Effects of dietary potassium diformate (KDF) on growth performance, feed conversion and intestinal bacterial community of hybrid tilapia (Oreochromis niloticus ♀×O. aureus ♂)

Zhou, Zhigang; Liu, Yuchun; He, Shunping; Shi, Pengjun; Gao, Xiuhua; Yao, Bin; Ringø, Einar

doi:10.1016/j.aquaculture.2009.02.043

Cited by 116 publications

(111 citation statements)

References 29 publications

Supporting

Mentioning

100

Contrasting

Unclassified

Order By: Relevance

“…Finally, all representative sequences were taxonomically classified with the RDP classifier using an 80% confidence threshold (Wang, Garrity, Tiedje & Cole 2007). The relative abundance (%) of OTUs, representing the ratio of the number of the clones of a specific OTU to the total number of clones, was considered to be significant when the value was more than 1.5-fold higher or less than 0.5-fold lower than the abundance of any other OTU (Zhou, Liu, He, Shi, Gao, Yao & Ringø 2009). Estimates of phylotype richness (Schao 1 index), diversity (Shannon-Weaver index), dominance concentration (Simpson index) and evenness (equitability index) were calculated based on the 16S rDNA phylotype data respectively (Brown & Bowman 2001).…”

Section: Discussionmentioning

confidence: 99%

Diversity of autochthonous bacterial communities in the intestinal mucosa of grass carp (Ctenopharyngodon idellus) (Valenciennes) determined by culture-dependent and culture-independent techniques

Zhong

Wirth

et al. 2014

Aquac Res

View full text Add to dashboard Cite

Traditional culture-based technique and 16S rDNA sequencing method were used to investigate the mucosa-associated autochthonous microbiota of grass carp (Ctenopharyngodon idellus). Twenty-one phylotypes were detected from culturable microbiota, with Aeromonas, Shewanella, Lactococcus, Serratia, Brevibacillus, Delftia, Pseudomonas, Pantoea, Enterobacter, Buttiauxella and Yersinia as their closest relatives. Genomic DNA was directly extracted from the gut mucosa of C. idellus originating from six different geographical regions, and used to generate 609 random bacterial clones from six clone libraries and 99 archaeal clones from one library, which were grouped into 67 bacterial and four archaeal phylotypes. Sequence analysis revealed that the intestinal mucosa harboured a diversified bacterial microbiota, where Proteobacteria, Firmicutes and Bacteroidetes were dominant, followed by Actinobacteria, Verrucomicrobia and DeinococcusThermus. The autochthonous bacterial communities in the gut mucosa of fish from different aquatic environments were not similar (C s < 0.80), but c-Proteobacteria was a common bacterial class. In comparison to bacterial communities, the archaeal community obtained from one library consisted of Crenarchaeota and Euryarchaeota. These results demonstrate that molecular methods facilitate culture-independent studies, and that fish gut mucosa harbours a larger bacterial diversity than previously recognized. The grass carp intestinal habitat selects for specific bacterial taxa despite pronounced differences in host environments.

show abstract

Section: Discussionmentioning

confidence: 99%

Diversity of autochthonous bacterial communities in the intestinal mucosa of grass carp (Ctenopharyngodon idellus) (Valenciennes) determined by culture-dependent and culture-independent techniques

Zhong

Wirth

et al. 2014

Aquac Res

View full text Add to dashboard Cite

show abstract

“…Nevertheless, the basal composition of the gut microbiome of important economic species such as Nile tilapia Oreochromis niloticus L. is still largely unknown. Several studies have analyzed the effects of different feed additives in the intestinal bacterial community of tilapia; however, those studies employed a fingerprint-based method (DGGE) suitable for identifying changes in the communities but without describing species composition (Pedrotti et al, 2015, He et al, 2011, Zhou et al, 2009, Liu et al, 2014.…”

Section: Introductionmentioning

confidence: 99%

Analysis of the Gut Microbiome of Nile Tilapia Oreochromis Niloticus L. Fed Diets Supplemented with Previda® and Saponin

Ray¹,

Buján²,

Tarnecki³

et al. 2017

FisheriesSciences.com

View full text Add to dashboard Cite

Abstract:The use of prebiotics in aquaculture aims at increasing the numbers of beneficial microbes in the gastrointestinal tract of fishes. However, the true effect of prebiotics exert on the fish microbiome is for the most part unknown. This study aimed at investigating the effects of the commercial prebiotic Previda® and the feed supplement saponin on Nile tilapia (Oreochromis niloticus L.). We used 454 pyrosequencing to characterize the gut microbiome of fish fed diets containing Previda®, saponin, a combination of both, and a control diet. After data normalization, 98,300 sequence reads were analyzed resulting in 331 unique operational taxonomic units (OTUs). Three phyla were present in all samples: Fusobacteria, Bacteroidetes, and Proteobacteria. Fusobacteria was the predominant phylum in all samples accounting for more than 80% of the OTUs. Within the Fusobacteria, the predominant genus was Cetobacterium with more than 76% of the sequences being ascribed to that species. Barnesiella was the second most common genus with more than 6% of the total sequences. Overall, there was not a significant difference in the composition of the gut microbiomes regardless of the diet. The overwhelming numbers of Cetobacterium sp. present in the gut of Nile tilapia could overshadow the effect of the treatments on other genera.

show abstract

“…Scheppach (1994) showed that in domestic animal, VFAs stimulated the colonic sodium and fluid absorption and exerted a proliferative effect on the colonocytes. In fish, the beneficial effects of VFA resulted in strong antimicrobial activity, growth promotion (Elala and Ragaa, 2015, Ng et al, 2009, Zhou et al, 2009) and improvement in villi development in the intestine, leading to a better nutrient utilization (Robles et al, 2013). Our study showed a positive correlation between NSP content and the widening of the LP and the increase of the number of GC.…”

Section: The Correlation Between Nutrient Content Nutrient Digestibimentioning

confidence: 49%

“…Some studies found no effect of OA on growth rate (Owen et al, 2006, Zhou et al, 2009, Gao et al, 2011, Zhu et al, 2014 whereas others did find an effect (Ramli et al, 2005, Liebert et al, 2010, Castillo et al, 2014. Lim et al (2010) stated that the variability in OA effects on growth between studies depends on fish species, age, and the types and levels of organic acids used.…”

Section: Discussionmentioning

confidence: 99%

“…The possible mode of action of organic acids in the intestinal tract involves two different mechanisms: 1) it reduces the pH level in the stomach and in the small intestine, through the delivery of H + ions, 2) it inhibits growth of Gram-negative bacteria through the dissociation of the acids and the production of anions inside bacterial cells (Lückstädt, 2007). Studies on the use of organic acids in tilapia diets are numerous, however, between studies results are often highly variable, both in type of response and significance (Zhou et al, 2009, Ng et al, 2009, Hassaan et al, 2014, Elala and Ragaa, 2015, Ramli et al, 2005.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Feeds, water quality, gut morphology and digestion in Nile tilapia (Oreochromis niloticus)

Kim¹

View full text Add to dashboard Cite

show abstract

Effects of dietary potassium diformate (KDF) on growth performance, feed conversion and intestinal bacterial community of hybrid tilapia (Oreochromis niloticus ♀×O. aureus ♂)

Cited by 116 publications

References 29 publications

Diversity of autochthonous bacterial communities in the intestinal mucosa of grass carp (Ctenopharyngodon idellus) (Valenciennes) determined by culture-dependent and culture-independent techniques

Diversity of autochthonous bacterial communities in the intestinal mucosa of grass carp (Ctenopharyngodon idellus) (Valenciennes) determined by culture-dependent and culture-independent techniques

Analysis of the Gut Microbiome of Nile Tilapia Oreochromis Niloticus L. Fed Diets Supplemented with Previda® and Saponin

Feeds, water quality, gut morphology and digestion in Nile tilapia (Oreochromis niloticus)

Contact Info

Product

Resources

About