Effects of Dietary Supplementation with Cysteamine on Growth Hormone Receptor and Insulin-Like Growth Factor System in Finishing Pigs

Abstract

The present study was conducted to test the hypothesis that chronic cysteamine (CS) supplementation may affect serum insulin-like growth factor (IGF)-I concentrations and growth hormone (GH) receptor (GHR), IGF-I, IGF-I receptor (IGF-IR), IGF binding protein (IGFBP)-3, and insulin receptor (IR) mRNA levels in different tissues of finishing pigs. A total of 24 finishing pigs (60.05 +/- 1.24 kg; 12 gilts and 12 barrows) were assigned randomly to one of the three dietary groups, with four pens/group (per pen: one gilt, one barrow). The pigs were fed a basal diet containing 0 (control), 70, or 140 mg/kg cysteamine feed additive (containing 28% cysteamine hydrochloride) for 47 days. The results indicated that CS supplementation (70 mg/kg) increased the average daily gain (ADG) and serum IGF-I level, upregulated mRNA levels of GHR and IGF-I (liver, stomach, muscle), IGF-IR (stomach, duodenum, muscle), and IGFBP-3 (liver) but downregulated IGFBP-3 (stomach, duodenum, muscle). CS supplementation (70 mg/kg) did not affect mRNA levels of GHR and IGF-I (duodenum), IGF-IR (liver), and IR (liver, stomach, duodenum, muscle). CS supplementation (140 mg/kg) downregulated GHR (duodenum), IGF-I, and IGF-IR mRNA (liver, stomach, duodenum, muscle) but upregulated IGFBP-3 and IR mRNA (liver, stomach, duodenum, muscle) and did not affect ADG and serum IGF-I concentration. Collectively, the results suggest that dietary CS supplementation modulates the growth rate, serum IGF-I concentrations, and the gene expression of GHR, IGF-I, IGF-IR, IGFBP-3, and IR in a dose-dependent manner. CS supplementation has tissue-specific regulation of GHR, IGF-I, IGF-IR, and IGFBP-3 mRNA levels. Moreover, the results also imply the possible physiologic role of the GH-IGF axis in mediating the dietary CS supplementation-supported growth of finishing pigs.

Chromium Improves Protein Deposition Through Regulating the mRNA Levels of IGF-1, IGF-1R, and Ub in Rat Skeletal Muscle Cells

“…IGF-1 increased amino acid transport [18] and protein synthesis [19] in the cultured cells in vitro. IGF-I can also stimulate amino acid uptake and protein synthesis in muscles and can greatly reduce the rate of protein breakdown within muscle fibers [20]. In the present study, insulin increased the mRNA levels of IGF-1, and this effect could be enhanced by the addition of different forms of chromium: CrSP is the highest, followed by CrPic, then CrCl 3 .…”

Transcriptome profiling of in vitro-matured oocytes from a korean native cow (hanwoo) after cysteamine supplementation

“…28 Our data highlighted that LOC781250 upregulation might be due to increased GSH content in oocytes, which acts as a reservoir to protect zygotes and promotes embryonic development. Our data support the results of the study by Guangmang et al 29 who established that IGF-I mRNA levels were upregulated after dietary supplementation with cysteamine on growth hormone receptor and insulin-like growth factor in finishing pigs. 29 It also increases the proportion of preimplantation embryos developing into blastocysts, alters blastocyst gene expression, and improves embryo resistance to various stresses.…”

Effects of dietary coated cysteamine hydrochloride on pork color in finishing pigs

“…In many studies, the positive correlation between cysteamine and growth performance and FE has been found in fishes, rats, pigs, and broilers . In our study, no significant difference was found in feed conversion ratio, thus corroborating the study by Liu et al ., who also found no significant difference in FE between treatments with 0.070 g kg −1 cysteamine and control in finishing pigs. These results indicate that increasing the feed conversion ratio by at least 8% through dietary CC might improve economic returns in swine industry.…”