Effects of dietary supplementation with peony pollen on growth, intestinal function, fillet quality and fatty acids profiles of common carp

Abstract

A 6‐week feeding trial was conducted to investigate the effects of dietary supplementation with peony pollen on growth performance, intestinal function, fillet quality and fatty acids profiles of common carp. The results showed that supplementation of peony pollen significantly improved the growth performance. The textures of muscle in terms of hardness, springiness, cohesiveness, gumminess, chewiness and resilience were higher in peony pollen groups than control group. Dietary supplementation with peony pollen significantly improved the fold height, enterocyte height, microvillus height, intestinal wall thickness and intestinal crypt height of mid‐intestine from common carp in contrast to basal diet. Moreover, peony pollen could significantly increase digestive capacity observed in terms of an increase in lipase, amylase and trypsase activities. Peony pollen supplementation enhanced the antioxidant capacity via an increase in catalase, and total antioxidant capacity, accompanied by a significant reduction in malondialdehyde. Dietary supplementation with peony pollen may regulate lipid metabolism and fatty acids composition of common carp. Overall, the peony pollen could improve the fish health status by elevating immune responses and antioxidant capacity of intestine and system immunity. And the optimum concentration was recommend is 30 g peony pollen per kilogram feed. Further studies on the peony pollen practical approaches during culturing periods in the aquaculture industry are needed.

Effects of Dietary Supplementation with Honeybee Pollen and Its Supercritical Fluid Extract on Immune Response and Fillet’s Quality of Farmed Gilthead Seabream (Sparus aurata)

“…This trend was also associated with ABTS values, as P10, E1 and P5 showed lower decreasing values than CTR and E0.5. Herein, the present result did not support previous evidence regarding the beneficial effects on the overall antioxidant capacity and malondialdehyde content of the intestine of common carp fed with 30 g/kg of peony pollen [23]. Indeed, the antioxidant capacity expressed in E1, P10 and P5 was not sufficient to overdue the lipid oxidation caused by the storage.…”

“…Although immunostimulatory properties have been proposed for bee pollen in several species, the effect of dietary supplementation with HBP_SFE on immune response and fillet quality has not been previously described. In a recent study, Ren et al [23] observed that peony pollen diet supplement significantly promoted the growth performance, improved intestinal health and digestive enzymes activity of common carp (Cyprinus carpio). Furthermore, they suggested that the use of peony pollen could improve the fish health status by increasing highly unsaturated fatty acids (HUFA) content, improving intestinal health, elevating immune responses and antioxidant capacity of common carp organs [23].…”

“…In a recent study, Ren et al [23] observed that peony pollen diet supplement significantly promoted the growth performance, improved intestinal health and digestive enzymes activity of common carp (Cyprinus carpio). Furthermore, they suggested that the use of peony pollen could improve the fish health status by increasing highly unsaturated fatty acids (HUFA) content, improving intestinal health, elevating immune responses and antioxidant capacity of common carp organs [23]. In the present study, the inclusion of bee pollen in the S. aurata diet affected the levels of some markers related to the immunity of fish and some quality parameters of fillets.…”

“…However, the content of C18:3n-3, the fatty acid precursor of EPA and DHA, found higher in the E1 group compared to the other diets, could deserve further investigations to better understand the interaction between HBP and the expression of genes involved in lipid metabolism. Indeed, peony pollen administrated for 6 weeks to common carp might regulate fish lipid metabolism and fatty acids profile [23]. Concerning lipid stability, the frozen storage slightly increased the relative abundance of myristic (C14:0), palmitic (C16:0) and palmitoleic (C16:1n-7) acids.…”

“…It has been observed that zebrafish fed with a bee pollen-added diet showed changes in the expression of immunity-related genes with improvement in the immune response [18], and characteristics of fillet [19]. Other studies tested bee pollen in animal diets also demonstrating biological effects and nutritional functions positive for growth performance [19][20][21][22][23], immunity [8,[22][23][24], intestinal function [23,25,26], reproductive traits [20,22] and/or meat fish color and quality [19,23]. However, negative effects of dietary HBP were highlighted in meagre (Argyrosomus regius) [15], probably because pollen can be a source of various anti-nutritional compounds and indigestible fractions.…”