Effects of Dietary Phosphorus Levels on Growth Performance, Phosphorus Utilization and Intestinal Calcium and Phosphorus Transport-Related Genes Expression of Juvenile Chinese Soft-Shelled Turtle (Pelodiscus sinensis)

Wang, Yue; Geng, Yiran; Shi, Xueying; Wang, Siqi; Yang, Zhencai; Zhang, Peiyu; Liu, Haiyan

doi:10.3390/ani12223101

Cited by 9 publications

(11 citation statements)

References 66 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Higher diet intake equates to faster digesta transit, which can result in a lower digestive efficiency [ 63 ]. Therefore, the reduction of AD m and AD e of A. japonicus in the present study may result from high feed intake, which agrees with the report on turtle Pelodiscus sinensis [ 64 ]. In addition, apparent digestibility was also indicator of the balance of nutrient deposition and excretion.…”

Section: Discussionsupporting

confidence: 92%

“…Induction and secretion of phosphatase is one of the important adaptive responses of the animal to low phosphorus status. Studies with abalone [ 25 ] and turtle Pelodiscus sinensis [ 64 ] demonstrated that the tissue activity of alkaline phosphatase is negatively correlated with dietary phosphorus levels. However, the present study showed that dietary phosphorus-stimulated intestinal phosphatase activity as dietary phosphorus level increased up to 0.62%.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Influence of Dietary Phosphorus on the Growth, Feed Utilization, Proximate Composition, Intestinal Enzymes, and Oxidation Resistance of Sea Cucumber Apostichopus japonicus

Song

et al. 2023

Aquaculture Nutrition

View full text Add to dashboard Cite

Six experimental diets (crude protein 12.58%, crude fat 1.93%, and total energy 10.72 kJ/kg) containing 0.24%, 0.37%, 0.51%, 0.62%, 0.77%, and 0.89% phosphorus were formulated to evaluate dietary phosphorus requirement for sea cucumber Apostichopus japonicus. The feeding trial was conducted in 18 fiberglass tanks (220 L) for 63 days. Each diet was randomly assigned to triplicate tanks of 50 sea cucumbers (9.99 g) and fed once daily. With the increase of dietary phosphorus level, weight gain (WG), specific growth rate (SGR), daily feed intake (DFI), feces production ratio, the activities of amylase, alkaline phosphatase, phosphofructokinase, succinate dehydrogenase, and glutathione peroxidase as well as the contents of glutathione and glutathione oxidized significantly increased and then decreased afterwards ( P < 0.05 ). A. japonicus fed diet with 0.63%, 0.63%, and 0.55% dietary phosphorus was estimated to yield the highest WG (11.39 g), SGR (1.09%/d), and DFI (2.55%/d) according to the quadratic regression analysis of WG, SGR, and DFI against dietary phosphorus level, respectively. The apparent digestibility of dry material and energy followed an opposite tendency. Feed efficiency, the contents of whole-body phosphorus, initially increased and then plateaued, fitting piecewise-linear models with breakpoint at 0.57% and 0.55% dietary phosphorus. Daily phosphorus intake, pyruvate kinase activity, and the ratio of glutathione and glutathione oxidized increased ( P < 0.05 ) but the apparent digestibility of phosphorus, the activities of alkaline protease, aspartate transaminase, and phosphoenolpyruvate carboxykinase decreased ( P < 0.05 ), responding to the increasing dietary phosphorus. Considering the present results, the optimal dietary phosphorus for A. japonicus is 0.57–0.63%.

show abstract

Section: Discussionsupporting

confidence: 92%

Section: Discussionmentioning

confidence: 99%

Influence of Dietary Phosphorus on the Growth, Feed Utilization, Proximate Composition, Intestinal Enzymes, and Oxidation Resistance of Sea Cucumber Apostichopus japonicus

Song

et al. 2023

Aquaculture Nutrition

View full text Add to dashboard Cite

show abstract

“…It facilitates the hydrolysis of phosphate into phosphoric acid under alkaline conditions. Various forms of ALP, including small intestine ALP and bone-type ALP, contribute to the regulation of intestinal function, as well as calcium and phosphorus metabolism [44,45]. DiLorenzo and McCarthy, among others, have provided evidence suggesting that AMY may potentially accelerate the rate of ruminal carbohydrate degradation [46,47].…”

Section: Discussionmentioning

confidence: 99%

Effects of High-Concentrate-Induced SARA on Antioxidant Capacity, Immune Levels and Rumen Microbiota and Function in Goats

Fan,

Zheng,

Ren

et al. 2024

Animals

View full text Add to dashboard Cite

This study aims to explore the antioxidant, immune, and enzyme metabolism aspects in goats experiencing subacute ruminal acidosis (SARA). Furthermore, we seek to elucidate the relationship between the symbiotic microbiota of goats and their metabolic function. Sixteen goats were equally divided into two groups and fed a normal-concentrate diet (NC, 55% concentrate) or a high-concentrate diet (HC, 90% concentrate) for five weeks. We found that the HC diet reduced the total antioxidant capacity (T-AOC) (p = 0.022) and increased interleukin-1β (IL-1β) (p = 0.015), interleukin-4 (IL-4) (p = 0.008) and interleukin-6 (IL-6) (p = 0.002) concentration of goats. Simultaneously, the HC diet significantly increased the concentrations of alkaline phosphatase (ALP) and amylase (AMY) in the blood and rumen fluid of goats (p < 0.05). Microbial analysis in the rumen of goats revealed that the HC diet decreased bacterial richness and diversity, as evidenced by the changed observed species, Chao 1, PD whole tree and Shannon when compared to the NC diet (p < 0.01). The proportion of Proteobacteria increased while that of Spirochaetes and Fibrobacteres significantly decreased with the HC diet (p < 0.05). The Christensenellaceae_R-7_group and Ruminococcaceae_UCG-010 in rumen was notably decreased when a diet was switched from 55% concentrate diet to 90% concentrate diet (p < 0.05). Additionally, microbial functional potentials deduced that the HC diet significantly increased the abundance of the citrate cycle (TCA cycle) (ko00020) associated with carbohydrate metabolism (p = 0.028). Furthermore, the HC diet significantly increased the glutathione metabolism (ko00480) associated with the metabolism of other amino acids (p = 0.008). Our findings suggested that SARA reduced the total antioxidant capacity and increased levels of inflammatory factors in goats, as well as decreased rumen bacterial species and abundance.

show abstract

“…The main nutritional components of the enzymatic cottonseed protein used in the experiment are 62% crude protein and 0.4% crude lipid. The formula was designed according to the nutritional requirements of Chinese soft-shelled turtle [27][28][29][30]. The experimental feed was prepared with fish meal as the protein source, wheat meal as the sugar source, and soybean oil as the fat source.…”

Section: Experimental Ingredients and Dietsmentioning

confidence: 99%

Effects of Replacing Fish Meal with Enzymatic Cottonseed Protein on the Growth Performance, Immunity, Antioxidation, and Intestinal Health of Chinese Soft-Shelled Turtle (Pelodiscus sinensis)

Qiu

Zhao

Xie

et al. 2023

Aquaculture Nutrition

View full text Add to dashboard Cite

The dietary effects of replacing fish meal with enzymatic cottonseed protein (ECP) on the growth performance, immunity, antioxidant, and intestinal health of Chinese soft-shelled turtles have not been explored. An eight-week feeding trial was conducted with a quadruplicated group of turtles ( 3.44 ± 0.01 g ) that were randomly assigned to 16 cages ( 0.6 m × 0.6 m × 0.6 m ) with 30 turtles that were stocked in each cage. Four dietary groups were fed with diets supplemented with 0, 2%, 4%, and 6% (ECP0 group (control group), ECP2 group, ECP4 group, ECP6 group) of enzymatic cottonseed protein replacing fishmeal. The present study illustrated that the final weight and WG in the ECP2 and ECP4 groups were significantly increased ( P < 0.05 ) compared with the control group. The ECP2, ECP4, and ECP6 groups significantly reduced the feed coefficient ( P < 0.05 ) and significantly increased the SGR ( P < 0.05 ). The serum TP and ALB of the ECP4 group were significantly increased ( P < 0.05 ). The ECP2, ECP4, and ECP6 groups significantly increased the activity of intestinal pepsin ( P < 0.05 ), and the activity of intestinal lipase of the EPC4 group was significantly increased ( P < 0.05 ). The intestinal villus height of the EPC4 group and EPC6 group, the villus width of the EPC2 group and EPC4 group, and the intestinal muscle thickness of the EPC4 group were significantly increased ( P < 0.05 ). At the same time, replacing fishmeal with enzymatic cottonseed protein also affected the intestinal inflammation-related genes compared with the control group. Besides that, the expression of the IL-10 gene in the experimental group was significantly upregulated ( P < 0.05 ). Nevertheless, the expression of TNF-α and IL-8 genes in the ECP2 group and TNF-α and IL-1β genes in the ECP4 group was significantly downregulated ( P < 0.05 ). In summary, replacing fish meal with enzymatic cottonseed protein positively affects the growth, immunity, and intestinal health of Chinese soft-shelled turtles. The appropriate proportion of enzymatic cottonseed protein to replace fish meal in turtle feed is 4%.

show abstract

Effects of Dietary Phosphorus Levels on Growth Performance, Phosphorus Utilization and Intestinal Calcium and Phosphorus Transport-Related Genes Expression of Juvenile Chinese Soft-Shelled Turtle (Pelodiscus sinensis)

Cited by 9 publications

References 66 publications

Influence of Dietary Phosphorus on the Growth, Feed Utilization, Proximate Composition, Intestinal Enzymes, and Oxidation Resistance of Sea Cucumber Apostichopus japonicus

Influence of Dietary Phosphorus on the Growth, Feed Utilization, Proximate Composition, Intestinal Enzymes, and Oxidation Resistance of Sea Cucumber Apostichopus japonicus

Effects of High-Concentrate-Induced SARA on Antioxidant Capacity, Immune Levels and Rumen Microbiota and Function in Goats

Effects of Replacing Fish Meal with Enzymatic Cottonseed Protein on the Growth Performance, Immunity, Antioxidation, and Intestinal Health of Chinese Soft-Shelled Turtle (Pelodiscus sinensis)

Contact Info

Product

Resources

About