a lamb model

Transcriptomic analysis reveals the molecular mechanisms of rumen wall morphological and functional development induced by different solid diet introduction in a lamb model

An efficient system for intestinal on-site butyrate production using novel microbiome-derived esterases

An Efficient System for Intestinal On-site Butyrate Production Using Novel Microbiome-Derived Esterases

Contact Info

Product

Resources

About