Effects of Dietary Mannan-Oligosaccharide (Mos) and Multi-Species of Bacillus on Growth and Feed Utilization in Leopard Coral Grouper Plectropomus leopardus Juvenile

Abstract

This study assessed the growth and feed utilization in leopard coral grouper Plectropomus leopardus juvenile fed dietary prebiotic mannan-oligosaccharide (MOS) and multi-species of probiotic bacteria (Bacillus cereus BS6, B. subtilis BS3, and B. amiloliquefaciens BS4). The experiment was performed by feeding four replicates groups of juveniles weighing 3.61±0.60 g on four experimental diets, and each diet included: only MOS (PRE); the multi-species of Bacillus (PRO); a combination of MOS and the multi-species of Bacillus (SYN) and without supplementation (CON). When MOS (PRE) or the multi-species of Bacillus (PRO) was included in the diet solely, the growth of the fish was fairly good, although there was no significant difference with the control diet (p>0.05). Unexpectedly, the combined inclusion of MOS and the multi-species of Bacillus (SYN) did not increase the growth and feed utilization in Plectropomus leopardus juvenile. Presumably, MOS did not exert any favorable effects on the multi-species of Bacillus. This study suggested that no advantage was obtained when MOS and the multi-species of Bacillus were included in the diet simultaneously. In addition, the inclusion of MOS in higher levels may be necessary for better feed utilization and growth in leopard coral grouper Plectropomus leopardus.

Effects of dietary supplementation with mannan-rich oligosaccharides and solid-state fermented Aspergillus niger on the growth performance of juvenile Asian seabass, Lates calcarifer

“…These findings align with previous studies conducted on rainbow trout (Oncorhynchus mykiss (Walbaum)) fed with MOS 0.2% (Mínguez et al 2016), milkfish (Chanos chanos (Fabricius)) fed with MOS 0.3% (Harikrishnan et al 2023), and Atlantic salmon (Salmo salar L.) fed with MOS concentrations of 0.5% and 1.0% (Kazlauskaite et al 2022). MOS has been documented to improve functional gut health by increasing digestive enzyme activity to boost nutrient digestibility and the absorption of lipids, proteins, and phosphorus for better growth performance with a lower FCR (Flores-Kossack et al 2020, El-Saadony et al 2021, Xue et al 2022, Sudewi et al 2023. Electron microscopy and transmission electron microscopy showed that gilthead seabream (Sparus aurata L.) supplemented with MOS 0.2% had a greater capacity for nutrient capture Gary Petol Felix et al 39 thanks to an increase in microvilli density and length in both the anterior and posterior gut regions (Dimitroglou et al 2010).…”

“…Among all the treatments, significant growth improvement with the highest nutrient utilization was noted in the juvenile Asian seabass fed SSF 0.025% + MOS 0.05%, which can likely be attributed to multiple beneficial functions of each of the feed additives. The literature indicates that MOS acts as a prebiotic that stimulates the growth and colonization activity of beneficial bacteria in fish guts (El-Saadony et al 2021Flores-Kossack et al 2020, Sudewi et al 2023, Xue et al 2022, whereas Aspergillus niger, through solid-state fermentation (SSF), produces a range of enzymes that break down complex carbohydrates, proteins, and fibers of aquafeed into simpler, more digestible forms.…”

“…Therefore, the present study explored the potential combined effects of two feed additives, namely mannan oligosaccharide (MOS) and enzymes derived from the solid-state fermentation of Aspergillus niger (SSF), in the diet of juvenile Asian seabass. MOS, originating from the cell wall of Saccharomyces cerevisiae, is resistant to digestive enzyme degradation and can reach the distal intestine to promote the colonization of beneficial gut bacteria (Sudewi et al 2023). On the other hand, SSF produces various hydrolytic enzymes that break down complex substrates to promote nutrient release (Jannathulla and Dayal 2022).…”