Effects of dietary L-lysine supplementation on lysine transport by the piglet small intestine in vitro1

He, Liuqin; Niu, Huanqing; Li, H.; Xu, Zhiqi; Yao, Kang; Li, T. J.; Yin, Yulong

doi:10.2527/jas.2015-0207

Cited by 5 publications

(2 citation statements)

References 16 publications

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“…β-Actin and GAPDH were used as housekeeping genes to normalize target gene transcript levels. Real-time PCR was performed as described previously . Relative gene expression was normalized and expressed as a ratio of the target gene to the control gene using the formula 2 –(ΔΔCt) , where ΔΔCt = (Ct Target – Ct β‑actin/GADPH ) treatment – (Ct Target – Ct β‑actin/GADPH ) control .…”

Section: Methodsmentioning

confidence: 99%

AMPK/α-Ketoglutarate Axis Regulates Intestinal Water and Ion Homeostasis in Young Pigs

Huang

et al. 2017

J. Agric. Food Chem.

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Water and ion absorption via sensitive aquaporins (AQPs) and ion channels is of critical importance in intestinal health. However, whether α-ketoglutarate (AKG) could improve intestinal water and ion homeostasis in lipopolysaccharide (LPS)-challenged piglets and whether the AMP-activated protein kinase (AMPK) pathway is involved remains largely unknown. This study was conducted to investigate the effect of dietary AKG supplementation on the small intestinal water and ion homeostasis through modulating the AMPK pathway in a piglet diarrhea model. A total of 32 weaned piglets were used in a 2 × 2 factorial design; the major factors were diet (basal diet or 1% AKG diet) and challenge (Escherichia coli LPS or saline). The results showed that LPS challenge increased the diarrhea index and affected the concentrations of serum Na, K, Cl, glucose, and AKG and its metabolites in piglets fed the basal or AKG diet. However, the addition of AKG attenuated diarrhea incidence and reversed these serum parameter concentrations. Most AQPs (e.g., AQP1, AQP3, AQP4, AQP5, AQP8, AQP10, and AQP11) and ion transporters (NHE3, ENaC, and DRA/PAT1) were widely distributed in the duodenum and jejunum of piglets. We also found that AKG up-regulated the expression of intestinal epithelial AQPs while inhibiting the expression of ion transporters. LPS challenge decreased (P < 0.05) the gene and protein expression of the AMPK pathway (AMPKα1, AMPKα2, SIRT1, PGC-1α, ACC, and TORC2) in the jejunum and ileum. Notably, AKG supplementation enhanced the abundance of these proteins in the LPS-challenged piglets. Collectively, AKG plays an important role in increasing water and ion homeostasis through modulating the AMPK pathway. Our novel finding has important implications for the prevention and treatment of gut dysfunction in neonates.

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Section: Methodsmentioning

confidence: 99%

AMPK/α-Ketoglutarate Axis Regulates Intestinal Water and Ion Homeostasis in Young Pigs

Huang

et al. 2017

J. Agric. Food Chem.

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“…Villous surface implies the intestinal absorptive capacity of nutrients ( 26 ). Our previous study has shown that Lys was involved in enhancing intestinal crypt height and villus depth and differentially affected intestinal cationic amino acid transporter expression ( 11 , 27 ). However, our present results showed that Lys-Lys had no effect on villous height, indicating that Lys-Lys does not affect the intestinal absorptive capacity of suckling piglets.…”

Section: Discussionmentioning

confidence: 99%

Effects of Lysine–Lysine Dipeptide on Serum Amino Acid Profiles, Intestinal Morphology, and Microbiome in Suckling Piglets

Deng

Han

et al. 2022

Front. Nutr.

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AimsSmall peptides are more energy-saving and efficiently absorbed compared to amino acids. Our study aimed to evaluate the effect of the Lys-Lys dipeptide on the improvement of growth performance, amino acid metabolism, and gut development in suckling piglets.Methods and ResultsTwenty-eight newborn suckling piglets were orally administrated with 0.1%, 1%, and 5% Lys-Lys dipeptide for 21 days. Our results showed that the Lys-Lys dipeptide has no significant effect on growth performance and intestinal morphology compared with the control group. We also found that the 1% Lys-Lys dipeptide significantly increased the concentrations of serum Lys, Thr, Phe, and Pro while decreasing Cys compared to the control group. Similarly, the 5% Lys-Lys dipeptide markedly increased the concentrations of serum Lys, Iso, Thr, Asp, Glu, and Pro compared to the control group. Moreover, the Lys-Lys dipeptide downregulated the expression of jejunal Slc7a1, Slc7a2, and Slc15a1 and ileal Slc7a2. Additionally, the Lys-Lys dipeptide decreased the microbiota richness indices and relative abundance of Bacteroidales.ConclusionIn this study, we found that the Lys-Lys dipeptide contributes to the metabolism of amino acids but failed to affect the growth performance of piglets. Additionally, the Lys-Lys dipeptide decreased the relative abundance of Bacteroidales. These results provide a theoretical for the future application and research of Lys-Lys dipeptide in intestinal development of suckling piglets.

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Metabolic growth, plankton selectivity, haemato-biochemical and intestinal morphometry of Nile tilapia (Oreochromis niloticus) fed a lysine-deficient diet in earthen ponds

Khalil

Momoh

Al-Kenawy

et al. 2022

Aquaculture Reports

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Effects of dietary L-lysine supplementation on lysine transport by the piglet small intestine in vitro1

Cited by 5 publications

References 16 publications

AMPK/α-Ketoglutarate Axis Regulates Intestinal Water and Ion Homeostasis in Young Pigs

AMPK/α-Ketoglutarate Axis Regulates Intestinal Water and Ion Homeostasis in Young Pigs

Effects of Lysine–Lysine Dipeptide on Serum Amino Acid Profiles, Intestinal Morphology, and Microbiome in Suckling Piglets

Metabolic growth, plankton selectivity, haemato-biochemical and intestinal morphometry of Nile tilapia (Oreochromis niloticus) fed a lysine-deficient diet in earthen ponds

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