Effects of Dietary Supplementation with <i>Aspergillus Awamori </i>on Growth Performance and Antioxidative Status of Broiler Chickens Exposed to High Ambient Temperature

Abstract

This study was conducted to evaluate the effects of dietary supplementation with Aspergillus awamori on the growth performance and antioxidative status in male broiler chickens exposed to high ambient temperatures. Twentyfour 15-d-old male broiler chickens were randomly divided into 2 dietary groups fed a corn-soybean meal-based diet supplemented without or with 0.05% of Asp. awamori. Six birds of each dietary group were kept under a thermoneutral condition (22℃), and 6 birds were exposed to heat stress (35℃, 9 h/d) for 15 d. Dietary Asp. awamori decreased feed intake and improved the feed conversion ratio in chickens kept at 22℃. Body weight gain and feed intake were decreased in chickens exposed to heat stress. There was no beneficial effect of dietary Asp. awamori on growth performance under the heat stress condition. However, the malondialdehyde content in skeletal muscle, as an index of lipid peroxidation, was decreased by dietary Asp. awamori in chickens kept at 22℃. Although the malondialdehyde content increased under the heat stress condition, dietary Asp. awamori alleviated the increased malondialdehyde. In addition, the α-tocopherol content in skeletal muscle was negatively affected by the exposure to heat stress, and dietary Asp. awamori recovered the reduction in the α-tocopherol content. Moreover, mRNA expression of glutathione peroxidase in liver was increased by dietary Asp. awamori under both of the thermal conditions. In conclusion, this study shows that dietary Asp. awamori improves the diminished antioxidative status under the heat stress condition partially because of its effect on the expression of hepatic antioxidant enzymes in broilers.

Effects of astaxanthin‐rich dried cell powder from Paracoccus carotinifaciens on carotenoid composition and lipid peroxidation in skeletal muscle of broiler chickens under thermo‐neutral or realistic high temperature conditions

“…These chicks were housed individually in wire‐bottomed aluminum cages (50 × 40 × 60 cm) and fed a basal diet (see Table for diet composition) for 3 days until beginning of the main experimental period. Heat exposure experiment was designed according to our previous studies (El‐Deep, Ijiri, Ebeid, & Ohtsuka, ; El‐Deep, Ijiri, Eid, Yamanaka, & Ohtsuka, ). Panaferd‐P supplementation (0.15%) was determined to adjust to the Astaxanthin concentration (30 ppm) of the previous study (Akiba, Sato, Takahashi, Matsushita, et al., ).…”

Beneficial effects of Aspergillus awamori in broiler nutrition

“…These results indicate that A. awamori produces antioxidative substances. Indeed, an increase in TBARS levels in the breast muscle of broiler chickens induced by heat stress was negated by feeding a diet containing A. awamori (El-Deep et al, 2014). In addition, feeding diets containing A. awamori increased the mRNA expressions of antioxidant enzymes (i.e., glutathione peroxidase, catalase and superoxide dismutase).…”

Effect of Asperagillus awamori on Alloxan-induced Mouse Hyperglycemia

“…The result indicated that hyperglycemia induced by alloxan administration was ameliorated by feeding of only A. awamori. Several researchers reported that dietary intake of A. awamori improved an intestinal environment and induced increase of α-tocopherol (vitamin E) level in their blood and muscles to affect the lipid and protein metabolism (El-Deep et al, 2014;Saleh et al, 2013Saleh et al, , 2014. As the intake of vitamin E improved mouse hyperglycemia caused by alloxan and vitamin E improved insulin release from pancreas damaged by alloxan (Kamimura et al, 2013;Takemoto, Doi, & Masuoka, 2016), we deduced that A. awamori is an effective probiotic and increases vitamin E to ameliorate hyperglycemia induced by oxidative stress.…”