Effects of dietary L-citrulline supplementation on nitric oxide synthesis, immune responses and mitochondrial energetics of broilers during heat stress

Uyanga, Victoria Anthony; Zhao, Jingpeng; Wang, Xiaojuan; Jiao, Hongchao; Onagbesan, Okanlawon

doi:10.1016/j.jtherbio.2022.103227

Cited by 7 publications

(17 citation statements)

References 58 publications

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“…Another study (Uyanga, Zhao, et al, 2022) investigated whether dietary supplementation with L‐Cit (so that it provides 1% of the basal diet) could ameliorate the effects of acute heat stress on broilers, as well as help with redox balance and inflammatory responses. Ross 308 broilers (288 chicks) were subjected to one of two environments: CT (24°C) or HT (35°C) for 5 h. They were fed one of two diets: control or L‐Cit.…”

Section: L‐cit and Thermotolerance In Layer Chicks And Broiler Chickensmentioning

confidence: 99%

“…Therefore, Cit has not been considered to add in the poultry diet. However, recent studies showed that Cit administration can minimize heat stress in chickens (Chowdhury et al, 2017; Chowdhury, Ouchi, Han, et al, 2021; Uyanga, Liu, et al, 2022; Uyanga, Zhao, et al, 2022). Moreover, a concern with dietary Arg supplementation is its potential antagonism with basic amino acids, including Arg, lysine, and histidine, whereas Cit is a neutral amino acid and does not share the same transporters with basic amino acids (Wu & Morris, 1998).…”

Section: Future Perspectivesmentioning

confidence: 99%

“…Nevertheless, our findings suggest that NO may not be the main factor in the reduction in body temperature and thermotolerance that results from the oral administration of L‐Cit, which was confirmed by NOS inhibitor, N G ‐nitro‐L‐arginine methyl ester hydrochloride (Figure 1; Chowdhury et al, 2017). However, Uyanga, Zhao, et al (2022) showed that when L‐Cit is injected centrally, but not intraperitoneally, there is a reduction in body temperature and an increase in hypothalamic NO concentration and NOS synthesis. Additionally, dietary administration of 1% L‐Cit in broilers from 1 to 42 days old caused a lowering of rectal temperature under CT and an increase in NOS isoforms (Uyanga, Zhao, et al, 2022).…”

Section: L‐cit In Thermoregulationmentioning

confidence: 99%

“…However, Uyanga, Zhao, et al (2022) showed that when L‐Cit is injected centrally, but not intraperitoneally, there is a reduction in body temperature and an increase in hypothalamic NO concentration and NOS synthesis. Additionally, dietary administration of 1% L‐Cit in broilers from 1 to 42 days old caused a lowering of rectal temperature under CT and an increase in NOS isoforms (Uyanga, Zhao, et al, 2022). Thus, L‐Cit may or may not change the NO concentration, depending on which subjects are being administered, as well as the means of administration (Figure 2).…”

Section: L‐cit In Thermoregulationmentioning

confidence: 99%

“…However, supplementation with L‐Cit resulted in the upregulation of the mitochondrial transcription factor A, and during HT, L‐Cit increased ATP5β expression to a similar level to that of CT‐housed broilers. It has thus been demonstrated that dietary L‐Cit can promote muscle ATP generation during heat stress in broilers (Uyanga, Zhao, et al, 2022). It has also been reported that oral administration of L‐Cit caused an increase in the liver of both NADH and NAD + , which are electron carriers and may increase oxidative phosphorylation activity in the mitochondria (Chowdhury, Ouchi, Haraguchi, & Bungo, 2021).…”

Section: L‐cit and Thermotolerance In Layer Chicks And Broiler Chickensmentioning

confidence: 99%

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L‐Citrulline: A novel hypothermic amino acid promoting thermotolerance in heat‐exposed chickens

Chowdhury

2023

Animal Science Journal

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With global warming becoming of increasing concern, poultry farms are experiencing a concomitant increase in heat stress. Chickens are very sensitive to high ambient temperature (HT), so the development of novel nutrients that will help deal with the challenge posed by heat stress is vital. We revealed that L‐citrulline (L‐Cit) can reduce body temperature in chickens. Orally administered L‐Cit solution has been found to provide heat tolerance in chickens and to result in reduced food intake. Heat exposure and oral administration of L‐Cit led to increased levels of plasma insulin, whereas heat stress led to a decline in plasma thyroxine. Dietary administration of L‐Cit was also shown to be effective to reduce heat stress in broiler chickens. Moreover, L‐Cit was found to be metabolized in the liver within 1 h of its administration, and in L‐Cit‐treated broiler chicks, the Cit‐Arginine cycle and the Krebs cycle were found to be active. L‐Cit has not yet been approved for inclusion in the poultry diet, so it is important to find alternative sources of L‐Cit. Taken together, these findings suggest that L‐Cit may serve as an important novel nutrient with the ability to produce heat tolerance in chickens under HT.

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Section: L‐cit and Thermotolerance In Layer Chicks And Broiler Chickensmentioning

confidence: 99%

Section: Future Perspectivesmentioning

confidence: 99%

Section: L‐cit In Thermoregulationmentioning

confidence: 99%

Section: L‐cit In Thermoregulationmentioning

confidence: 99%

Section: L‐cit and Thermotolerance In Layer Chicks And Broiler Chickensmentioning

confidence: 99%

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L‐Citrulline: A novel hypothermic amino acid promoting thermotolerance in heat‐exposed chickens

Chowdhury

2023

Animal Science Journal

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L‐Citrulline Ameliorates Iron Metabolism and Mitochondrial Quality Control via Activating AMPK Pathway in Intestine and Improves Microbiota in Mice with Iron Overload

Zhao,

Gao,

Chen

et al. 2024

Molecular Nutrition Food Res

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ScopeOxidative stress caused by iron overload tends to result in intestinal mucosal barrier dysfunction and intestinal microbiota imbalance. As a neutral and nonprotein amino acid, L‐Citrulline (L‐cit) has been implicated in antioxidant and mitochondrial amelioration properties. This study investigates whether L‐cit can alleviate iron overload‐induced intestinal injury and explores the underlying mechanisms.Methods and resultsC57BL/6J mice are intraperitoneally injected with iron dextran, then gavaged with different dose of L‐cit for 2 weeks. L‐cit treatment significantly alleviates intestine pathological injury, oxidative stress, ATP level, and mitochondrial respiratory chain complex activities, accompanied by ameliorating mitochondrial quality control. L‐cit‐mediated protection is associated with the upregulation of Glutathione Peroxidase 4 (GPX4) expression, inhibition Nuclear Receptor Coactivator 4 (NCOA4)‐mediated ferritinophagy and ferroptosis, and improvement of gut microbiota. To investigate the underlying molecular mechanisms, Intestinal Porcine Epithelial Cell line‐J2 (IPEC‐J2) cells are treated with L‐cit or AMP‐activated Protein Kinase (AMPK) inhibitor. AMPK signaling has been activated by L‐cit. Notably, Compound C abolishes L‐cit's protection on intestinal barrier, mitochondrial function, and antioxidative capacity in IPEC‐J2 cells.ConclusionL‐cit may restrain ferritinophagy and ferroptosis to regulate iron metabolism, and induce AMPK pathway activation, which contributes to exert antioxidation, ameliorate iron metabolism and mitochondrial quality control, and improve intestinal microbiota. L‐cit is a promising therapeutic strategy for iron overload‐induced intestinal injury.

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Dietary Supplementation of Brevibacillus laterosporus S62-9 Improves Broiler Growth and Immunity by Regulating Cecal Microbiota and Metabolites

Zhi

Liu

et al. 2023

Probiotics & Antimicro. Prot.

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Effects of dietary L-citrulline supplementation on nitric oxide synthesis, immune responses and mitochondrial energetics of broilers during heat stress

Cited by 7 publications

References 58 publications

L‐Citrulline: A novel hypothermic amino acid promoting thermotolerance in heat‐exposed chickens

L‐Citrulline: A novel hypothermic amino acid promoting thermotolerance in heat‐exposed chickens

L‐Citrulline Ameliorates Iron Metabolism and Mitochondrial Quality Control via Activating AMPK Pathway in Intestine and Improves Microbiota in Mice with Iron Overload

Dietary Supplementation of Brevibacillus laterosporus S62-9 Improves Broiler Growth and Immunity by Regulating Cecal Microbiota and Metabolites

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