Effects of dietary supplementation of linseed oil (Omega-3) on quality parameters of Nili Ravi bull spermatozoa

Ahmad, Harith; Ali, Sultan; Abbas, Amjad; Abbas, Rao Zahid; Zubair, Muhammad; Raza, Muhammad Asif; Bano, Naheed; Shafi, M.; Badar, Syed Nadeem; Hussain, Kashif; Rizwan, Muhammad; Ahmad, Tauseef

doi:10.1016/j.livsci.2019.04.007

Cited by 6 publications

(5 citation statements)

References 11 publications

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“…were both not supplemented with flaxseed oil and it is known that low DHA in the sperm cell has been associated with the infertility (Moallem et al 2015 ). Furthermore, dietary supplementation of omega n-3 sources is proven to increase sperm and subsequent fertilization rate motility (Ahmad et al 2019 ) thus the great interest in dietary inclusion of dietary omega n-3 precursors on domestic livestock diets (Díaz et al 2017 ). Despite other numerous factors affecting the efficient of in vitro embryo production, semen quality (fresh or frozen) and the selection of semen has been major factors (Falchi et al 2018 ).…”

Section: Discussionmentioning

confidence: 99%

Dietary supplementing South African indigenous rams with flaxseed oil and ascorbic acid improves cryopreserved semen quality and in vitro fertility

Ngcobo,

Nedambale,

Nephawe

et al. 2024

Trop Anim Health Prod

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The purpose of this study was to evaluate how ascorbic acid with dietary flaxseed oil affects the quality and fertility of cryopreserved ram sperm in South African indigenous rams. Treatment diets were supplemented 60 days before semen collection to afford proper spermatogenesis, adaptation to the feed formulated and fed throughout the study. Semen was collected with the use of artificial vagina following dietary supplementation with five treatment diets (neg. cont. – negative control, pos. cont. – positive control, FLO – 5% Flaxseed oil, ASA – 4% Ascorbic acid, and FLO + ASA). Semen was then extended using tris-based extender and cryopreserved using the programmable freezer (CBS Freezer 2100 series, Laboratory consumables & chemical suppliers, America). Ovaries were collected from a neighbouring slaughter house and conveyed to the lab in 0.9% saline at 37 °C. Data (sperm parameters and in vitro fertility) was then exposed to the GLM (General Linear Model) in Minitab 17. Pearson’s correlation coefficient was utilized to investigate the relationship between cryopreserved sperm quality and in vitro fertility. The student Least Significant Difference Test was used to separate the treatment means, and differences were accepted when the p-value was less than 0.05. The FLO + ASA group had higher (p < 0.05) progressive (36.33 ± 1.87), total (88.24 ± 2.24), rapid motility (27.52 ± 1.74), intact plasma membrane (75.67 ± 2.08), total fertilization (65.98 ± 7.39), and total cleavage (66.19 ± 6.50) when compared to other treatment groups. Total fertilization rate had a medium significant (p < 0.001) medium correlation with the progressive motility (r2 = 0.435), total motility (r2 = 0.447) and rapid motility (r2 = 0.409). In conclusion, dietary flaxseed and ascorbic acid (FLO + ASA) improves cryopreserved semen quality, in vitro fertilization rate, and the total cleavage rate. Noteworthy, the progressive, total and rapid motility play a crucial in the in vitro fertilization rate.

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Section: Discussionmentioning

confidence: 99%

Dietary supplementing South African indigenous rams with flaxseed oil and ascorbic acid improves cryopreserved semen quality and in vitro fertility

Ngcobo,

Nedambale,

Nephawe

et al. 2024

Trop Anim Health Prod

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“…It regulates the permeability of bacterial membrane is controlled by their CRISPR Cas system (Chen and Guan, 2016). It makes bacteria resistant to membrane targeting Antibiotics (Ahmad et al 2019). Regulation of membrane permeability helps bacteria to resist recognition by multiple host receptors to enhance pathogenicity (Chen and Guan, 2016).…”

Section: The Crispr-cas System Promotes Antimicrobial Resistance In Campylobacter Jejunimentioning

confidence: 99%

CRIPSR Case System: Biological Role in Bacterial Virulence, Genome Editing and in Antimicrobial Resistance

Rizwan¹,

Arshad²,

Kashif³

et al. 2021

PUJZ

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The aim of the present review was to study about the CRISPR Cas system and there role in genome editing, bacterial virulence and antibiotics resistance. CRISPR Cas system is an integral part of prokaryotic (Bacteria and Archaea) immune system that provides protection against viral infection. When bacteria recognize viral DNA inside it, bacteria incorporate small fragment of viral DNA into its genome at specific site termed as CRISPR locus. Insertion of viral DNA at CRISPR locus allows to remember, diagnose and clear the viral infection by the mechanism of sequence specific Adaptive Immunity. CRISPR Cas system is sustainable to combate with the mutation developed in viral genome that help viruses to escape from bacterial CRISPR Cas based immune system. CRISPR Cas system is a molecular mechanism of prokaryotic microorganism. It acts as bacterial natural adaptive immune system against phages, plasmids and foreign genomic elements. Mostly prokaryotes uses their CRIPR Cas system to enhance the integrity of their cell membrane that inhibit the permeability of antimicrobials from host body into the bacterial cells. CRISPR Cas system also help bacteria to evade from the host immune system by suppressing the activity of their immune receptors e.g. TLR. CRISPR Cas system also help bacteria to attach with the host body and replicate with in host body. It can develop antibiotic resistance in pathogenic bacteria, enhance their pathogenicity and can survival in host body.Novelty Statement | CRISPR Cas system is an integral part of prokaryotic immune system that provides protection against viral infection and involved in genome editing, bacterial virulence and antibiotics resistance.

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“…The spermatozoa membrane composition is critical for capacitation [20], acrosome reaction [21], and spermatozoa-oocyte fusion through fertilization [22]. In mammalians the spermatozoa membrane is rich in PUFA, mainly docosahexaenoic acid and docosapentaenoic acid [22].…”

Section: Introductionmentioning

confidence: 99%

Carryover effects of feeding bulls with an omega-3-enriched-diet—From spermatozoa to developed embryos

et al. 2022

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The impact of omega-3 nutritional manipulation on semen cryosurvival and quality post thawing is controversial. Our aim was to examine how feeding bulls with omega-3 supplementation from different sources affects the spermatozoa quality parameters. Fifteen Israeli Holstein bulls were fed for 13 weeks with a standard ration top-dressed with encapsulated-fat supplementation: fish or flaxseed oil or saturated fatty acids (control). Ejaculates were collected before, during, and after the feeding trial. Frozen–thawed samples were evaluated by a flow cytometer for spermatozoa viability, mitochondrial membrane potential, the level of reactive oxygen species (ROS), acrosome membrane integrity, DNA fragmentation, phosphatidylserine translocation, and membrane fluidity. Both fish and flaxseed oil treatment resulted in lower ROS levels vs. control groups, during and after the feeding trial. Fewer spermatozoa with damaged acrosomes were observed in the fish oil group after the feeding trial. The spermatozoa membrane fluidity was altered in both the fish and flaxseed oil groups throughout the feeding trial, but only in the flaxseed oil group after the feeding trial. The proportion of spermatozoa with fragmented DNA was lower in the flaxseed oil group after the feeding trial. The spermatozoa fertilization competence did not differ between groups however, blastocyst formation rate was higher in the fish and flaxseed oil groups relative to the control. This was associated with differential gene expression in the blastocysts. Overall, the omega-3-enriched food improved the spermatozoa characteristics; this was further expressed in the developing blastocysts, suggesting a carryover effect from the spermatozoa to the embryos.

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Effects of dietary supplementation of linseed oil (Omega-3) on quality parameters of Nili Ravi bull spermatozoa

Cited by 6 publications

References 11 publications

Dietary supplementing South African indigenous rams with flaxseed oil and ascorbic acid improves cryopreserved semen quality and in vitro fertility

Dietary supplementing South African indigenous rams with flaxseed oil and ascorbic acid improves cryopreserved semen quality and in vitro fertility

CRIPSR Case System: Biological Role in Bacterial Virulence, Genome Editing and in Antimicrobial Resistance

Carryover effects of feeding bulls with an omega-3-enriched-diet—From spermatozoa to developed embryos

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