Effects of Dietary Phosphate on Ectopic Calcification and Muscle Function in mdx Mice

Wada, Eitaro; Kikkawa, Namiko; Yoshida, Motoaki; Date, Munehiro; Higashi, T; Matsuda, Ryoichi

doi:10.5772/29510

Cited by 1 publication

(1 citation statement)

References 32 publications

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“…High-resolution CT has been previously used to detect ectopic calcifications in mdx mice [23][24][25], which is accentuated by high-phosphorus diet. In our experiments in mice on a normal diet, we detected significant (p G 0.01) differences in ectopic calcification between mdx mice and controls with CT, and the pattern of the ectopic calcification agreed with that shown in a previous work [25]. The pattern of ectopic calcification measured by SPECT was much more striking than that seen with CT.…”

Section: Discussionmentioning

confidence: 99%

SPECT Imaging of Muscle Injury with [99mTc]MDP in a Mouse Model of Muscular Dystrophy

et al. 2019

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Purpose: Tc-99m methylene diphosphonate ([ 99m Tc]MDP) is an in vivo bone imaging agent that also accumulates in injured skeletal muscle cells. The objective of this study was to investigate if [ 99m Tc]MDP could be used to detect muscle injury in the mdx mouse model of Duchenne muscular dystrophy (DMD). Procedures: Static whole-body single-photon emission computed tomography/computed tomography (CT) scans were acquired at 2 h post-injection of [ 99m Tc]MDP in two cohorts of animals at different sites: one cohort of mice at 6, 15, and 19 weeks of age, and a separate cohort at 16 weeks. The second cohort was also imaged with high-resolution CT at 8 weeks. Results: mdx mice had higher [ 99m Tc]MDP uptake and significantly higher [ 99m Tc]MDP concentrations in muscle than controls. Conclusions: Higher uptake of [ 99m Tc]MDP in muscle of mdx mice agrees with histological reports of muscle calcification in mdx mice, and suggests the potential translational use of [ 99m Tc]MDP imaging for tracking DMD progression and therapeutic response.

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Section: Discussionmentioning

confidence: 99%