Effects of Dietary N-Carbamylglutamate on Growth Performance, Apparent Digestibility, Nitrogen Metabolism and Plasma Metabolites of Fattening Holstein Bulls

Yang, Jiajun; Zheng, Jian; Fang, Xinpeng; Jiang, Xin; Sun, Yukun; Zhang, Yonggen

doi:10.3390/ani11010126

Cited by 13 publications

(13 citation statements)

References 34 publications

(17 reference statements)

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“…Then, all the feed samples were dried at 55 • C for 48 h, ground to pass through a 1-mm screen, and stored for chemical analysis. Fecal samples (about 500 g fresh) were collected from the rectum of individual animal daily at 600, 1,200, 1,800, and 2,400 h from d 28 to 30, and from d 58 to 60 according to the method of Yang et al (15). The collected fecal samples were pooled by animal and by sampling period, dried at 55 • C for 48 h, and ground to pass through a 1-mm screen for subsequent chemical analysis.…”

Section: Sample Collectionmentioning

confidence: 99%

Effects of supplementation with lysophospholipids on performance, nutrient digestibility, and bacterial communities of beef cattle

Zhang

Bai²,

Zhao³

et al. 2022

Front. Vet. Sci.

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An experiment was conducted to investigate the influences of supplemental lysophospholipids (LPL) on the growth performance, nutrient digestibility, and fecal bacterial profile, and short-chain fatty acids (SCFAs) of beef cattle. Thirty-six Angus beef cattle [565 ± 10.25 kg body weight (BW)] were grouped by BW and age, and randomly allocated to 1 of 3 treatment groups: (1) control (CON, basal diet); (2) LLPL [CON supplemented with 0.5 g/kg LPL, dry matter (DM) basis]; and (3) HLPL (CON supplemented with 0.75 g/kg, DM basis). The Angus cattle were fed a total mixed ration that consisted of 25% roughage and 75% concentrate (dry matter [DM] basis). The results reveal that LPL inclusion linearly increased the average daily gain (P = 0.02) and the feed efficiency (ADG/feed intake, P = 0.02), while quadratically increasing the final weight (P = 0.02) of the beef cattle. Compared with CON, the total tract digestibilities of DM (P < 0.01), ether extract (P = 0.04) and crude protein (P < 0.01) were increased with LPL supplementation. At the phylum-level, the relative abundance of Firmicutes (P = 0.05) and ratio of Firmicutes: Bacteroidetes (P = 0.04) were linearly increased, while the relative abundances of Bacteroidetes (P = 0.04) and Proteobacteria (P < 0.01) were linearly decreased with increasing LPL inclusion. At the genus-level, the relative abundances of Clostridium (P < 0.01) and Roseburia (P < 0.01) were quadratically increased, and the relative abundances of Ruminococcus was linearly increased (P < 0.01) with LPL supplementation. Additionally, increasing the dose of LPL in diets linearly increased the molar proportion of butyrate (P < 0.01) and total SCFAs (P = 0.01) concentrations. A conclusion was drawn that, as a promising feed additive, LPL promoted growth performance and nutrient digestibility, which may be associated with the change of fecal microbiome and SCFAs.

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Section: Sample Collectionmentioning

confidence: 99%

Effects of supplementation with lysophospholipids on performance, nutrient digestibility, and bacterial communities of beef cattle

Zhang

Bai²,

Zhao³

et al. 2022

Front. Vet. Sci.

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“… 41 NCG is used as an important feed additive in dairy cows, beef cattle, neonatal pigs, and mutton sheep to increase milk production, enhance the pregnancy rate, reduce morbidity and mortality, and increase birth weight and growth rate. 42–46 However, natural synthesis of NCG has not been reported. Therefore, the beneficial gut microbe L. reuteri DS0384, which produces NCG, may be useful for improving the health of people and livestock.…”

Section: Discussionmentioning

confidence: 99%

Limosilactobacillus reuteri DS0384 promotes intestinal epithelial maturation via the postbiotic effect in human intestinal organoids and infant mice

et al. 2022

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Little is known about the modulatory capacity of the microbiota in early intestinal development. We examined various intestinal models that respond to gut microbial metabolites based on human pluripotent stem cell-derived human intestinal organoids (hIOs): physiologically relevant in vitro fetal-like intestine, intestinal stem cell, and intestinal disease models. We found that a newly isolated Limosilactobacillus reuteri strain DS0384 accelerated maturation of the fetal intestine using 3D hIO with immature fetal characteristics. Comparative metabolomic profiling analysis revealed that the secreted metabolite N-carbamyl glutamic acid (NCG) is involved in the beneficial effect of DS0384 cell-free supernatants on the intestinal maturation of hIOs. Experiments in an intestinal stem cell spheroid model and hIO-based intestinal inflamed model revealed that the cell-free supernatant from DS0384 comprising NCG promoted intestinal stem cell proliferation and was important for intestinal protection against cytokine-induced intestinal epithelial injury. The probiotic properties of DS0384 were also evaluated, including acid and bile tolerance and ability to adhere to human intestinal cells. Seven-day oral administration of DS0384 and cell-free supernatant promoted the intestinal development of newborn mice. Moreover, NCG exerted a protective effect on experimental colitis in mice. These results suggest that DS0384 is a useful agent for probiotic applications and therapeutic treatment for disorders of early gut development and for preventing intestinal barrier dysfunction.

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“…In conclusion, the addition of NCG improved both the nitrogen utilization of the urea diet and consequently the ADG, while alleviating the difficult problem of protein feed shortage, which was supported by the synergistic effect between the urea diet and NCG. A previous study by our group suggested that supplementing Holstein bulls with 40 mg/kg of BW NCG was prior to increase growth performance compared with other doses [ 21 ]. Previous studies showed that dietary NCG had a beneficial effect on the feed efficiency of broilers and pigs [ 22 , 23 ].…”

Section: Discussionmentioning

confidence: 99%

Effects of Inclusion of N-Carbamylglutamate in the Non-Protein Diet on Growth and Slaughter Performance, Meat Quality, Nitrogen Metabolism and Antioxidant of Holstein Bulls

Zhang

et al. 2021

Animals

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The objectives of this experiment were to investigate the effects of N-carbamylglutamate (NCG) on growth and slaughter performance, meat quality, nitrogen utilization, plasma antioxidant and amino acids of Holstein bulls. In this case, 24 Holstein bulls (490 ± 29.0 kg of body weights and 540 ± 6.1 d of age) were blocked by body weights and age and randomly assigned to 1 of 4 groups: (1) CON group: bulls were fed the control diet, (2) CON + NCG group: bulls were fed the control diet with 40 mg/kg BW NCG, (3) Urea group: bulls were fed the urea diet, and (4) Urea + NCG group: bulls were fed the urea diet with 40 mg/kg BW NCG. Feeding NCG significantly improved ADG, FCR, DM and CP digestibility, carcass weight, slaughter weight, DOP, eye muscle area, shear force (p = 0.001) and reduced L* of color, drip loss and cooking loss. Concurrently, feeding the urea diet induced a decreased ADG, carcass weight and slaughter weight, DOP, eye muscle area and shear force. NCG decreased contents of fecal N and urinary N, plasma urea in bulls and ammonia but increased N retention and utilization, plasma NO, plasma Arg, Leu, Ile and Tyr. On the other hand, feeding the urea diet increased urinary N, plasma urea and ammonia. Thus the study efficiently demonstrates that beef benefited from being fed a NCG product in the urea diet by enhancing its growth and slaughter performance, meat quality, nitrogen metabolism and plasma amino acids.

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Effects of Dietary N-Carbamylglutamate on Growth Performance, Apparent Digestibility, Nitrogen Metabolism and Plasma Metabolites of Fattening Holstein Bulls

Cited by 13 publications

References 34 publications

Effects of supplementation with lysophospholipids on performance, nutrient digestibility, and bacterial communities of beef cattle

Effects of supplementation with lysophospholipids on performance, nutrient digestibility, and bacterial communities of beef cattle

Limosilactobacillus reuteri DS0384 promotes intestinal epithelial maturation via the postbiotic effect in human intestinal organoids and infant mice

Effects of Inclusion of N-Carbamylglutamate in the Non-Protein Diet on Growth and Slaughter Performance, Meat Quality, Nitrogen Metabolism and Antioxidant of Holstein Bulls

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