Effects of dietary T-2 toxin on gut health and gut microbiota composition of the juvenile Chinese mitten crab (Eriocheir sinensis)

Wang, Xiaodan; Huang, Yuxing; Bu, Xianyong; Xiao, Shusheng; Qin, Chuanjie; Qiao, Fang; Qin, Jian G.; Chen, Liqiao

doi:10.1016/j.fsi.2020.08.019

Cited by 25 publications

(10 citation statements)

References 67 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The majority of Tenericutes have been reported as either commensal or obligate parasites of domestic animals, plants, insects, and humans [ 95 ]. According to a recent metagenomic analysis, over 100 uncultured Tenericutes were discovered in the human gastrointestinal tract [ 96 ], but it still remains unclear whether these novel lineages are contributing to the maintenance of gut homeostasis and the overall microbiome function [ 97 ]. Regarding the presence of Tenericutes in T1D, lower relative abundances have been previously reported in humans with T1D and in non-obese T1D mice compared to non-diabetic controls [ 98 , 99 ].…”

Section: Resultsmentioning

confidence: 99%

“…Candidatus bacilloplasma belongs taxonomically to the Tenericutes phylum, and the observed increase in its levels was in accordance with the correspondingly increased levels of Tenericutes. Notably, Candidatus bacilloplasma has been reported as an opportunistic pathogen associated with gastrointestinal diseases in aquatic organisms [ 97 , 103 ], but such findings have not been confirmed in either humans or rodents. In addition, increased relative abundances of Eubacterium and Coprococcus were recorded in the feces of diabetic rats compared to the control group, but only in the case of Coprococcus was this increase statistically significant ( p = 0.026).…”

Section: Resultsmentioning

confidence: 99%

See 1 more Smart Citation

Corinthian Currants Supplementation Restores Serum Polar Phenolic Compounds, Reduces IL-1beta, and Exerts Beneficial Effects on Gut Microbiota in the Streptozotocin-Induced Type-1 Diabetic Rat

et al. 2023

View full text Add to dashboard Cite

The present study aimed at investigating the possible benefits of a dietary intervention with Corinthian currants, a rich source of phenolic compounds, on type 1 diabetes (T1D) using the animal model of the streptozotocin-(STZ)-induced diabetic rat. Male Wistar rats were randomly assigned into four groups: control animals, which received a control diet (CD) or a diet supplemented with 10% w/w Corinthian currants (CCD), and diabetic animals, which received a control diet (DCD) or a currant diet (DCCD) for 4 weeks. Plasma biochemical parameters, insulin, polar phenolic compounds, and inflammatory factors were determined. Microbiota populations in tissue and intestinal fluid of the caecum, as well as fecal microbiota populations and short-chain fatty acids (SCFAs), were measured. Fecal microbiota was further analyzed by 16S rRNA sequencing. The results of the study showed that a Corinthian currant-supplemented diet restored serum polar phenolic compounds and decreased interleukin-1b (IL-1b) (p < 0.05) both in control and diabetic animals. Increased caecal lactobacilli counts (p < 0.05) and maintenance of enterococci levels within normal range were observed in the intestinal fluid of the DCCD group (p < 0.05 compared to DCD). Higher acetic acid levels were detected in the feces of diabetic rats that received the currant diet compared to the animals that received the control diet (p < 0.05). Corinthian currant could serve as a beneficial dietary component in the condition of T1D based on the results coming from the animal model of the STZ-induced T1D rat.

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Corinthian Currants Supplementation Restores Serum Polar Phenolic Compounds, Reduces IL-1beta, and Exerts Beneficial Effects on Gut Microbiota in the Streptozotocin-Induced Type-1 Diabetic Rat

et al. 2023

View full text Add to dashboard Cite

show abstract

“…It has been proven that in flies and mice, PIWI/piRNA can inhibit transposable elements (Saito et al 2006, Brennecke et al 2007, Aravin et al 2008, Kuramochi-Miyagawa et al 2008. In addition, in some crustaceans, transposable elements can be inhibited by the argonaute proteins (Leebonoi et al 2015, Sukthaworn et al 2019, 2020. In addition to the silencing of transposable elements, piRNAs also have a role in regulating the spermatogenic gene mRNA expression through siRNA-like bp way.…”

Section: Discussionmentioning

confidence: 99%

“…The roles of PIWI proteins in vertebrates and Drosophila are better understood, but in other invertebrates, their roles remain unclear, especially in crustaceans. Little is known about Piwi proteins in crustaceans except for Portunus trituberculatus, Penaeus monodon, and Scylla paramamosain (Xiang et al 2014, Sukthaworn et al 2019, 2020, Waiho et al 2020. In P. monodon, Piwi proteins have a role in controlling the expression of transposable elements, and knockdown of Piwi proteins decreases the number of sperm and the weight of the testis (Sukthaworn et al 2019).…”

Section: Introductionmentioning

confidence: 99%

PIWIs maintain testis apoptosis to remove abnormal germ cells in Eriocheir sinensis

Wei

Tong

et al. 2021

Reproduction

View full text Add to dashboard Cite

PIWI proteins play important roles in germline development in the mammals. However, the functions of PIWIs in crustaceans remain unknown. In the present study, we identified three Piwis from the testis of Eriocheir sinensis (E. sinensis). Three Piwi genes encoded proteins with typical features of PIWI subfamilies and were highly expressed in the testis. Three PIWIs could be detected in the cytoplasm of spermatocytes and spermatids, while in spermatozoa, we could only detect PIWI1 and PIWI3 in the nucleus. The knockdown of PIWIs by dsRNA significantly affected the formation of the nuclei in spermatozoa, which resulted in deviant and irregular nuclei. PIWI defects significantly inhibited the apoptosis of abnormal germ cells through the caspase-dependent apoptosis pathway and p53 pathway. Knockdown of PIWIs inhibited the expression of caspase3, 7, 8, and p53 without affecting Bcl2 (B-cell lymphoma gene 2), Bax (B-cell lymphoma-2-associated X) and BaxI (B-cell lymphoma-2-associated X inhibitor), which further significantly increased abnormal spermatozoa in the knockdown-group crabs. These results show a new role of PIWI proteins in crustaceans that is different from that in mammals. In summary, PIWIs play roles in the formation of the germline nucleus and can maintain apoptosis in abnormal germ cells to remove abnormal germ cells in E. sinensis.

show abstract

“…(15) продемонстрировали, что кишечная микробиота воздействует на экспрессию генов GSTs, GPX2, EPHXs и NNAT 1, связанных с детоксикацией ксенобиотиков, в тканях толстого кишечника и печени хозяина. Исследования, касающиеся изучения состава микробиома животных и птиц под влиянием микотоксинов крайне ограничены (16,17). Анализ изменений прогнозируемого функционального потенциала микробиома животных, птиц и человека с применением биоинформатических программных комплексов, таких как PICRUSt2 и ему подобных, ранее не проводился.…”

unclassified

COMPOSITION AND METABOLIC POTENTIAL OF THE INTESTINAL MICROBIOME OF Gallus gallus L. BROILERS UNDER EXPERIMENTAL T-2 TOXICOSIS AS INFLUENCED BY FEED ADDITIVES

Yildirim¹

2022

S-h. biol.

View full text Add to dashboard Cite

Микотоксины негативно влияют на состав и функции кишечной микробиоты у сельскохозяйственной птицы и, как следствие, на ее здоровье. Одной из стратегий восстановления микробиома и борьбы с микотоксикозами стало введение добавок в загрязненные корма. В настоящей работе впервые показано, что кормовая добавка Заслон 2+ эффективно улучшает структуру и метаболический потенциал кишечного микробиома у цыплят-бройлеров при экспериментальном Т-2 микотоксикозе. Нашей целью было выявление изменений микробиоты химуса и ее функциональная аннотация после 14-суточного воздействия Т-2 токсина, искусственно внесенного с кормами, и под влиянием кормовой добавки Заслон 2+, применяемой отдельно и в сочетании с протеолитическим препаратом Axtra Pro на фоне экспериментального микотоксикоза. Опыты проводили в 2021 году в виварии Всероссийского научно-исследовательского и технологического института птицеводства на цыплятах-бройлерах кросса Смена 8 (с 33-до 47-суточного возраста). Корм контаминировали Т-2 токсином механическим способом. Птицу разделили на четыре группы по 5 гол. в каждой: I группа (контроль) получала основной рацион (ОР) без введения Т-2 токсина, II группа -ОР с добавлением Т-2 токсина (200 мкг/кг, 2-кратное превышение ПДК), III -ОР с добавлением Т-2 токсина (200 мкг/кг) и кормовую добавку Заслон 2+, состоящую из сорбирующего материала диатомита, двух культур бактерий Bacillus spp., смеси натуральных эфирных масел эвкалипта, чабреца, чеснока и лимона (ООО «БИОТРОФ», Россия) (1 г/кг корма), IV -ОР с добавлением Т-2 токсина (200 мкг/кг), кормовую добавку Заслон 2+ (1 г/кг корма) и ферментный препарат с протеолитической активностью Axtra Pro («DuPont de Nemours, Inc.», США) (100 мг/кг корма). Потребление корма бройлерами составляло в среднем 150 г/сут, то есть птицы опытных групп ежесуточно получали Т-2 токсин с кормом в количестве 30 мкг. В конце эксперимента проводили отбор проб химуса слепых отростков кишечника от трех птиц из каждой группы. Тотальную ДНК из исследуемых образцов выделяли с использованием набора «Genomic DNA Purification Kit» («Fermentas, Inc.», Литва). Бактериальное сообщество слепой кишки оценивали методом NGS-секвенирования на платформе MiSeq («Illumina, Inc.», США) с применением праймеров для V3-V4 региона гена 16S рРНК. Биоинформатический анализ данных выполняли с помощью программного обеспечения QIIME2 ver. 2020.8 (https://docs.qiime2.org/2020.8/). Реконструкцию и прогнозирование функционального содержания метагенома, семейств генов, ферментов осуществляли при помощи программного комплекса PICRUSt2 (v.2.3.0) (https://github.com/picrust/picrust2). Для анализа метаболических путей и ферментов пользовались базой данных MetaCyc (https://metacyc.org/). При проведении NGS-секвенирования микробиома было показано, что в кишечнике у бройлеров из II группы происходили сдвиги в биоразнообразии и составе микробиома уже на уровне филумов: обилие бактерий суперфилума Actinobacteriota и филума Proteobacteria возрастало соответственно в 1,8 и 3,5 раза (р  0,05), суперфилума Desulfobacterota -напротив, снижалось в 2...

show abstract

Effects of dietary T-2 toxin on gut health and gut microbiota composition of the juvenile Chinese mitten crab (Eriocheir sinensis)

Cited by 25 publications

References 67 publications

Corinthian Currants Supplementation Restores Serum Polar Phenolic Compounds, Reduces IL-1beta, and Exerts Beneficial Effects on Gut Microbiota in the Streptozotocin-Induced Type-1 Diabetic Rat

Corinthian Currants Supplementation Restores Serum Polar Phenolic Compounds, Reduces IL-1beta, and Exerts Beneficial Effects on Gut Microbiota in the Streptozotocin-Induced Type-1 Diabetic Rat

PIWIs maintain testis apoptosis to remove abnormal germ cells in Eriocheir sinensis

COMPOSITION AND METABOLIC POTENTIAL OF THE INTESTINAL MICROBIOME OF Gallus gallus L. BROILERS UNDER EXPERIMENTAL T-2 TOXICOSIS AS INFLUENCED BY FEED ADDITIVES

Contact Info

Product

Resources

About