Effects of dietary protein/carbohydrate ratio on fat deposition and gene expression of peroxisome proliferator activated receptor γ and heart fatty acid-binding protein of finishing pigs

Guo, Xianguang; Tang, Ruimin; Wang, Wei; Liu, Dayu; Wang, Kangning

doi:10.1016/j.livsci.2011.02.016

Cited by 30 publications

(31 citation statements)

References 32 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This variation observed in the LL muscle, but not in the SAT, of lean pigs fed low protein diets with high energy has been previously described by Guo et al (2011). The increase of intramuscular lipids in dietary protein-restricted growing pigs could have negative health implications, if a reduction in circulating insulin occurs.…”

Section: Discussionsupporting

confidence: 68%

Contrasting cellularity on fat deposition in the subcutaneous adipose tissue and longissimus lumborum muscle from lean and fat pigs under dietary protein reduction

Lopes

Costa

et al. 2014

Animal

View full text Add to dashboard Cite

The production of pork with high amounts of intramuscular fat (IMF) without an increase in subcutaneous fat is highly desirable for the pig industry and consumers. Herein, we question the impact of dietary protein reduction (18% v. 13%) on fat deposition in the subcutaneous adipose tissue (SAT) and longissimus lumborum (LL) muscle using genetically diverse pigs for body fatness (lean v. fat). A clear effect of genotype was observed on plasma insulin (P = 0.004) and leptin (P < 0.001), as well as on backfat thickness (P < 0.001), with the fat pigs having higher values. Accordingly, IMF was higher in the fat pigs, when compared with their lean counterparts (P = 0.003), which was supported by enlarged adipocytes (P < 0.001). The area of lipid droplets within the LL fibres (P = 0.039) and extramyocellular lipids number (P = 0.017) were increased in pigs fed reduced protein diets, regardless of genotype, which is consistent with higher levels of plasma triacylglycerols (P = 0.002). The gene-expression pattern of lipogenic factors in the SAT was distinct from the LL muscle. In the SAT, PPARG expression was similar among genotypes (P > 0.05), whereas in the LL muscle it was higher in the lean pigs (P = 0.023), especially when fed on low protein diet (P = 0.057). The CEBPA and FABP4 mRNA levels were increased in the SAT of fat pigs (P < 0.001), without changes in the LL muscle (P > 0.05). The influence of diet on FABP4 expression in the SAT was dependent on pig's genetic background (P = 0.005). In conclusion, fat deposition was clearly influenced by genotype and, to a lesser extent, by dietary protein level, the SAT being more sensitive than the LL muscle. One can speculate that the pathways involved in lipid metabolism are downregulated in intramuscular adipocytes when compared with SAT fat cells. This result might be a direct consequence of the relatively low proportion of adipocytes found in the LL muscle.

show abstract

Section: Discussionsupporting

confidence: 68%

Contrasting cellularity on fat deposition in the subcutaneous adipose tissue and longissimus lumborum muscle from lean and fat pigs under dietary protein reduction

Lopes

Costa

et al. 2014

Animal

View full text Add to dashboard Cite

show abstract

“…These results suggest that the protein restriction has higher effect on modern breeds (Large White and Duroc) than in traditional breeds (Berkshire and Tamworth; Wood et al, 2008). In addition, these authors attribute the response to the breed-specific expression of muscle fibre types and key lipogenic enzymes, which have been described as the most important factors influencing IMF in pigs (Guo et al, 2011). Other reasons for the discrepancies among experiments may be related to diet composition or degree of AA restriction.…”

Section: Meat Characteristicsmentioning

confidence: 94%

Effects of sex and dietary lysine on performances and serum and meat traits in finisher pigs

Suárez-Belloch

Guada

Latorre

2015

Animal

View full text Add to dashboard Cite

A total of 160 Duroc × (Landrace × Large White) pigs, 50% barrows and 50% gilts, of 28.3 ± 4.52 kg of BW were used to study the effect of lysine (Lys) restriction in the finisher period, on growth performances and serum and carcass and meat quality traits. The grower diet (from 30 to 90 kg BW) was slightly Lys-restricted (7.8 g standardised ileal digestible (SID) Lys/kg) in accordance with results from a previous trial. During the finisher period (90 to 130 kg BW), four experimental diets with decreasing SID Lys contents (6.3, 5.6, 4.2 and 3.2 g/kg) were tested. Each of the eight treatments (two sexes × four levels of Lys) was replicated five times. Each replicate was a pen with four pigs allocated together. When animals achieved 129 ± 2.59 kg were slaughtered and carcass and meat characteristics were evaluated. No significant interaction sex × diet was found. During the finisher period, barrows grew faster ( P < 0.001) and ate more feed ( P < 0.001) but tended to be less efficient ( P = 0.055) than gilts. The Lys restriction affected linearly ( P < 0.001) all productive performance traits; daily BW gain and feed intake decreased and feed conversion ratio increased. Also, the concentration of serum urea at slaughter tended to be higher in barrows than in gilts ( P = 0.065) and was reduced quadratically by the restriction of Lys in feed ( P < 0.001). Carcasses from barrows had higher backfat thickness ( P < 0.01) and lower weight of main trimmed lean cuts (ham + shoulder + loin; P < 0.05) than those from gilts. The Lys restriction during the finisher period decreased carcass yield (quadratic; P < 0.001) and the weight of major cuts (linear; P < 0.001). Sex and diet had limited effect on meat characteristics; the Lys restriction decreased quadratically the proportion of protein ( P < 0.01) and increased linearly the intramuscular fat (IMF) content ( P < 0.001). We can conclude that dietary Lys restriction during finisher period in pigs impaired growth performances and was not successful to increase the carcass fat deposition, although it could have positive effects on IMF proportion of pork.

show abstract

“…Each chop was then sheared four times at a crosshead speed of 1 mm/s using a Texture Measurement System (Food Technology Corporation). Warner Bratzler shear force (WBSF) for individual chops were averaged for each sample, and then were averaged five replicates for each dietary treatment (Guo et al 2011). …”

Section: Samples Collection and Determination Of Muscle Propertymentioning

confidence: 99%

Effects of dietary α-lipoic acid on carcass characteristics, antioxidant capability and meat quality in Hainan black goats

Wang

Zhou

et al. 2016

Italian Journal of Animal Science

View full text Add to dashboard Cite

The effects of a-lipoic acid (LA) on the carcase characteristics, antioxidant capability and meat quality of Hainan black goat were investigated in the present study. Thirty goats (10.71 ± 0.42 kg) were randomly assigned with three levels of LA in the concentration diet (0, 300, 600 mg/kg). The goats fed for 70 days. The results showed that goats fed the diet containing 600 mg/kg LA had a significantly (p < .05) higher ADG and better feed conversion rate (FCR) compared with goats offered the basal diet without LA. There were no differences in carcase characteristics among the three treatments. For antioxidant enzymes, glutathione peroxidase (GSH-Px), superoxide dismutase (SOD), total antioxidant capacity (T-AOC) and catalase (CAT) in serum significantly increased (p < .05) in goats fed with 600 mg/kg LA at day 70. The concentration of malondiadehhyde (MDA) was significantly lower (p < .05) in serum of goats that received 600 mg/kg LA than in the goats fed diet without LA. Addition level of LA had no significant impact on pH and intramuscular fat (IMF) of longissimus dorsi muscle tissue (LM) and semimembranosus muscle tissue (SM) in the goats (p > .05). However, increasing additional LA in the diet caused a decrease on drip loss and WBSF (p < .05). Treatment of goats with 600 mg/kg of LA has the lowest drip loss and WBSF. These results indicate that diet supplementation with LA may enhance antioxidant capability, improved the growth performance and meat quality in Hainan black goats. ARTICLE HISTORY

show abstract

Effects of dietary protein/carbohydrate ratio on fat deposition and gene expression of peroxisome proliferator activated receptor γ and heart fatty acid-binding protein of finishing pigs

Cited by 30 publications

References 32 publications

Contrasting cellularity on fat deposition in the subcutaneous adipose tissue and longissimus lumborum muscle from lean and fat pigs under dietary protein reduction

Contrasting cellularity on fat deposition in the subcutaneous adipose tissue and longissimus lumborum muscle from lean and fat pigs under dietary protein reduction

Effects of sex and dietary lysine on performances and serum and meat traits in finisher pigs

Effects of dietary α-lipoic acid on carcass characteristics, antioxidant capability and meat quality in Hainan black goats

Contact Info

Product

Resources

About