Effects of dietary lipid levels on growth performance, hepatic health, lipid metabolism and intestinal microbiota on
            <i>Trachinotus ovatus</i>

Fang, Hao‐Hang; Zhao, Wei; Xie, Jiajun; Yin, Peng; Zhuang, Zhenxiao; Liu, Yongjian; Liu, Tian; Niu, Jin

doi:10.1111/anu.13296

Cited by 16 publications

(11 citation statements)

References 68 publications

(77 reference statements)

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“…Studies have shown that activation of pparα could promote fatty acid β-oxidation by promoting the expression of cpt1, which is the rate-limiting enzyme for fatty acid oxidation [57,58]. For small and medium turbot, expressions of pparα and cpt1 in the L171.2 group were significantly lower than that in other groups, similar to a previous study in Trachinotus ovatus, demonstrating that excessive lipid intake might reduce hepatic lipolysis by the pparα-cpt1 signal pathway [59]. Nevertheless, experimental diets had no significant effect on expressions of pparα and cpt1 in large turbot, which might be due to the greater tolerance to high lipids of the large turbot.…”

Section: Discussionsupporting

confidence: 86%

Effects of Dietary Lipid Levels on Growth, Digestive Enzyme Activities, Antioxidant Capacity, and Lipid Metabolism in Turbot (Scophthalmus maximus L.) at Three Different Stages

Zhang

Dan

Zhuang

et al. 2022

Aquaculture Nutrition

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Three 56-day feeding trials were conducted to evaluate dietary lipid requirements and effects of dietary lipid levels on growth, digestive enzyme activities, antioxidant capacity, and lipid metabolism in turbot (Scophthalmus maximus L.) at three growth stages. The initial mean body weight of turbot was 9.13 ± 0.17 g, 50.10 ± 0.38 g, and 80.05 ± 0.76 g (small, medium, and large turbot), respectively. Five practical diets were formulated to contain 68.4, 93.9, 120.7, 147.8, and 171.2 g/kg lipid (L68.4, L93.9, L120.7, L147.8, and L171.2), respectively. Results of three trials showed that the weight gain rate and specific growth rate of turbot fed with L120.7, L147.8, and L171.2 diets were all significantly higher compared with turbot fed with L68.4 and L93.9 diets except for large turbot fed with the L147.8 diet. Activities of intestinal trypsin, lipase and hepatic catalase, superoxide dismutase, and total antioxidant capacity firstly increased and then decreased as dietary lipids increased. Meanwhile, malondialdehyde content decreased firstly but then increased with the elevation of dietary lipids in small and medium turbot, and it was significantly higher in the L171.2 group than the rest in large turbot. With increasing levels of dietary lipid, contents of whole-body lipid, liver lipid, serum total cholesterol, triglyceride, and low-density lipoprotein cholesterol were markedly increased at three stages. In addition, serum high-density lipoprotein cholesterol contents increased firstly and then decreased over the L120.7 group. Transcriptional levels of lipolysis-related genes lipin1 and lpl were significantly upregulated firstly and subsequently downregulated with increasing dietary lipid levels except lipin1 in medium fish. Meanwhile, a significant increase in lipogenesis-related genes lxr and pparγ expressions was detected in all fish. Based on the specific growth rate, the dietary lipid level of 130.1, 120.1, and 107.7 g/kg was optimal for the growth performance of turbot cultured at three phases, respectively. Additionally, the results indicated that high lipid diets may lead to abnormal lipid deposition and affect the physiological health of turbot.

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Section: Discussionsupporting

confidence: 86%

Effects of Dietary Lipid Levels on Growth, Digestive Enzyme Activities, Antioxidant Capacity, and Lipid Metabolism in Turbot (Scophthalmus maximus L.) at Three Different Stages

Zhang

Dan

Zhuang

et al. 2022

Aquaculture Nutrition

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“…In consideration of these results, the TLR signaling pathway may play a role in the potential effects of LPC supplementation on intestinal physiology. Meanwhile, as known, high-lipid diet-induced intestinal damage was associated with the disruption of the intestinal mucosal barrier in both humans and fish [ 15 , 45 ]. Previous studies in Nile tilapia ( Oreochromis niloticus ) and rice field eel ( Monopterus albus ) have demonstrated that high-lipid diets can stimulate the TLR/NF-κB signaling pathway to regulate the expression of inflammatory cytokines [ 11 , 17 ].…”

Section: Discussionmentioning

confidence: 99%

“…In modern aquaculture, in order to spare dietary protein, the use of high-lipid diets is becoming more and more popular. However, a variety of problems resulting from high-lipid diets have occurred, including damage of fish intestines [ 13 , 14 , 15 ]. Accumulating evidence indicates that intestinal inflammation induced by high levels of dietary lipid is associated with TLR-signal transduction in both mammals and fish [ 16 , 17 , 18 ].…”

Section: Introductionmentioning

confidence: 99%

Effects of Lysophosphatidylcholine on Intestinal Health of Turbot Fed High-Lipid Diets

Luo

Liao

et al. 2022

Nutrients

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An 8-week feeding trial was conducted, where turbot were fed four experimental diets, containing different LPC levels (0%, 0.1%, 0.25%, and 0.5%, named LPC0, LPC0.1, LPC0.25, and LPC0.5, respectively). The intestinal morphology results showed that there were no widened lamina propria and mixed inflammatory cells in the LPC-supplemented groups. Dietary LPC remarkably decreased the expression of TLRs (TLR3, TLR8, TLR9, and TLR22), MyD88, and signaling molecules (NF-κB, JNK, and AP-1). Similarly, diets with LPC supplementation markedly depressed the gene expression of NF-κB and JNK signaling pathway downstream genes (TNF-α, IL-1β, Bax, Caspase9, and Caspase-3). Furthermore, dietary LPC modified the intestinal microbial profiles, increasing the relative abundance of short-chain fatty acids-producers, lactic acid bacteria, and digestive enzyme-producing bacteria. Predictive functions of intestinal microbiota showed that turbot fed LPC diets had a relatively higher abundance of functions, such as lipid metabolism and immune system, but a lower abundance of functions, such as metabolic diseases and immune system diseases. The activities of intestinal acid phosphatase and alkaline phosphatase were also increased by dietary LPC. In conclusion, LPC supplementation could regulate the intestinal mucosal barrier via the TLR signaling pathway and alter the intestinal microbiota profile of turbot fed high-lipid diets.

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“…Hepatopancreas samples were homogenized and centrifuged according to the methods of Fang et al, 2021b). Briefly, hepatopancreas samples and phosphate buffer (1:10) were mixed and homogenized, and then centrifuged for 10 min (4 • C, 1,200 g), and the supernatants were collected.…”

Section: Detection Of Hepatopancreas and Hemolymph Antioxidant Parametersmentioning

confidence: 99%

Replacement of Astaxanthin With Lutein in Diets of Juvenile Litopenaeus vannamei: Effects on Growth Performance, Antioxidant Capacity, and Immune Response

Fang

Zeng²,

Liu

et al. 2021

Front. Mar. Sci.

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An 8-week feeding experiment was conducted to investigate whether diet supplementation of lutein could result in similar growth performance, antioxidant capacity, and immunity of Litopenaeus vannamei when compared to dietary astaxanthin. Juvenile L. vannamei (initial body weight: 0.64 ± 0.04 g) were fed with one of five isonitrogen and isolipids diets with/without lutein or astaxanthin [control group (C); the lutein (L) groups contained 0, 62.5, 75, 87.5 ppm lutein, respectively, the astaxanthin (A) group contained 50 ppm astaxanthin]. Results showed that dietary supplementation of lutein ranging from 62.5 to 75 ppm resulted in similar growth performance (WGR, SGR, FCR, and SR) of L. vannamei compared with the A group (P > 0.05). Apart from that, no statistical difference was observed in antioxidant parameters (hemolymph T-AOC, hemolymph MDA, and RNA expression level of GSH-PX, CAT), anti-inflammatory ability (Relish, Rho, and HSP70) and apoptosis-related gene expression (Caspase3) among lutein treatments ranging from 62.5 to 87.5 ppm and the A group (P > 0.05). These results indicate that a dose of 62.5–75 ppm of lutein was suitable in the diet of L. vannamei for substituting dietary astaxanthin.

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Effects of dietary lipid levels on growth performance, hepatic health, lipid metabolism and intestinal microbiota on Trachinotus ovatus

Cited by 16 publications

References 68 publications

Effects of Dietary Lipid Levels on Growth, Digestive Enzyme Activities, Antioxidant Capacity, and Lipid Metabolism in Turbot (Scophthalmus maximus L.) at Three Different Stages

Effects of Dietary Lipid Levels on Growth, Digestive Enzyme Activities, Antioxidant Capacity, and Lipid Metabolism in Turbot (Scophthalmus maximus L.) at Three Different Stages

Effects of Lysophosphatidylcholine on Intestinal Health of Turbot Fed High-Lipid Diets

Replacement of Astaxanthin With Lutein in Diets of Juvenile Litopenaeus vannamei: Effects on Growth Performance, Antioxidant Capacity, and Immune Response

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