Effects of Dietary Methionine or Arginine Levels on the Urinary Creatinine Excretion in Broiler Chicks

Abstract

Two experiments were conducted to evaluate the usefulness of urinary creatinine levels as a criterion for the estimation of protein and amino acid requirements in poultry. Here we studied the effects of dietary precursor levels of creatinine, methionine and arginine, on urinary creatinine excretion in experiments. Both experiments used 15 Chunky broilers chicks that were 8 days old. The chicks were assigned to three dietary groups, with five chicks each, and were fed an experimental diet for 7 days. The experimental diets mainly consisted of corn and soybean meal, and contained deficient, adequate, or excessive methionine and arginine levels in experiments 1 and 2, respectively. Excreta were collected for the last 3 days of the feeding trial, and chicks were terminated by dislocation of the neck at the end of the feeding trial to collect their livers. Creatinine concentration in the excreta and hepatic L-arginineglycine amidinotransferase (AGAT) activities were determined.Urinary creatinine levels increased with increasing both dietary methionine and arginine levels from deficient to adequate recommended by Japanese feeding standard (P＜0.05), and then remained constant in experiments 1 and 2, respectively. The hepatic AGAT activity decreased when both dietary creatinine precursors levels were increased from deficient to adequate levels (p＜0.05), and then remained constant.These results suggested that creatinine excretion was changed with both increasing dietary methionine and arginine, dose-dependently.

Usefulness of Urinary Creatinine/Urea Nitrogen Ratio as Indicator of Body Protein Catabolism in Dogs Fed Low Protein Diets

“…Therefore, the most important dietary amino acids are the essential amino acids arginine and methionine. Hasegawa et al found that UCrn excretion increased in a dose-dependent manner based on dietary levels of arginine and methionine (43). Although we compared the composition of amino acids in the LP, in which UCrn was elevated, to the amino-acid compositions in the SP and HP, we did not find larger quantities of arginine or methionine.…”

Effect of replacement different methionine levels and sources with betaine on blood metabolites, breast muscle morphology and immune response in heat-stressed broiler chickens

“…In our study, plasma creatinine levels increased with increasing Met levels from deficient (Low-Met) to 30% higher (High-Met). Hasegawa et al (2017) declared that Met or arginine might become a limiting amino acid for creatine synthesis when Met or arginine was deficient in the diet. Other creatine precursors might become limiting amino acids for creatine biosynthesis when Met or arginine was excess in diet.…”

Measurement of urinary mesotocin in large-billed crows by enzyme-linked immunosorbent assay

“…For the analysis, MT concentration was represented as a ratio of MT to creatinine in order to control for the quantity of water in the urine samples, which enables the comparison of MT levels between individuals [ 4 , 18 , 24 , 29 ]. The creatinine concentration of each urine sample was measured using a commercially available kit, which was previously used for droppings of other avian species [ 5 , 8 ], based on the Jaffe reaction method (Item No. 500701, Cayman Chemicals).…”