The objective was to examine the effects of supplementing different levels of zeolite in the diet of laying hens during the laying phase, at 54 weeks of age, on production performance, egg quality, ammonia volatilization, excreta pH, blood parameters, weight, and organ morphometry. The treatments consisted of a control diet free of zeolite and five diets including increasing levels of zeolite (0.7, 1.4, 2.1, 2.8, and 3.5%). A completely randomized design was adopted with eight replicates of five birds per plot, totaling 240 laying hens, for four 28-day periods. Results were subjected to ANOVA, Dunnett's test, and regression analysis at 5% probability. The zeolite levels had no significant effect on production performance, egg quality, and blood parameters. The zeolite levels have a significant effect on ammonia volatilization, excreta pH, liver weight, and organ morphometry specifically on the intestinal length. Ammonia and pH reached a linear plateau. Ammoniacal nitrogen levels in the excreta differed from that observed in the control group at all zeolite levels, whereas the pH was lowest at the zeolite inclusion levels of 0.7 and 1.4%. Ammonia volatilization and the pH value of the excreta decreased by 46.9% and 4.6%, when the laying hens consumed diets with 0.93% and 1.19% zeolite, respectively. There was an effect on the relative weight of the organs, with higher liver yield and intestinal length provided by zeolite inclusion. Therefore, zeolite can be included in the diet of commercial layers at up to 0.93% without affecting their production performance or egg quality.