Effects of dietary l-methionine supplementation on intestinal integrity and oxidative status in intrauterine growth-retarded weanling piglets

Su, W.P. Daniel; Zhang, Hao; Ying, Zhixiong; Li, Yue; Zhou, Le; Wang, Fei; Zhang, Lili

doi:10.1007/s00394-017-1539-3

Cited by 58 publications

(54 citation statements)

References 51 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The balance of cell apoptosis and proliferation are important for maintaining the turnover of the intestinal mucosal epithelium [55]. Inflammation and oxidative stress can induce excessive apoptosis and declined proliferation, which could lead to intestinal barrier dysfunction [56,57]. In the present study, we observed apoptosis was promoted in the intestinal mucosae of LPS-challenged broilers using a TUNEL assay.…”

Section: Discussionsupporting

confidence: 48%

Dietary Stevioside Supplementation Alleviates Lipopolysaccharide-Induced Intestinal Mucosal Damage through Anti-Inflammatory and Antioxidant Effects in Broiler Chickens

Jiang

et al. 2019

Antioxidants

View full text Add to dashboard Cite

The study was conducted to investigate the effects of dietary stevioside (STE) supplementation on the lipopolysaccharide (LPS)-induced intestinal mucosal damage of broiler chickens. A total of 192 one-day-old male Ross 308 broiler chicks were randomly divided into four treatments: (1) basal diet (CON); (2) basal diet supplemented with 250 mg/kg stevioside (STE);(3) basal diet + LPS-challenge (LPS); (4) basal diet supplemented with 250 mg/kg stevioside + LPS-challenge (LPS + STE). LPS-challenged groups received an intraperitoneal injection of LPS at 17, 19 and 21 d, whereas the CON and STE groups received a saline injection. The results showed that dietary STE supplementation normalized LPS-induced changes in protein expression of p-NF-κB and p-IκBα, mRNA expression of inflammatory genes (TLR4, NF-κB, and IFN-γ), tight junction-related genes (CLDN2, OCLN, and ZO-1), and antioxidant genes (Nrf2 and HO-1). LPS-induced decreases in serum diamine oxidase (DAO) level, villus height-to-crypt depth ratio, apoptotic index, and protein expression of proliferating cell nuclear antigen (PCNA) were reversed with dietary STE supplementation. Additionally, STE supplementation ameliorated the redox damage by reducing malondialdehyde (MDA) content and increasing total antioxidant capacity (T-AOC) and antioxidant enzyme activity. In conclusion, dietary stevioside supplementation could alleviate LPS-induced intestinal mucosal damage through anti-inflammatory and antioxidant effects in broiler chickens.Antioxidants 2019, 8, 575 2 of 20 and oxidative status of the intestine in broiler chickens [7][8][9]. Thus, LPS has been widely applied to establish a model of intestinal mucosal damage in broiler chickens [2,[7][8][9][10]. It is necessary to explore feed additives with therapeutic potential for disrupted intestinal homeostasis of LPS-challenged in broilers.Stevioside (STE) is a natural diterpenoid glycoside extracted from the Stevia rebaudiana (Bertoni), which has been proven to be safe in the food industry [11]. A previous study has suggested that STE exerts no harmful effects in the chicken diet with a dose of 667 mg/kg [12]. Despite STE being 250−300 times sweeter than sucrose, it has several nutritional and medical activities such as anti-hyperglycaemic [13], anti-hypertensive [14], and anti-tumor activities [15]. Furthermore, several studies have shown that STE exerts anti-inflammatory and antioxidant effects both in vivo and in vitro [11,14,16]. In rats, STE could prevent liver inflammation through antioxidant activity by activating Nrf2 and anti-inflammatory activity by suppressing NF-κB signaling [11]. In a human colonic cell line, steviol (a derivative of STE) suppressed the IL-8 release induced by TNF-α, and reduced the protein expression of NF-κB [17]. STE could also attenuate the LPS-induced synthesis of pro-inflammatory cytokines by regulating IκBα/NF-κB signaling pathway [16]. In mice, STE promoted macrophage function and increased humoral immune response [18]. STE treatment enhanced antioxidant defense in both the adi...

show abstract

Section: Discussionsupporting

confidence: 48%

Dietary Stevioside Supplementation Alleviates Lipopolysaccharide-Induced Intestinal Mucosal Damage through Anti-Inflammatory and Antioxidant Effects in Broiler Chickens

Jiang

et al. 2019

Antioxidants

View full text Add to dashboard Cite

show abstract

“…The jejunum is not only the main place for the digestion and absorption of nutrients, but also the immune organ in the body [5]. Recent results have demonstrated that IUGR can cause jejunum damage in piglets at 21 and 50 days [6][7][8]. As for the IUGR programming consequences, IUGR might affect the jejunum function of growing pigs, such as immunization, intestinal barrier, proliferation, and apoptosis, but few relevant reports have been reported so far.…”

Section: Introductionmentioning

confidence: 99%

“…The effect of IUGR on jejunum function is closely related to oxidative function. For example, previous research proved that imbalance of antioxidant homeostasis is an important factor contributing to intestinal mucosal injury induced by IUGR [8]. However, few effective methods to alleviate jejunum damage and oxidative stress in IUGR pigs have been found.…”

Section: Introductionmentioning

confidence: 99%

Curcumin Alleviates IUGR Jejunum Damage by Increasing Antioxidant Capacity through Nrf2/Keap1 Pathway in Growing Pigs

Yan

Zhang

Han

et al. 2019

Animals

Self Cite

View full text Add to dashboard Cite

The purpose of this study was to explore the effects of curcumin on IUGR jejunum damage. A total of 24 IUGR and 12 normal-birth weight (NBW) female crossbred (Duroc × Landrace × Large White) piglets were randomly assigned into three groups at weaning (26 days): IUGR group, NBW group, and IUGR + CUR group, which were fed diets containing 0 mg/kg (NBW), 0 mg/kg (IUGR) and 200 mg/kg (IUGR + CUR) curcumin from 26 to 115 days of age. Results showed that dietary supplementation with 200 mg/kg curcumin significantly increased the total superoxide dismutase (T-SOD) activity and decreased the malondialdehyde (MDA) content in the jejunum of IUGR pigs (p < 0.05). Results of real-time PCR showed that the IUGR + CUR group significantly increased the gene expression of NF-E2-related factor 2 (Nrf2) (p < 0.05), and increased the glutamate-cysteine ligase catalytic subunit (GCLC), superoxide dismutase 1 (SOD1), glutamate-cysteine ligase modifier subunit (GCLM), and NAD(P)H quinone dehydrogenase 1 (NQO1) mRNA expression compared with the IUGR group (p < 0.05). Western blot results showed that dietary supplementation with 200 mg/kg curcumin significantly increased the protein levels of Nrf2 and NQO1. Compared with the IUGR group, pigs in IUGR + CUR group showed significantly decreased the levels of tumor necrosis factor-α (TNFα), interleukin-6 (IL-6), and interferon gamma (IFNγ) (p < 0.05), and increased the interleukin-2 (IL-2) level (p < 0.05). Dietary supplementation with 200 mg/kg curcumin significantly reduced cysteinyl aspartate specific proteinase 3 (caspase3), BCL2-associated X protein (bax), B-cellCLL/lymphoma 2 (bcl2), and heat-shock protein 70 (hsp70) mRNA expression, and increased occludin (ocln) mRNA expression (p < 0.05). In conclusion, dietary supplementation with 200 mg/kg curcumin can alleviate jejunum damage in IUGR growing pigs, through Nrf2/Keap1 pathway.

show abstract

“…The VH (villus height), VW (villus weight) and CD (crypt depth) were measured by image‐pro plus software. The VSA (villus surface area) was calculated as follows (Su et al, ):

VSA = π \times \frac{VW}{2} \sqrt{{()(, \frac{VM}{2})}^{2} + {VH}^{2}}

…”

Section: Methodsmentioning

confidence: 99%

“…The intestine is a crucial organ essential for nutrient absorption and bile and waste excretion and an important site of host immunity (Chin, Hill, Aurora, & Spence, ). Adequate dietary Met is necessary for optimal protein synthesis and mucosal integrity, as well as the redox status in the small intestine in humans and animals (Chen et al, ; Su et al, ). A number of studies have demonstrated that Met deficiency suppresses protein synthesis and epithelial cell growth (Bauchart‐Thevret, Stoll, Chacko, & Burrin, ; Tang et al, ), and is relevant to overall health and wellbeing.…”

Section: Introductionmentioning

confidence: 99%

Effects of methionine partially replaced by methionyl‐methionine dipeptide on intestinal function in methionine‐deficient pregnant mice

Chen

Wang

Zhao

et al. 2019

Animal Physiology Nutrition

View full text Add to dashboard Cite

This study was to compare the effects of parenteral supplementation of methionyl‐methionine (Met‐Met) or Met on intestinal barrier function in Met‐deficient pregnant mice. Pregnant mice were randomly divided into three groups. The Control group was provided a diet containing Met and received i.p. injection of saline. The Met group was fed the same diet but without Met and received daily i.p. injection of 35% of the Met contained in the control diet. The Met‐Met group was treated the same as the Met group, except that 25% of the Met injected was replaced with Met‐Met. Met‐Met promoted villus surface area in ileum compared with Met alone. In addition, the mRNA abundance of amino acid and glucose transporters in the small intestine was altered with Met‐Met. Moreover, Met‐Met increased tight junction protein and decreased apoptosis‐related proteins expression in the jejunum and ileum. These results suggest that Met‐Met can promote intestinal function over Met alone in Met‐deficient mice.

show abstract

Effects of dietary l-methionine supplementation on intestinal integrity and oxidative status in intrauterine growth-retarded weanling piglets

Abstract: Methionine may have beneficial effects in improving intestinal integrity and oxidative status in IUGR weanling piglets.

Cited by 58 publications

References 51 publications

Dietary Stevioside Supplementation Alleviates Lipopolysaccharide-Induced Intestinal Mucosal Damage through Anti-Inflammatory and Antioxidant Effects in Broiler Chickens

Dietary Stevioside Supplementation Alleviates Lipopolysaccharide-Induced Intestinal Mucosal Damage through Anti-Inflammatory and Antioxidant Effects in Broiler Chickens

Curcumin Alleviates IUGR Jejunum Damage by Increasing Antioxidant Capacity through Nrf2/Keap1 Pathway in Growing Pigs

Effects of methionine partially replaced by methionyl‐methionine dipeptide on intestinal function in methionine‐deficient pregnant mice

Contact Info

Product

Resources

About