Effects of dietary soybean lecithin on growth performances, body composition, serum biochemical parameters, digestive and metabolic abilities in largemouth bass Micropterus salmoides

Yang, Wenxue; Wu, Jiaojiao; Song, Rui; Li, Zhe; Jia, Xiaowei; Qian, Pengcheng; Zhang, Hao; Zhang, Penghui; Xue, Xinyu; Li, Shenghui; Xie, Yuanyuan; Ye, Jinyun; Dong, Guancang; Wu, Chenglong

doi:10.1016/j.aqrep.2023.101528

Cited by 5 publications

(25 citation statements)

References 74 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In this study, AST activities were heightened in the serum of the GLU group, indicating that dietary GLU could impair liver health in largemouth bass to some extent. Moreover, endogenous hormones always play crucially regulatory functions in glucose metabolic process in fish species [17,34,35]. Generally, serum glucose level can be reduced by INS via increasing glucose uptake, while increased by GC via elevating glucose production in response to various external nutrients [36][37][38].…”

Section: Discussionmentioning

confidence: 99%

“…After the feeding trial, all fish were fasted for 24 h. The fish were initially anesthetized according to the information outlined in a previous study [17]. They were then weighted and measured for subsequent growth parameters [15][16][17]. Blood samples were first taken from the tail vein of 15 fish for next blood cell identification and counting.…”

Section: Sample Collectionmentioning

confidence: 99%

“…Blood cell identification and counting were conducted using a TEK 8500 VET automatic blood analyzer (Tekang Technology, Nanchang, China) following methods described by Wu et al [18]. Metabolite contents and enzyme activities in the serum samples were detected on C400n automated hematology analyzer (Shenzhen, China) according to the corresponding instructions described by Yang et al [17]. The contents variation of hormones modulating glucose metabolism and appetite in the serum samples were also measured using the commercial detecting kits (Jiancheng Biotech Co., Nanjing, China) following their corresponding instructions.…”

Section: Serum Cell Counting and Biochemical Indexesmentioning

confidence: 99%

“…The total RNA in liver was extracted using Trizol reagent (Invitrogen, Carlsbad, CA, USA). Subsequently, cDNA samples were generated using the RT-PCR kit (Monad Biotech, Wuhan, China), following the protocol published previously [17]. Furthermore, all cDNA samples were kept at −80 • C for further real-time quantitative PCR detection and analysis.…”

Section: Rna Extraction and Analysis Of Gene Expressionmentioning

confidence: 99%

“…Micropterus salmoides, commonly known as largemouth bass, has a great economic value in the Chinese market. In recent years, the production of largemouth bass has expanded rapidly due to its fast growth rate, strong adaptability, and high nutritional value [17]. More high-quality compound feeds will be needed to meet the rising production of largemouth bass.…”

Section: Introductionmentioning

confidence: 99%

See 4 more Smart Citations

Effects of Five Dietary Carbohydrate Sources on Growth, Glucose Metabolism, Antioxidant Capacity and Immunity of Largemouth Bass (Micropterus salmoides)

Qian,

Liu,

Zhang

et al. 2024

Animals

View full text Add to dashboard Cite

This study investigated the effects of glucose (GLU), tapioca starch (TS), gelatinized tapioca starch (GTS), potato starch (PS) and gelatinized potato starch (GPS) on growth and physiological responses in juvenile largemouth bass Micropterus salmoides. After 8 weeks, fish fed with starch diets had better weight gain and growth rates. Counts of red blood cells and monocytes were increased in the PS and GPS groups, compared to GLU group. Contents of serum triglyceride and total cholesterol were markedly elevated in the TS, PS and GPS groups. There were lower levels of serum glucose, insulin and cholecystokinin, and higher agouti-related peptide contents in the PS group compared to GLU group. PS and GPS could enhance glycolysis and TCA cycle by increasing their enzyme activities and transcriptional levels. Additionally, starch sources markedly heightened mRNA levels of key genes involved in the respiratory electron transport chain. Additionally, elevated mRNA levels of key antioxidant genes were shown in the TS and GTS groups. Moreover, TS and PS could promote immunity by upregulating transcriptional levels of the complement system, lysozyme and hepcidin. Taken together, starch exhibited better growth via increasing glycolysis and TCA cycle compared with GLU, and PS could improve antioxidant and immune capacities in largemouth bass.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Sample Collectionmentioning

confidence: 99%

Section: Serum Cell Counting and Biochemical Indexesmentioning

confidence: 99%

Section: Rna Extraction and Analysis Of Gene Expressionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Effects of Five Dietary Carbohydrate Sources on Growth, Glucose Metabolism, Antioxidant Capacity and Immunity of Largemouth Bass (Micropterus salmoides)

Qian,

Liu,

Zhang

et al. 2024

Animals

View full text Add to dashboard Cite

show abstract

Effects of selenoprotein extracts from Cardamine hupingshanensis on growth, selenium metabolism, antioxidant capacity, immunity and intestinal health in largemouth bass Micropterus salmoides

Zhang,

Zhao,

Zhang

et al. 2024

Front. Immunol.

Self Cite

View full text Add to dashboard Cite

This study aimed to assess the impact of dietary selenoprotein extracts from Cardamine hupingshanensis (SePCH) on the growth, hematological parameters, selenium metabolism, immune responses, antioxidant capacities, inflammatory reactions and intestinal barrier functions in juvenile largemouth bass (Micropterus salmoides). The base diet was supplemented with four different concentrations of SePCH: 0.00, 0.30, 0.60 and 1.20 g/Kg (actual selenium contents: 0.37, 0.59, 0.84 and 1.30 mg/kg). These concentrations were used to formulate four isonitrogenous and isoenergetic diets for juvenile largemouth bass during a 60-day culture period. Adequate dietary SePCH (0.60 and 1.20 g/Kg) significantly increased weight gain and daily growth rate compared to the control groups (0.00 g/Kg). Furthermore, 0.60 and 1.20 g/Kg SePCH significantly enhanced amounts of white blood cells, red blood cells, platelets, lymphocytes and monocytes, and levels of hemoglobin, mean corpuscular volume and mean corpuscular hemoglobin in the hemocytes. In addition, 0.60 and 1.20 g/Kg SePCH increased the mRNA expression levels of selenocysteine lyase, selenophosphate synthase 1, 15 kDa selenoprotein, selenoprotein T2, selenoprotein H, selenoprotein P and selenoprotein K in the fish liver and intestine compared to the controls. Adequate SePCH not only significantly elevated the activities of antioxidant enzymes (Total superoxide dismutase, catalase, glutathione reductase, glutathione peroxidase), the levels of total antioxidant capacity and glutathione, while increased mRNA transcription levels of NF-E2-related factor 2, Cu/Zn-superoxide dismutase, catalase, glutathione reductase and glutathione peroxidase. However, adequate SePCH significantly decreased levels of malondialdehyde and H2O2 and the mRNA expression levels of kelch-like ECH-associated protein 1a and kelch-like ECH-associated protein 1b in the fish liver and intestine compared to the controls. Meanwhile, adequate SePCH markedly enhanced the levels of immune factors (alkaline phosphatase, acid phosphatase, lysozyme, complement component 3, complement component 4 and immunoglobulin M) and innate immune-related genes (lysozyme, hepcidin, liver-expressed antimicrobial peptide 2, complement component 3 and complement component 4) in the fish liver and intestine compared to the controls. Adequate SePCH reduced the levels of pro-inflammatory cytokines (tumour necrosis factor-α, interleukin 8, interleukin 1β and interferon γ), while increasing transforming growth factor β1 levels at both transcriptional and protein levels in the liver and intestine. The mRNA expression levels of mitogen-activated protein kinase 13 (MAPK 13), MAPK14 and nuclear factor kappa B p65 were significantly reduced in the liver and intestine of fish fed with 0.60 and 1.20 g/Kg SePCH compared to the controls. Histological sections also demonstrated that 0.60 and 1.20 g/Kg SePCH significantly increased intestinal villus height and villus width compared to the controls. Furthermore, the mRNA expression levels of tight junction proteins (zonula occludens-1, zonula occludens-3, Claudin-1, Claudin-3, Claudin-5, Claudin-11, Claudin-23 and Claudin-34) and Mucin-17 were significantly upregulated in the intestinal epithelial cells of 0.60 and 1.20 g/Kg SePCH groups compared to the controls. In conclusion, these results found that 0.60 and 1.20 g/Kg dietary SePCH can not only improve growth, hematological parameters, selenium metabolism, antioxidant capacities, enhance immune responses and intestinal functions, but also alleviate inflammatory responses. This information can serve as a useful reference for formulating feeds for largemouth bass.

show abstract

Effects of Five Lipid Sources on Growth, Hematological Parameters, Immunity and Muscle Quality in Juvenile Largemouth Bass (Micropterus salmoides)

Song,

Yao,

Jing

et al. 2024

Animals

Self Cite

View full text Add to dashboard Cite

This study investigated the effects of fish oil (FO), soybean oil (SO), rapeseed oil (RO), peanut oil (PO) and lard oil (LO) on growth, immunity and muscle quality in juvenile largemouth bass. After 8 weeks, the results showed that FO and RO could increase weight gain and serum alkaline phosphatase and apelin values compared with LO (p < 0.05). Except lower crude lipid contents, higher amounts of n-3 polyunsaturated fatty acids (15.83% and 14.64%) were present in the dorsal muscle of the FO and RO groups. Meanwhile, FO and RO could heighten mRNA levels of immune defense molecules (lysozyme, hepcidin, and transforming growth factor β1) compared with PO (p < 0.05). While SO could increase potential inflammatory risk via rising counts of white blood cells, platelets, neutrophils and monocytes, and mRNA levels of interleukins (IL-1β, IL-8, IL-12 and IL-15), FO and RO could improve hardness, chewiness and springiness through increasing amounts of hydroxyproline, collagen and lysyl oxidase, and mRNA levels of collagen 1α2 and prolyl hydroxylase in the fish dorsal muscle. Moreover, FO and RO could improve firmness through increasing glycogen and glycogen synthase 1 levels when compared with LO (p < 0.05). Therefore, these results could provide dietary lipid source references during the feeding process of adult largemouth bass.

show abstract

Effects of dietary soybean lecithin on growth performances, body composition, serum biochemical parameters, digestive and metabolic abilities in largemouth bass Micropterus salmoides

Cited by 5 publications

References 74 publications

Effects of Five Dietary Carbohydrate Sources on Growth, Glucose Metabolism, Antioxidant Capacity and Immunity of Largemouth Bass (Micropterus salmoides)

Effects of Five Dietary Carbohydrate Sources on Growth, Glucose Metabolism, Antioxidant Capacity and Immunity of Largemouth Bass (Micropterus salmoides)

Effects of selenoprotein extracts from Cardamine hupingshanensis on growth, selenium metabolism, antioxidant capacity, immunity and intestinal health in largemouth bass Micropterus salmoides

Effects of Five Lipid Sources on Growth, Hematological Parameters, Immunity and Muscle Quality in Juvenile Largemouth Bass (Micropterus salmoides)

Contact Info

Product

Resources

About