Effects of dietary threonine supplementation on intestinal barrier function and gut microbiota of laying hens

Abstract

The effects of supplemental dietary threonine (Thr) on laying performance, expression of intestinal mucin 2 (MUC2) and secretory IgA (sIgA), and intestinal microbiota of laying hens fed a low CP diet were investigated. A total of 240 Lohmann Brown laying hens, 28 wk of age, was allocated to 3 dietary treatments, each of which included 5 replicates of 16 hens. Hens were fed a control diet (16% CP), a low CP diet (14% CP), or a low CP diet supplemented with 0.3% L-Thr for 12 weeks. Chemical analyses of the diets for Thr are 0.49, 0.45, and 0.69%, respectively. Lowering dietary CP impaired egg production and egg mass of laying hens. Dietary Thr supplementation to the low CP diet increased (P < 0.05) egg production and egg mass. In addition, ileal sIgA contents and MUC2 and sIgA mRNA expression were increased (P < 0.05) by dietary Thr addition. Dietary CP reduction reduced (P < 0.05) intestinal bacterial diversity, whereas dietary Thr supplementation to the low CP diet recovered the bacteria diversity and significantly increased the abundance of potential beneficial bacteria. In conclusion, dietary Thr supplementation to a low CP diet could affect intestinal health and hence productivity via regulating intestinal mucin and sIgA expression, and microbial population of laying hens.

Growth performance, carcass characteristics, meat and egg quality, and intestinal microbiota in Beijing-you chicken on diets with inclusion of fresh chicory forage

“…During the processes of nutrition, metabolism, physiology and immunity, the intestinal microbiota can promote digestion and absorption of nutrients, stimulate the immune response of the host and enhance resistance to infection ). In addition to animal age, hygiene and environmental factors, diet is a major factor that can influence the microbial population in the intestinal tract (Dong et al 2017). Previous studies suggested that dietary supplementation of forage had an impact on poultry performance by modifying the gut microbial structure to a bacterial community that is more conducive to host growth (Jiang et al 2014;Saeed et al 2017).…”

Maternal Dietary Linoleic Acid Altered Intestinal Barrier Function in Domestic Pigeons (Columba Livia)

“…increased the diversity of intestinal microbiota in squabs. It is widely believed that the higher the diversity, the better the intestine health, because the diversity is one of the key determinants of colonization resistance against invading pathogens [51]. The result indicated that squabs had greater resistance in LA1% and LA2%.…”

Age dynamics of growth and development of chichens depending on protein metabolism

“…На даному етапі деталізація технології вирощування здійснюється на високому рівні з врахуванням факторів, яким раніше вчені і практики не приділяли уваги. Наприклад, мікроструктурні зміни в кишечнику, зокрема, в слизових оболонках від яких залежить асиміляція нутрієнтів, стан і процеси які відбуваються у кишечнику визначають здоров'я птиці та ефективність використання поживних і біолого активних речовинах, що в свою чергу, пов'язано з інтенсивністю росту і розвитку, конверсією корму та іншими важливими господарськими показниками в птахівництві (Grozina, 2014;Filho et al, 2015;Dong et al, 2017). Відомо, що у птахівництві біля 70% собівартості продукції припадає на вартість кормів.…”